EIMS Fragmentation and detection of autoxidation products of 2,6,10,14‐tetramethyl‐7‐(3‐methylpent‐4‐enyl)‐pentadec‐5‐ene in Arctic sediments

• Published in: Rapid Communications in Mass Spectrometry
• Main Authors: Rontani, J, Smik, L, Belt, ST
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2020
• Subjects: 3401 Analytical Chemistry; 34 Chemical Sciences; Arctic
• Online Access: http://hdl.handle.net/10026.1/15595; https://doi.org/10.1002/rcm.8816

12 months embargo applied <jats:sec><jats:title>Rationale</jats:title><jats:p>Some highly branched isoprenoid (HBI) alkenes are commonly used as proxies for palaeoceanographic reconstructions. However, there is a need to identify compounds that are sufficiently stable and abundant to be used as tracers of HBI oxidation in sediments. 2,6,10,14‐Tetramethyl‐7‐(3‐methylpent‐4‐enyl)‐pentadec‐5(<jats:italic>Z/E</jats:italic>)‐en‐4‐ols resulting from 2,6,10,14‐tetramethyl‐7‐(3‐methylpent‐4‐enyl)‐pentadec‐5‐ene appear to be useful for this purpose.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Comparison of electron ionization (EI) mass spectra and retention times with those of standards allowed formal identification of autoxidation products of 2,6,10,14‐tetramethyl‐7‐(3‐methylpent‐4‐enyl)‐pentadec‐5‐ene. EI‐MS fragmentations of TMS ethers of the main oxidation products (2,6,10,14‐tetramethyl‐7‐(3‐methylpent‐4‐enyl)‐pentadec‐5(<jats:italic>Z/E</jats:italic>)‐en‐4‐ols) were deduced by gas chromatography/electron ionization mass spectrometry (GC/EI‐MS), low‐energy collision‐induced dissociation tandem mass spectrometry (CID‐MS/MS) and accurate mass measurements. These compounds were then quantified in Arctic sediment samples in MS/MS multiple reaction monitoring (MRM) mode using transitions based on the main fragmentation pathways elucidated.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>2,6,10,14‐Tetramethyl‐7‐(3‐methylpent‐4‐enyl)‐pentadec‐5(<jats:italic>Z/E</jats:italic>)‐en‐4‐ols were identified after autoxidation of the HBI alkene 2,6,10,14‐tetramethyl‐7‐(3‐methylpent‐4‐enyl)‐pentadec‐5‐ene. Low‐energy CID‐MS/MS analyses and accurate mass measurements allowed the EI‐MS fragmentation pathways of their trimethylsilyl (TMS) derivatives to be elucidated. Some specific fragment ions and chromatographic retention times were also useful for ...