Large scale production of liquid wax ester by immobilized lipase

• Main Authors: Mat Radzi, Salina, Basri, Mahiran, Salleh, Abu Bakar, Ariff, Arbakariya, Mohammad, Rosfarizan, Abdul Rahman, Mohd Basyaruddin, Raja Abdul Rahman, Raja Noor Zaliha
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Japan Oil Chemists' Society 2005
• Subjects: antartic*
• Online Access: http://psasir.upm.edu.my/7057/; http://psasir.upm.edu.my/7057/1/Large%20scale%20production%20of%20liquid%20wax%20ester%20by%20immobilized%20lipase.pdf; https://www.jstage.jst.go.jp/article/jos/54/4/54_4_203/_article

Oleyl oleate, a liquid wax ester was synthesized by an immobilized Candida antartica lipase B (Novozym 435) as biocatalyst using oleic acid and oleyl alcohol. The effects of various reaction parameters were optimized to obtain a high yield of liquid wax esters. Investigation in large scale production was performed in batch mode of stirred tank reactor (STR) with one multi-bladed impeller. The optimum condition to produce liquid wax ester was, reaction time (RT); 30 min, temperature (T); 50 °C, amount of enzyme (E); 90 g (900,000 PLU), agitation speed (A) of 400 rpm, number of impeller tip (N) of 2 and molar ratio of oleyl alcohol to oleic acid (M); 2:1. Analysis of the yield showed that at optimum condition, >90% liquid wax esters were produced. The stability of Novozym 435 showed at high percentage (>80%) up to 4 cycles.