Optimization and in silico analysis of cold-adapted lipase from an antarctic pseudomonas sp strain AMS8 reaction in Triton X-100 reverse micelles

• Main Authors: Abd. Jalil, Fatin Nur Fauzi Ana, Raja Abd. Rahman, Raja Noor Zaliha, Salleh, Abu Bakar, Mohamad Ali, Mohd Shukuri
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI 2018
• Subjects: Antarctic; Triton; Antarc*
• Online Access: http://psasir.upm.edu.my/id/eprint/75154/; http://psasir.upm.edu.my/id/eprint/75154/1/Optimization%20and%20in%20silico%20analysis.pdf

A moderate yield of a purified enzyme can be achieved by using the simple technique of reverse micellar extraction (RME). RME is a liquid–liquid extraction method that uses a surfactant and an organic solvent to extract biomolecules. Instead of traditional chromatographic purification methods, which...