UV Radiation and Visible Light Induce hsp70 Gene Expression in the Antarctic Psychrophilic Ciliate Euplotes focardii
The psychrophilic ciliate Euplotes focardii inhabits the shallow marine coastal sediments of Antarctica, where, over millions of years of evolution, it has reached a strict molecular adaptation to such a constant-temperature environment (about −2°C). This long evolution at sub-zero temperatures has...
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ftunivpisairis:oai:arpi.unipi.it:11568/844625 2024-04-14T08:02:32+00:00 UV Radiation and Visible Light Induce hsp70 Gene Expression in the Antarctic Psychrophilic Ciliate Euplotes focardii Fulgentini, Lorenzo Passini, Valerio Colombetti, Giuliano Miceli, Cristina La Terza, Antonietta MARANGONI, ROBERTO Fulgentini, Lorenzo Passini, Valerio Colombetti, Giuliano Miceli, Cristina La Terza, Antonietta Marangoni, Roberto 2015 ELETTRONICO https://hdl.handle.net/11568/844625 https://doi.org/10.1007/s00248-015-0566-y eng eng info:eu-repo/semantics/altIdentifier/pmid/25666535 info:eu-repo/semantics/altIdentifier/wos/WOS:000357680900007 volume:70 issue:2 firstpage:372 lastpage:379 numberofpages:8 journal:MICROBIAL ECOLOGY https://hdl.handle.net/11568/844625 doi:10.1007/s00248-015-0566-y info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84937252408 link.springer.de/link/service/journals/00248/index.htm Euplotes focardii Gene regulation hsp70 Pigment Radiative stre UV Visible light gene activation Acclimatization Adaptation Physiological Ciliophora Euplote Light Ultraviolet Ray Ecology Evolution Behavior and Systematic Soil Science Medicine (all) info:eu-repo/semantics/article 2015 ftunivpisairis https://doi.org/10.1007/s00248-015-0566-y 2024-03-21T19:02:25Z The psychrophilic ciliate Euplotes focardii inhabits the shallow marine coastal sediments of Antarctica, where, over millions of years of evolution, it has reached a strict molecular adaptation to such a constant-temperature environment (about −2°C). This long evolution at sub-zero temperatures has made E. focardii unable to respond to heat stress with the activation of its heat shock protein (hsp) 70 genes. These genes can, however, be expressed in response to other stresses, like the oxidative one, thus indicating that the molecular adaptation has exclusively altered the heat stress signaling pathways, while it has preserved hsp70 gene activation in response to other environmental stressors. Since radiative stress has proved to be affine to oxidative stress in several organisms, we investigated the capability of UV radiation to induce hsp70 transcription. E. focardii cell cultures were exposed to several different irradiation regimes, ranging from visible only to a mixture of visible, UV-A and UV-B. The irradiation values of each spectral band have been set to be comparable with those recorded in a typical Antarctic spring. Using Northern blot analysis, we measured the expression level of hsp70 immediately after irradiation (0-h-labeled samples), 1h, and 2h from the end of the irradiation. Surprisingly, our results showed that besides UV radiation, the visible light was also able to induce hsp70 expression in E. focardii. Moreover, spectrophotometric measurements have revealed no detectable endogenous pigments in E. focardii, making it difficult to propose a possible explanation for the visible light induction of its hsp70 genes. Further research is needed to conclusively clarify this point. Article in Journal/Newspaper Antarc* Antarctic Antarctica ARPI - Archivio della Ricerca dell'Università di Pisa Antarctic The Antarctic Microbial Ecology 70 2 372 379 |
institution |
Open Polar |
collection |
ARPI - Archivio della Ricerca dell'Università di Pisa |
op_collection_id |
ftunivpisairis |
language |
English |
topic |
Euplotes focardii Gene regulation hsp70 Pigment Radiative stre UV Visible light gene activation Acclimatization Adaptation Physiological Ciliophora Euplote Light Ultraviolet Ray Ecology Evolution Behavior and Systematic Soil Science Medicine (all) |
spellingShingle |
Euplotes focardii Gene regulation hsp70 Pigment Radiative stre UV Visible light gene activation Acclimatization Adaptation Physiological Ciliophora Euplote Light Ultraviolet Ray Ecology Evolution Behavior and Systematic Soil Science Medicine (all) Fulgentini, Lorenzo Passini, Valerio Colombetti, Giuliano Miceli, Cristina La Terza, Antonietta MARANGONI, ROBERTO UV Radiation and Visible Light Induce hsp70 Gene Expression in the Antarctic Psychrophilic Ciliate Euplotes focardii |
topic_facet |
Euplotes focardii Gene regulation hsp70 Pigment Radiative stre UV Visible light gene activation Acclimatization Adaptation Physiological Ciliophora Euplote Light Ultraviolet Ray Ecology Evolution Behavior and Systematic Soil Science Medicine (all) |
description |
The psychrophilic ciliate Euplotes focardii inhabits the shallow marine coastal sediments of Antarctica, where, over millions of years of evolution, it has reached a strict molecular adaptation to such a constant-temperature environment (about −2°C). This long evolution at sub-zero temperatures has made E. focardii unable to respond to heat stress with the activation of its heat shock protein (hsp) 70 genes. These genes can, however, be expressed in response to other stresses, like the oxidative one, thus indicating that the molecular adaptation has exclusively altered the heat stress signaling pathways, while it has preserved hsp70 gene activation in response to other environmental stressors. Since radiative stress has proved to be affine to oxidative stress in several organisms, we investigated the capability of UV radiation to induce hsp70 transcription. E. focardii cell cultures were exposed to several different irradiation regimes, ranging from visible only to a mixture of visible, UV-A and UV-B. The irradiation values of each spectral band have been set to be comparable with those recorded in a typical Antarctic spring. Using Northern blot analysis, we measured the expression level of hsp70 immediately after irradiation (0-h-labeled samples), 1h, and 2h from the end of the irradiation. Surprisingly, our results showed that besides UV radiation, the visible light was also able to induce hsp70 expression in E. focardii. Moreover, spectrophotometric measurements have revealed no detectable endogenous pigments in E. focardii, making it difficult to propose a possible explanation for the visible light induction of its hsp70 genes. Further research is needed to conclusively clarify this point. |
author2 |
Fulgentini, Lorenzo Passini, Valerio Colombetti, Giuliano Miceli, Cristina La Terza, Antonietta Marangoni, Roberto |
format |
Article in Journal/Newspaper |
author |
Fulgentini, Lorenzo Passini, Valerio Colombetti, Giuliano Miceli, Cristina La Terza, Antonietta MARANGONI, ROBERTO |
author_facet |
Fulgentini, Lorenzo Passini, Valerio Colombetti, Giuliano Miceli, Cristina La Terza, Antonietta MARANGONI, ROBERTO |
author_sort |
Fulgentini, Lorenzo |
title |
UV Radiation and Visible Light Induce hsp70 Gene Expression in the Antarctic Psychrophilic Ciliate Euplotes focardii |
title_short |
UV Radiation and Visible Light Induce hsp70 Gene Expression in the Antarctic Psychrophilic Ciliate Euplotes focardii |
title_full |
UV Radiation and Visible Light Induce hsp70 Gene Expression in the Antarctic Psychrophilic Ciliate Euplotes focardii |
title_fullStr |
UV Radiation and Visible Light Induce hsp70 Gene Expression in the Antarctic Psychrophilic Ciliate Euplotes focardii |
title_full_unstemmed |
UV Radiation and Visible Light Induce hsp70 Gene Expression in the Antarctic Psychrophilic Ciliate Euplotes focardii |
title_sort |
uv radiation and visible light induce hsp70 gene expression in the antarctic psychrophilic ciliate euplotes focardii |
publishDate |
2015 |
url |
https://hdl.handle.net/11568/844625 https://doi.org/10.1007/s00248-015-0566-y |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/25666535 info:eu-repo/semantics/altIdentifier/wos/WOS:000357680900007 volume:70 issue:2 firstpage:372 lastpage:379 numberofpages:8 journal:MICROBIAL ECOLOGY https://hdl.handle.net/11568/844625 doi:10.1007/s00248-015-0566-y info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84937252408 link.springer.de/link/service/journals/00248/index.htm |
op_doi |
https://doi.org/10.1007/s00248-015-0566-y |
container_title |
Microbial Ecology |
container_volume |
70 |
container_issue |
2 |
container_start_page |
372 |
op_container_end_page |
379 |
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1796315616787824640 |