Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica

• Main Authors: Abd. Shukor , Mohd. Yunus, Hassan, N.A.A., Jusoh, A.Z., Perumal, N., Shamaan, Nor Aripin, MacCormack, W.P., Syed, Mohd. Arif
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Academy of Environmental Biology 2009
• Subjects: Antarc*; Antarctica; Antarctica Journal
• Online Access: http://psasir.upm.edu.my/15320/

A diesel-degrading bacterium from Antarctica has been isolated. The isolate was tentatively identified as Pseudomonas sp. strain DRYJ3 based on partial 16SrDNA molecular phylogeny and Biolog® GN microplate panels and Microlog® database. Growth on diesel was supported optimally by ammonium sulphate, nitrate and nitrite. The bacterium grew optimally in between 10 and 15°C, pH 7.0 and 3.5% (v/v) diesel. The biodegradation of diesel oil by the strain increased in efficiency from the second to the sixth day of incubation from 1.4 to 18.8% before levelling off on the eighth day n-alkane oxidizing and aldehyde reductase activities were detected in the crude enzyme preparation suggesting the existence of terminal n-alkane oxidizing activity in this bacterium.