Response surface methodology as a tool to study the lipase-catalyzed synthesis of betulinic acid ester

• Published in: Journal of Chemical Technology & Biotechnology
• Main Authors: Yasin, Yamin, Basri, Mahiran, Ahmad, Faujan, Salleh, Abu Bakar
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Wiley Blackwell 2008
• Subjects: Antarc*; Antarctica
• Online Access: http://psasir.upm.edu.my/6325/; http://psasir.upm.edu.my/6325/1/Response%20surface%20methodology%20as%20a%20tool%20to%20study%20the%20lipase.pdf; https://doi.org/10.1002/jctb.1858

BACKGROUND: The synthesis of betulinic acid ester using betulinic acid and oleyl alcohol catalyzed by Novozym 435 (immobilized Candida antarctica lipase) was carried out. Response surface methodology (RSM) based on a five-level, three-variable, central composite rotatable design (CCRD) was employed to evaluate the interactive effects of various parameters. The parameters were reaction time (8–16 h), temperature (20–60 ◦C) and enzyme amount (120–160 mg). RESULTS: Simultaneously increasing reaction time, temperature and amount of enzyme increased the yields of betulinic acid ester produced. CONCLUSION: The optimum conditions derived via RSM for the reaction were reaction time of 10.2 h, temperature of 53.1 ◦C and enzyme amount of 138 mg. The actual experimental yield was 48.5% under optimum conditions, which compared well with the maximum predicted value of 47.6%.