Calcium-induced activity and folding of a repeat in toxin lipase from Antarctic Pseudomonas fluorescens strain AMS8
It is hypothesized that the Ca2+ ions were involved in the activity, folding and stabilization of many protein structures. Many of these proteins contain repeat in toxin (RTX) motifs. AMS8 lipase from Antarctic Pseudomonas fluorescens strain AMS8 was found to have three RTX motifs. So, this research...
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ftunivpmalaysia:oai:psasir.upm.edu.my:38185 2023-05-15T13:38:42+02:00 Calcium-induced activity and folding of a repeat in toxin lipase from Antarctic Pseudomonas fluorescens strain AMS8 Mohd Ali, Nur Shidaa Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Leow, Thean Chor Mohamad Ali, Mohd Shukuri 2020 text http://psasir.upm.edu.my/id/eprint/38185/ http://psasir.upm.edu.my/id/eprint/38185/1/38185.pdf https://www.mdpi.com/2072-6651/12/1/27 en eng MDPI http://psasir.upm.edu.my/id/eprint/38185/1/38185.pdf Mohd Ali, Nur Shidaa and Salleh, Abu Bakar and Raja Abdul Rahman, Raja Noor Zaliha and Leow, Thean Chor and Mohamad Ali, Mohd Shukuri (2020) Calcium-induced activity and folding of a repeat in toxin lipase from Antarctic Pseudomonas fluorescens strain AMS8. Toxins, 12 (1). art. no. 27. pp. 1-14. ISSN 2072-6651 Article PeerReviewed 2020 ftunivpmalaysia 2020-05-05T15:20:29Z It is hypothesized that the Ca2+ ions were involved in the activity, folding and stabilization of many protein structures. Many of these proteins contain repeat in toxin (RTX) motifs. AMS8 lipase from Antarctic Pseudomonas fluorescens strain AMS8 was found to have three RTX motifs. So, this research aimed to examine the influence of Ca2+ ion towards the activity and folding of AMS8 lipase through various biophysical characterizations. The results showed that CaCl2 increased lipase activity. The far-UV circular dichroism (CD) and Fourier-transform infrared (FTIR) analysis suggested that the secondary structure content was improved with the addition of CaCl2. Fluorescence spectroscopy analysis showed that the presence of CaCl2 increased protein folding and compactness. Dynamic light scattering (DLS) analysis suggested that AMS8 lipase became aggregated at a high concentration of CaCl2.The binding constant (Kd) value from the isothermal titration calorimetry (ITC) analysis proved that the Ca2+ ion was tightly bound to the AMS8 lipase. In conclusion, Ca2+ ions play crucial roles in the activity and folding of the AMS8 lipase. Calcium binding to RTX nonapeptide repeats sequences will induced the formation and folding of the RTX parallel β-roll motif repeat structure. Article in Journal/Newspaper Antarc* Antarctic Universiti Putra Malaysia: PSAS (Perpuskataan Sultan Abuld Samad) Institutional Repository Antarctic |
institution |
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Universiti Putra Malaysia: PSAS (Perpuskataan Sultan Abuld Samad) Institutional Repository |
op_collection_id |
ftunivpmalaysia |
language |
English |
description |
It is hypothesized that the Ca2+ ions were involved in the activity, folding and stabilization of many protein structures. Many of these proteins contain repeat in toxin (RTX) motifs. AMS8 lipase from Antarctic Pseudomonas fluorescens strain AMS8 was found to have three RTX motifs. So, this research aimed to examine the influence of Ca2+ ion towards the activity and folding of AMS8 lipase through various biophysical characterizations. The results showed that CaCl2 increased lipase activity. The far-UV circular dichroism (CD) and Fourier-transform infrared (FTIR) analysis suggested that the secondary structure content was improved with the addition of CaCl2. Fluorescence spectroscopy analysis showed that the presence of CaCl2 increased protein folding and compactness. Dynamic light scattering (DLS) analysis suggested that AMS8 lipase became aggregated at a high concentration of CaCl2.The binding constant (Kd) value from the isothermal titration calorimetry (ITC) analysis proved that the Ca2+ ion was tightly bound to the AMS8 lipase. In conclusion, Ca2+ ions play crucial roles in the activity and folding of the AMS8 lipase. Calcium binding to RTX nonapeptide repeats sequences will induced the formation and folding of the RTX parallel β-roll motif repeat structure. |
format |
Article in Journal/Newspaper |
author |
Mohd Ali, Nur Shidaa Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Leow, Thean Chor Mohamad Ali, Mohd Shukuri |
spellingShingle |
Mohd Ali, Nur Shidaa Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Leow, Thean Chor Mohamad Ali, Mohd Shukuri Calcium-induced activity and folding of a repeat in toxin lipase from Antarctic Pseudomonas fluorescens strain AMS8 |
author_facet |
Mohd Ali, Nur Shidaa Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Leow, Thean Chor Mohamad Ali, Mohd Shukuri |
author_sort |
Mohd Ali, Nur Shidaa |
title |
Calcium-induced activity and folding of a repeat in toxin lipase from Antarctic Pseudomonas fluorescens strain AMS8 |
title_short |
Calcium-induced activity and folding of a repeat in toxin lipase from Antarctic Pseudomonas fluorescens strain AMS8 |
title_full |
Calcium-induced activity and folding of a repeat in toxin lipase from Antarctic Pseudomonas fluorescens strain AMS8 |
title_fullStr |
Calcium-induced activity and folding of a repeat in toxin lipase from Antarctic Pseudomonas fluorescens strain AMS8 |
title_full_unstemmed |
Calcium-induced activity and folding of a repeat in toxin lipase from Antarctic Pseudomonas fluorescens strain AMS8 |
title_sort |
calcium-induced activity and folding of a repeat in toxin lipase from antarctic pseudomonas fluorescens strain ams8 |
publisher |
MDPI |
publishDate |
2020 |
url |
http://psasir.upm.edu.my/id/eprint/38185/ http://psasir.upm.edu.my/id/eprint/38185/1/38185.pdf https://www.mdpi.com/2072-6651/12/1/27 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://psasir.upm.edu.my/id/eprint/38185/1/38185.pdf Mohd Ali, Nur Shidaa and Salleh, Abu Bakar and Raja Abdul Rahman, Raja Noor Zaliha and Leow, Thean Chor and Mohamad Ali, Mohd Shukuri (2020) Calcium-induced activity and folding of a repeat in toxin lipase from Antarctic Pseudomonas fluorescens strain AMS8. Toxins, 12 (1). art. no. 27. pp. 1-14. ISSN 2072-6651 |
_version_ |
1766110031479046144 |