Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides
Sub-zero injuries in living tissue as a result of re-crystallization phenomenon is the major obstacle and complicates the application of cryopreserved tissues destined for transplantation. Understanding the freezing and thawing response of tissue against the sub-zero temperature would provide better...
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ftunivpmalaysia:oai:psasir.upm.edu.my:67186 2023-05-15T13:49:05+02:00 Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides Khan, Muhammad Shoaib Abubakar, Adamu Abdul Abdul Rahman, Mohd Basyaruddin Abu Bakar @ Zakaria, Md Zuki Mohamed Mustapha, Noordin Mohamad Yusof, Loqman 2015 text http://psasir.upm.edu.my/id/eprint/67186/ http://psasir.upm.edu.my/id/eprint/67186/1/6th%20PCVC%20AND%2027th%20VAM-13.pdf en eng http://psasir.upm.edu.my/id/eprint/67186/1/6th%20PCVC%20AND%2027th%20VAM-13.pdf Khan, Muhammad Shoaib and Abubakar, Adamu Abdul and Abdul Rahman, Mohd Basyaruddin and Abu Bakar @ Zakaria, Md Zuki and Mohamed Mustapha, Noordin and Mohamad Yusof, Loqman (2015) Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides. In: 6th Pan Commonwealth Veterinary Conference of the CVA and 27th Veterinary Association Malaysia Congress 2015 (PCVC6 & 27VAM), 23-27 Mar. 2015, The Royale Chulan Hotel, Kuala Lumpur, Malaysia. (pp. 473-476). Conference or Workshop Item PeerReviewed 2015 ftunivpmalaysia 2019-03-12T16:06:30Z Sub-zero injuries in living tissue as a result of re-crystallization phenomenon is the major obstacle and complicates the application of cryopreserved tissues destined for transplantation. Understanding the freezing and thawing response of tissue against the sub-zero temperature would provide better understanding in the application of the non-toxic preservation technique of living tissues. The aim of this study was to evaluate the potential of antifreeze peptides (AFP) as a cryopreservative agent for living tissue e.g. skin. To determine the effects of cryopreservation on the tissue using AFP, type 1 AFP (peptide 1 m) was used to cryopreserve skin graft in different concentrations: 0.5, 1.0 and 2.0 mg/ml at -10°C or -20°C for 24 hrs. The histological distortions effects of the tissues were measured using a scoring system to determine the extent of freezing damage incurred in cryopreserved skin tissues. It was found that relatively less microscopic tissue damages occurred at -10°C compared to -20°C at higher AFP concentration. It is concluded that skin tissue cryopreservation could maintain comparatively better microscopic tissue integrity at -10°C using 2 mg/ml concentration of AFP type 1 as the cryopreservative agent. Conference Object Antarc* Antarctic Universiti Putra Malaysia: PSAS (Perpuskataan Sultan Abuld Samad) Institutional Repository Antarctic |
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Universiti Putra Malaysia: PSAS (Perpuskataan Sultan Abuld Samad) Institutional Repository |
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ftunivpmalaysia |
language |
English |
description |
Sub-zero injuries in living tissue as a result of re-crystallization phenomenon is the major obstacle and complicates the application of cryopreserved tissues destined for transplantation. Understanding the freezing and thawing response of tissue against the sub-zero temperature would provide better understanding in the application of the non-toxic preservation technique of living tissues. The aim of this study was to evaluate the potential of antifreeze peptides (AFP) as a cryopreservative agent for living tissue e.g. skin. To determine the effects of cryopreservation on the tissue using AFP, type 1 AFP (peptide 1 m) was used to cryopreserve skin graft in different concentrations: 0.5, 1.0 and 2.0 mg/ml at -10°C or -20°C for 24 hrs. The histological distortions effects of the tissues were measured using a scoring system to determine the extent of freezing damage incurred in cryopreserved skin tissues. It was found that relatively less microscopic tissue damages occurred at -10°C compared to -20°C at higher AFP concentration. It is concluded that skin tissue cryopreservation could maintain comparatively better microscopic tissue integrity at -10°C using 2 mg/ml concentration of AFP type 1 as the cryopreservative agent. |
format |
Conference Object |
author |
Khan, Muhammad Shoaib Abubakar, Adamu Abdul Abdul Rahman, Mohd Basyaruddin Abu Bakar @ Zakaria, Md Zuki Mohamed Mustapha, Noordin Mohamad Yusof, Loqman |
spellingShingle |
Khan, Muhammad Shoaib Abubakar, Adamu Abdul Abdul Rahman, Mohd Basyaruddin Abu Bakar @ Zakaria, Md Zuki Mohamed Mustapha, Noordin Mohamad Yusof, Loqman Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides |
author_facet |
Khan, Muhammad Shoaib Abubakar, Adamu Abdul Abdul Rahman, Mohd Basyaruddin Abu Bakar @ Zakaria, Md Zuki Mohamed Mustapha, Noordin Mohamad Yusof, Loqman |
author_sort |
Khan, Muhammad Shoaib |
title |
Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides |
title_short |
Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides |
title_full |
Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides |
title_fullStr |
Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides |
title_full_unstemmed |
Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides |
title_sort |
histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides |
publishDate |
2015 |
url |
http://psasir.upm.edu.my/id/eprint/67186/ http://psasir.upm.edu.my/id/eprint/67186/1/6th%20PCVC%20AND%2027th%20VAM-13.pdf |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://psasir.upm.edu.my/id/eprint/67186/1/6th%20PCVC%20AND%2027th%20VAM-13.pdf Khan, Muhammad Shoaib and Abubakar, Adamu Abdul and Abdul Rahman, Mohd Basyaruddin and Abu Bakar @ Zakaria, Md Zuki and Mohamed Mustapha, Noordin and Mohamad Yusof, Loqman (2015) Histological assesment of low temperature epidermal tissue damage in skin graft cryopreserved with antarctic yeast isolated antifreeze peptides. In: 6th Pan Commonwealth Veterinary Conference of the CVA and 27th Veterinary Association Malaysia Congress 2015 (PCVC6 & 27VAM), 23-27 Mar. 2015, The Royale Chulan Hotel, Kuala Lumpur, Malaysia. (pp. 473-476). |
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1766250810621034496 |