Presence and inducibility by Beta-naphthoflavone of CYP1A1, CYP1B1 and phase II enzymes in Trematomus bernacchii, an Antarctic fish
This study investigated some aspects of xenobiotic metabolism in the Nototheniidae Trematomus bernacchii, a key sentinel species for monitoring Antarctic ecosystems. After laboratory exposure to beta-naphthoflavone (beta NF), basal levels and time-course induction of CYP1A, CYP1B and CYP3A were meas...
Published in: | Aquatic Toxicology |
---|---|
Main Authors: | , , , , , , , |
Other Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2007
|
Subjects: | |
Online Access: | http://hdl.handle.net/11568/201208 https://doi.org/10.1016/j.aquatox.2007.05.010 |
id |
ftunivpisairis:oai:arpi.unipi.it:11568/201208 |
---|---|
record_format |
openpolar |
spelling |
ftunivpisairis:oai:arpi.unipi.it:11568/201208 2024-04-14T08:02:11+00:00 Presence and inducibility by Beta-naphthoflavone of CYP1A1, CYP1B1 and phase II enzymes in Trematomus bernacchii, an Antarctic fish DI BELLO D VACCARO E LONGO V REGOLI F BENEDETTI M GERVASI PG NIGRO, MARCO PRETTI, CARLO DI BELLO, D Vaccaro, E Longo, V Regoli, F Nigro, Marco Benedetti, M Gervasi, Pg Pretti, Carlo 2007 STAMPA http://hdl.handle.net/11568/201208 https://doi.org/10.1016/j.aquatox.2007.05.010 eng eng info:eu-repo/semantics/altIdentifier/pmid/PubMed ID: 17643506 info:eu-repo/semantics/altIdentifier/wos/000249165800003 volume:84 issue:1 firstpage:19 lastpage:26 numberofpages:8 journal:AQUATIC TOXICOLOGY http://hdl.handle.net/11568/201208 doi:10.1016/j.aquatox.2007.05.010 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-34447625702 info:eu-repo/semantics/article 2007 ftunivpisairis https://doi.org/10.1016/j.aquatox.2007.05.010 2024-03-21T18:40:25Z This study investigated some aspects of xenobiotic metabolism in the Nototheniidae Trematomus bernacchii, a key sentinel species for monitoring Antarctic ecosystems. After laboratory exposure to beta-naphthoflavone (beta NF), basal levels and time-course induction of CYP1A, CYP1B and CYP3A were measured as enzymatic activities, immunoreactive protein content and mRNA expression in liver, gills, intestine and heart. Additional analyses in the liver included enzymatic activities of testosterone hydroxylase, (omega)- and (omega - 1)-lauric acid hydroxylase and some phase II enzymes related to the AhR battery genes, DT-diaphorase, glutathione S-transferases and UDP-glucuronyl transferases. Responsiveness of hepatic CYP1A1 after exposure to PNF demonstrated an higher sensitivity of MEROD than EROD activity and long lasting expression of mRNA still induced after 20 days from the treatment. Testosterone metabolism, oxidation of lauric acid and activities of phase II enzymes were not affected by PNF indicating that their modulation is not mediated by Ah receptor. Induction of CYP1A was more limited in gills and absent in intestine and heart. The first nucleotide sequence for CYP1B1 in an Antarctic fish has been obtained, revealing a homology of 89% and 72% respectively to CYP1B1 of plaice and CYP1B2 of carp. Constitutive expression of CYP1B1 was restricted to gills where it was also induced by beta NE Obtained results represent an additional contribution to the ecotoxicological characterization of T bernacchii and further support the use of biomarkers for early detection of chemical pollution in Antarctica. (c) 2007 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Antarc* Antarctic Antarctica ARPI - Archivio della Ricerca dell'Università di Pisa Antarctic Aquatic Toxicology 84 1 19 26 |
institution |
Open Polar |
collection |
ARPI - Archivio della Ricerca dell'Università di Pisa |
op_collection_id |
ftunivpisairis |
language |
English |
description |
This study investigated some aspects of xenobiotic metabolism in the Nototheniidae Trematomus bernacchii, a key sentinel species for monitoring Antarctic ecosystems. After laboratory exposure to beta-naphthoflavone (beta NF), basal levels and time-course induction of CYP1A, CYP1B and CYP3A were measured as enzymatic activities, immunoreactive protein content and mRNA expression in liver, gills, intestine and heart. Additional analyses in the liver included enzymatic activities of testosterone hydroxylase, (omega)- and (omega - 1)-lauric acid hydroxylase and some phase II enzymes related to the AhR battery genes, DT-diaphorase, glutathione S-transferases and UDP-glucuronyl transferases. Responsiveness of hepatic CYP1A1 after exposure to PNF demonstrated an higher sensitivity of MEROD than EROD activity and long lasting expression of mRNA still induced after 20 days from the treatment. Testosterone metabolism, oxidation of lauric acid and activities of phase II enzymes were not affected by PNF indicating that their modulation is not mediated by Ah receptor. Induction of CYP1A was more limited in gills and absent in intestine and heart. The first nucleotide sequence for CYP1B1 in an Antarctic fish has been obtained, revealing a homology of 89% and 72% respectively to CYP1B1 of plaice and CYP1B2 of carp. Constitutive expression of CYP1B1 was restricted to gills where it was also induced by beta NE Obtained results represent an additional contribution to the ecotoxicological characterization of T bernacchii and further support the use of biomarkers for early detection of chemical pollution in Antarctica. (c) 2007 Elsevier B.V. All rights reserved. |
author2 |
DI BELLO, D Vaccaro, E Longo, V Regoli, F Nigro, Marco Benedetti, M Gervasi, Pg Pretti, Carlo |
format |
Article in Journal/Newspaper |
author |
DI BELLO D VACCARO E LONGO V REGOLI F BENEDETTI M GERVASI PG NIGRO, MARCO PRETTI, CARLO |
spellingShingle |
DI BELLO D VACCARO E LONGO V REGOLI F BENEDETTI M GERVASI PG NIGRO, MARCO PRETTI, CARLO Presence and inducibility by Beta-naphthoflavone of CYP1A1, CYP1B1 and phase II enzymes in Trematomus bernacchii, an Antarctic fish |
author_facet |
DI BELLO D VACCARO E LONGO V REGOLI F BENEDETTI M GERVASI PG NIGRO, MARCO PRETTI, CARLO |
author_sort |
DI BELLO D |
title |
Presence and inducibility by Beta-naphthoflavone of CYP1A1, CYP1B1 and phase II enzymes in Trematomus bernacchii, an Antarctic fish |
title_short |
Presence and inducibility by Beta-naphthoflavone of CYP1A1, CYP1B1 and phase II enzymes in Trematomus bernacchii, an Antarctic fish |
title_full |
Presence and inducibility by Beta-naphthoflavone of CYP1A1, CYP1B1 and phase II enzymes in Trematomus bernacchii, an Antarctic fish |
title_fullStr |
Presence and inducibility by Beta-naphthoflavone of CYP1A1, CYP1B1 and phase II enzymes in Trematomus bernacchii, an Antarctic fish |
title_full_unstemmed |
Presence and inducibility by Beta-naphthoflavone of CYP1A1, CYP1B1 and phase II enzymes in Trematomus bernacchii, an Antarctic fish |
title_sort |
presence and inducibility by beta-naphthoflavone of cyp1a1, cyp1b1 and phase ii enzymes in trematomus bernacchii, an antarctic fish |
publishDate |
2007 |
url |
http://hdl.handle.net/11568/201208 https://doi.org/10.1016/j.aquatox.2007.05.010 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/PubMed ID: 17643506 info:eu-repo/semantics/altIdentifier/wos/000249165800003 volume:84 issue:1 firstpage:19 lastpage:26 numberofpages:8 journal:AQUATIC TOXICOLOGY http://hdl.handle.net/11568/201208 doi:10.1016/j.aquatox.2007.05.010 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-34447625702 |
op_doi |
https://doi.org/10.1016/j.aquatox.2007.05.010 |
container_title |
Aquatic Toxicology |
container_volume |
84 |
container_issue |
1 |
container_start_page |
19 |
op_container_end_page |
26 |
_version_ |
1796313246433542144 |