Production of lipopeptide biosurfactant by a hydrocarbon-degrading antarctic Rhodococcus
Rhodococci are renowned for their great metabolic repertoire partly because of their numerous putative pathways for large number of specialized metabolites such as biosurfactant. Screening and genome-based assessment for the capacity to produce surface-active molecules was conducted on Rhodococcus s...
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Multidisciplinary Digital Publishing Institute
2020
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ftunivpmalaysia:oai:psasir.upm.edu.my:87319 2023-05-15T14:01:42+02:00 Production of lipopeptide biosurfactant by a hydrocarbon-degrading antarctic Rhodococcus Abdul Habib, Nur Muhamad Syahir Ahmad, Siti Aqlima Wan Johari, Wan Lutfi Abd. Shukor, Mohd Yunus Yasid, Nur Adeela Alias, Siti Aisyah Smykla, Jerzy Saruni, Nurul Hani Abdul Razak, Nur Syafiqah 2020-08-26 text http://psasir.upm.edu.my/id/eprint/87319/ http://psasir.upm.edu.my/id/eprint/87319/1/Production%20of%20lipopeptide%20biosurfactant.pdf https://onlinelibrary.wiley.com/toc/15480569/2018/39/1 en eng Multidisciplinary Digital Publishing Institute http://psasir.upm.edu.my/id/eprint/87319/1/Production%20of%20lipopeptide%20biosurfactant.pdf Abdul Habib, Nur Muhamad Syahir and Ahmad, Siti Aqlima and Wan Johari, Wan Lutfi and Abd. Shukor, Mohd Yunus and Yasid, Nur Adeela and Alias, Siti Aisyah and Smykla, Jerzy and Saruni, Nurul Hani and Abdul Razak, Nur Syafiqah (2020) Production of lipopeptide biosurfactant by a hydrocarbon-degrading antarctic Rhodococcus. International Journal of Molecular Sciences, 21 (17). pp. 1-21. ISSN 1422-0067 Article PeerReviewed 2020 ftunivpmalaysia 2022-09-06T17:23:57Z Rhodococci are renowned for their great metabolic repertoire partly because of their numerous putative pathways for large number of specialized metabolites such as biosurfactant. Screening and genome-based assessment for the capacity to produce surface-active molecules was conducted on Rhodococcus sp. ADL36, a diesel-degrading Antarctic bacterium. The strain showed a positive bacterial adhesion to hydrocarbon (BATH) assay, drop collapse test, oil displacement activity, microplate assay, maximal emulsification index at 45% and ability to reduce water surface tension to < 30 mN/m. The evaluation of the cell-free supernatant demonstrated its high stability across the temperature, pH and salinity gradient although no correlation was found between the surface and emulsification activity. Based on the positive relationship between the assessment of macromolecules content and infrared analysis, the extracted biosurfactant synthesized was classified as a lipopeptide. Prediction of the secondary metabolites in the non-ribosomal peptide synthetase (NRPS) clusters suggested the likelihood of the surface-active lipopeptide production in the strain’s genomic data. This is the third report of surface-active lipopeptide producers from this phylotype and the first from the polar region. The lipopeptide synthesized by ADL36 has the prospect to be an Antarctic remediation tool while furnishing a distinctive natural product for biotechnological application and research. Article in Journal/Newspaper Antarc* Antarctic Universiti Putra Malaysia: PSAS (Perpuskataan Sultan Abuld Samad) Institutional Repository Antarctic |
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Open Polar |
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Universiti Putra Malaysia: PSAS (Perpuskataan Sultan Abuld Samad) Institutional Repository |
op_collection_id |
ftunivpmalaysia |
language |
English |
description |
Rhodococci are renowned for their great metabolic repertoire partly because of their numerous putative pathways for large number of specialized metabolites such as biosurfactant. Screening and genome-based assessment for the capacity to produce surface-active molecules was conducted on Rhodococcus sp. ADL36, a diesel-degrading Antarctic bacterium. The strain showed a positive bacterial adhesion to hydrocarbon (BATH) assay, drop collapse test, oil displacement activity, microplate assay, maximal emulsification index at 45% and ability to reduce water surface tension to < 30 mN/m. The evaluation of the cell-free supernatant demonstrated its high stability across the temperature, pH and salinity gradient although no correlation was found between the surface and emulsification activity. Based on the positive relationship between the assessment of macromolecules content and infrared analysis, the extracted biosurfactant synthesized was classified as a lipopeptide. Prediction of the secondary metabolites in the non-ribosomal peptide synthetase (NRPS) clusters suggested the likelihood of the surface-active lipopeptide production in the strain’s genomic data. This is the third report of surface-active lipopeptide producers from this phylotype and the first from the polar region. The lipopeptide synthesized by ADL36 has the prospect to be an Antarctic remediation tool while furnishing a distinctive natural product for biotechnological application and research. |
format |
Article in Journal/Newspaper |
author |
Abdul Habib, Nur Muhamad Syahir Ahmad, Siti Aqlima Wan Johari, Wan Lutfi Abd. Shukor, Mohd Yunus Yasid, Nur Adeela Alias, Siti Aisyah Smykla, Jerzy Saruni, Nurul Hani Abdul Razak, Nur Syafiqah |
spellingShingle |
Abdul Habib, Nur Muhamad Syahir Ahmad, Siti Aqlima Wan Johari, Wan Lutfi Abd. Shukor, Mohd Yunus Yasid, Nur Adeela Alias, Siti Aisyah Smykla, Jerzy Saruni, Nurul Hani Abdul Razak, Nur Syafiqah Production of lipopeptide biosurfactant by a hydrocarbon-degrading antarctic Rhodococcus |
author_facet |
Abdul Habib, Nur Muhamad Syahir Ahmad, Siti Aqlima Wan Johari, Wan Lutfi Abd. Shukor, Mohd Yunus Yasid, Nur Adeela Alias, Siti Aisyah Smykla, Jerzy Saruni, Nurul Hani Abdul Razak, Nur Syafiqah |
author_sort |
Abdul Habib, Nur Muhamad Syahir |
title |
Production of lipopeptide biosurfactant by a hydrocarbon-degrading antarctic Rhodococcus |
title_short |
Production of lipopeptide biosurfactant by a hydrocarbon-degrading antarctic Rhodococcus |
title_full |
Production of lipopeptide biosurfactant by a hydrocarbon-degrading antarctic Rhodococcus |
title_fullStr |
Production of lipopeptide biosurfactant by a hydrocarbon-degrading antarctic Rhodococcus |
title_full_unstemmed |
Production of lipopeptide biosurfactant by a hydrocarbon-degrading antarctic Rhodococcus |
title_sort |
production of lipopeptide biosurfactant by a hydrocarbon-degrading antarctic rhodococcus |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
http://psasir.upm.edu.my/id/eprint/87319/ http://psasir.upm.edu.my/id/eprint/87319/1/Production%20of%20lipopeptide%20biosurfactant.pdf https://onlinelibrary.wiley.com/toc/15480569/2018/39/1 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://psasir.upm.edu.my/id/eprint/87319/1/Production%20of%20lipopeptide%20biosurfactant.pdf Abdul Habib, Nur Muhamad Syahir and Ahmad, Siti Aqlima and Wan Johari, Wan Lutfi and Abd. Shukor, Mohd Yunus and Yasid, Nur Adeela and Alias, Siti Aisyah and Smykla, Jerzy and Saruni, Nurul Hani and Abdul Razak, Nur Syafiqah (2020) Production of lipopeptide biosurfactant by a hydrocarbon-degrading antarctic Rhodococcus. International Journal of Molecular Sciences, 21 (17). pp. 1-21. ISSN 1422-0067 |
_version_ |
1766271744014811136 |