Expression and characterization of a cold-adapted lipase from an antarctic Pseudomonas sp.

• Main Author: Ganasen, Menega
• Format: Thesis
• Language: English
• Published: 2014
• Subjects: Lipase; Pseudomonas; Enzymes - Purification; Antarctic; Tac; Antarc*; Antarctica
• Online Access: http://psasir.upm.edu.my/id/eprint/90649/; http://psasir.upm.edu.my/id/eprint/90649/1/FBSB%202014%2044%20-%20IR.pdf

Eleven strains of unknown microbial isolates obtained from Antarctica were screened for extracellular lipolytic activity. Eight isolates showed positive results on Tributyrin, Rhodamine B and Triolein agar plates. A single isolate (designated as strain AMS8) that demonstrated the highest lipase acti...