Characterization of a novel rat cytomegalovirus (RCMV) infecting placenta-uterus of Rattus rattus diardii
A new rat cytomegalovirus (RCMV) isolated from the placenta/uterus of a house rat (Rattus rattus diardii) was found to productively infect rat embryo fibroblast (REF) cells. The virus produced typical herpesvirus-like cytopathic effects characterized by a lytic infection. The well-known herpesvirus...
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Springer Wien
2003
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Online Access: | http://psasir.upm.edu.my/5803/ http://psasir.upm.edu.my/5803/1/Characterization%20of%20a%20novel%20rat%20cytomegalovirus.pdf https://doi.org/10.1007/s00705-003-0173-y |
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ftunivpmalaysia:oai:psasir.upm.edu.my:5803 2023-05-15T18:04:54+02:00 Characterization of a novel rat cytomegalovirus (RCMV) infecting placenta-uterus of Rattus rattus diardii Loh, Hwei San Mohd Lila, Mohd Azmi Lai, Kit Yee Abdul Rahman, Sheikh Omar Saad, Mohd Zamri 2003-08-18 application/pdf http://psasir.upm.edu.my/5803/ http://psasir.upm.edu.my/5803/1/Characterization%20of%20a%20novel%20rat%20cytomegalovirus.pdf https://doi.org/10.1007/s00705-003-0173-y unknown Springer Wien http://psasir.upm.edu.my/5803/1/Characterization%20of%20a%20novel%20rat%20cytomegalovirus.pdf Loh, Hwei San and Mohd Lila, Mohd Azmi and Lai, Kit Yee and Abdul Rahman, Sheikh Omar and Saad, Mohd Zamri (2003) Characterization of a novel rat cytomegalovirus (RCMV) infecting placenta-uterus of Rattus rattus diardii. Archives of Virology, 148 (12). pp. 2353-2367. ISSN 0304-8608 Article PeerReviewed 2003 ftunivpmalaysia https://doi.org/10.1007/s00705-003-0173-y 2017-06-13T15:04:24Z A new rat cytomegalovirus (RCMV) isolated from the placenta/uterus of a house rat (Rattus rattus diardii) was found to productively infect rat embryo fibroblast (REF) cells. The virus produced typical herpesvirus-like cytopathic effects characterized by a lytic infection. The well-known herpesvirus morphology was confirmed by electron microscopy. Its slow growth in cell culture indicated that the virus is belonging to subfamily Betaherpesvirinae. Electron microscopy techniques and immunohistochemistry confirmed the presence of herpesviral inclusion bodies and virus related particles in the cytoplasm and nucleus of infected cells. Hyperimmune serum against the Maastricht strain of RCMV revealed the virus identity in neutralization test, immunoperoxidase and immunofluorescence techniques. Despite typical characteristics of CMV, the viral genome is significantly different from that of Maastricht, English, UPM/Sg and UPM/Kn strains. The dissimilarities, which have not been reported before, had been confirmed by mean of restriction endonuclease analysis. The new RCMV strain, a virus that infects placenta and uterus of rats, has been named as ALL-03. Article in Journal/Newspaper Rattus rattus Universiti Putra Malaysia: PSAS (Perpuskataan Sultan Abuld Samad) Institutional Repository Archives of Virology 148 12 2353 2367 |
institution |
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Universiti Putra Malaysia: PSAS (Perpuskataan Sultan Abuld Samad) Institutional Repository |
op_collection_id |
ftunivpmalaysia |
language |
unknown |
description |
A new rat cytomegalovirus (RCMV) isolated from the placenta/uterus of a house rat (Rattus rattus diardii) was found to productively infect rat embryo fibroblast (REF) cells. The virus produced typical herpesvirus-like cytopathic effects characterized by a lytic infection. The well-known herpesvirus morphology was confirmed by electron microscopy. Its slow growth in cell culture indicated that the virus is belonging to subfamily Betaherpesvirinae. Electron microscopy techniques and immunohistochemistry confirmed the presence of herpesviral inclusion bodies and virus related particles in the cytoplasm and nucleus of infected cells. Hyperimmune serum against the Maastricht strain of RCMV revealed the virus identity in neutralization test, immunoperoxidase and immunofluorescence techniques. Despite typical characteristics of CMV, the viral genome is significantly different from that of Maastricht, English, UPM/Sg and UPM/Kn strains. The dissimilarities, which have not been reported before, had been confirmed by mean of restriction endonuclease analysis. The new RCMV strain, a virus that infects placenta and uterus of rats, has been named as ALL-03. |
format |
Article in Journal/Newspaper |
author |
Loh, Hwei San Mohd Lila, Mohd Azmi Lai, Kit Yee Abdul Rahman, Sheikh Omar Saad, Mohd Zamri |
spellingShingle |
Loh, Hwei San Mohd Lila, Mohd Azmi Lai, Kit Yee Abdul Rahman, Sheikh Omar Saad, Mohd Zamri Characterization of a novel rat cytomegalovirus (RCMV) infecting placenta-uterus of Rattus rattus diardii |
author_facet |
Loh, Hwei San Mohd Lila, Mohd Azmi Lai, Kit Yee Abdul Rahman, Sheikh Omar Saad, Mohd Zamri |
author_sort |
Loh, Hwei San |
title |
Characterization of a novel rat cytomegalovirus (RCMV) infecting placenta-uterus of Rattus rattus diardii |
title_short |
Characterization of a novel rat cytomegalovirus (RCMV) infecting placenta-uterus of Rattus rattus diardii |
title_full |
Characterization of a novel rat cytomegalovirus (RCMV) infecting placenta-uterus of Rattus rattus diardii |
title_fullStr |
Characterization of a novel rat cytomegalovirus (RCMV) infecting placenta-uterus of Rattus rattus diardii |
title_full_unstemmed |
Characterization of a novel rat cytomegalovirus (RCMV) infecting placenta-uterus of Rattus rattus diardii |
title_sort |
characterization of a novel rat cytomegalovirus (rcmv) infecting placenta-uterus of rattus rattus diardii |
publisher |
Springer Wien |
publishDate |
2003 |
url |
http://psasir.upm.edu.my/5803/ http://psasir.upm.edu.my/5803/1/Characterization%20of%20a%20novel%20rat%20cytomegalovirus.pdf https://doi.org/10.1007/s00705-003-0173-y |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
http://psasir.upm.edu.my/5803/1/Characterization%20of%20a%20novel%20rat%20cytomegalovirus.pdf Loh, Hwei San and Mohd Lila, Mohd Azmi and Lai, Kit Yee and Abdul Rahman, Sheikh Omar and Saad, Mohd Zamri (2003) Characterization of a novel rat cytomegalovirus (RCMV) infecting placenta-uterus of Rattus rattus diardii. Archives of Virology, 148 (12). pp. 2353-2367. ISSN 0304-8608 |
op_doi |
https://doi.org/10.1007/s00705-003-0173-y |
container_title |
Archives of Virology |
container_volume |
148 |
container_issue |
12 |
container_start_page |
2353 |
op_container_end_page |
2367 |
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1766176317372366848 |