Multiplex conventional and real-time PCR for fish species identification of Bianchetto (juvenile form of Sardina pilchardus), Rossetto (Aphia minuta), and Icefish in fresh, marinated and cooked products
A conventional and a realtime multiplex PCR were developed to detect fraudulent substitutions of Bianchetto (juvenile form of Sardina philcardus) and Rossetto (Aphia minuta) with Icefish (Neosalanx spp.), which show similar morphological characteristics. Since it is the major by-catch species, Engra...
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Online Access: | https://hdl.handle.net/11568/199707 https://doi.org/10.1016/j.foodchem.2011.12.076 https://www.sciencedirect.com/science/article/pii/S0308814612000027 |
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ftunivpisairis:oai:arpi.unipi.it:11568/199707 2024-04-14T08:13:20+00:00 Multiplex conventional and real-time PCR for fish species identification of Bianchetto (juvenile form of Sardina pilchardus), Rossetto (Aphia minuta), and Icefish in fresh, marinated and cooked products ARMANI, ANDREA CASTIGLIEGO, LORENZO Tinacci L GIANFALDONI, DANIELA GUIDI, ALESSANDRA Armani, Andrea Castigliego, Lorenzo Tinacci, L Gianfaldoni, Daniela Guidi, Alessandra 2012 https://hdl.handle.net/11568/199707 https://doi.org/10.1016/j.foodchem.2011.12.076 https://www.sciencedirect.com/science/article/pii/S0308814612000027 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000301826400026 volume:133 firstpage:184 lastpage:192 numberofpages:9 journal:FOOD CHEMISTRY https://hdl.handle.net/11568/199707 doi:10.1016/j.foodchem.2011.12.076 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84857065620 https://www.sciencedirect.com/science/article/pii/S0308814612000027 Sardina pilchardus Aphia minuta Engraulis encrasicolus Icefish Multiplex PCR Cytochrome b info:eu-repo/semantics/article 2012 ftunivpisairis https://doi.org/10.1016/j.foodchem.2011.12.076 2024-03-21T18:39:42Z A conventional and a realtime multiplex PCR were developed to detect fraudulent substitutions of Bianchetto (juvenile form of Sardina philcardus) and Rossetto (Aphia minuta) with Icefish (Neosalanx spp.), which show similar morphological characteristics. Since it is the major by-catch species, Engraulis encrasicolus was also included in the analytical procedure. A common reverse primer and forward species-specific primer were designed on the mitochondrial cytochrome b gene to amplify sequences of different lengths by conventional PCR. Specific peaks were therefore also provided after melting temperature analysis in real-time PCR, thus enabling each species to be clearly differentiated. The two PCR methods were validated on fresh and processed products after preparing four typical dishes: two marinades (from raw or lightly boiled fish), a pasta sauce, and batter-fried fish cakes. All samples were correctly identified, although there was some DNA degradation after processing. Article in Journal/Newspaper Icefish ARPI - Archivio della Ricerca dell'Università di Pisa Food Chemistry 133 1 184 192 |
institution |
Open Polar |
collection |
ARPI - Archivio della Ricerca dell'Università di Pisa |
op_collection_id |
ftunivpisairis |
language |
English |
topic |
Sardina pilchardus Aphia minuta Engraulis encrasicolus Icefish Multiplex PCR Cytochrome b |
spellingShingle |
Sardina pilchardus Aphia minuta Engraulis encrasicolus Icefish Multiplex PCR Cytochrome b ARMANI, ANDREA CASTIGLIEGO, LORENZO Tinacci L GIANFALDONI, DANIELA GUIDI, ALESSANDRA Multiplex conventional and real-time PCR for fish species identification of Bianchetto (juvenile form of Sardina pilchardus), Rossetto (Aphia minuta), and Icefish in fresh, marinated and cooked products |
topic_facet |
Sardina pilchardus Aphia minuta Engraulis encrasicolus Icefish Multiplex PCR Cytochrome b |
description |
A conventional and a realtime multiplex PCR were developed to detect fraudulent substitutions of Bianchetto (juvenile form of Sardina philcardus) and Rossetto (Aphia minuta) with Icefish (Neosalanx spp.), which show similar morphological characteristics. Since it is the major by-catch species, Engraulis encrasicolus was also included in the analytical procedure. A common reverse primer and forward species-specific primer were designed on the mitochondrial cytochrome b gene to amplify sequences of different lengths by conventional PCR. Specific peaks were therefore also provided after melting temperature analysis in real-time PCR, thus enabling each species to be clearly differentiated. The two PCR methods were validated on fresh and processed products after preparing four typical dishes: two marinades (from raw or lightly boiled fish), a pasta sauce, and batter-fried fish cakes. All samples were correctly identified, although there was some DNA degradation after processing. |
author2 |
Armani, Andrea Castigliego, Lorenzo Tinacci, L Gianfaldoni, Daniela Guidi, Alessandra |
format |
Article in Journal/Newspaper |
author |
ARMANI, ANDREA CASTIGLIEGO, LORENZO Tinacci L GIANFALDONI, DANIELA GUIDI, ALESSANDRA |
author_facet |
ARMANI, ANDREA CASTIGLIEGO, LORENZO Tinacci L GIANFALDONI, DANIELA GUIDI, ALESSANDRA |
author_sort |
ARMANI, ANDREA |
title |
Multiplex conventional and real-time PCR for fish species identification of Bianchetto (juvenile form of Sardina pilchardus), Rossetto (Aphia minuta), and Icefish in fresh, marinated and cooked products |
title_short |
Multiplex conventional and real-time PCR for fish species identification of Bianchetto (juvenile form of Sardina pilchardus), Rossetto (Aphia minuta), and Icefish in fresh, marinated and cooked products |
title_full |
Multiplex conventional and real-time PCR for fish species identification of Bianchetto (juvenile form of Sardina pilchardus), Rossetto (Aphia minuta), and Icefish in fresh, marinated and cooked products |
title_fullStr |
Multiplex conventional and real-time PCR for fish species identification of Bianchetto (juvenile form of Sardina pilchardus), Rossetto (Aphia minuta), and Icefish in fresh, marinated and cooked products |
title_full_unstemmed |
Multiplex conventional and real-time PCR for fish species identification of Bianchetto (juvenile form of Sardina pilchardus), Rossetto (Aphia minuta), and Icefish in fresh, marinated and cooked products |
title_sort |
multiplex conventional and real-time pcr for fish species identification of bianchetto (juvenile form of sardina pilchardus), rossetto (aphia minuta), and icefish in fresh, marinated and cooked products |
publishDate |
2012 |
url |
https://hdl.handle.net/11568/199707 https://doi.org/10.1016/j.foodchem.2011.12.076 https://www.sciencedirect.com/science/article/pii/S0308814612000027 |
genre |
Icefish |
genre_facet |
Icefish |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000301826400026 volume:133 firstpage:184 lastpage:192 numberofpages:9 journal:FOOD CHEMISTRY https://hdl.handle.net/11568/199707 doi:10.1016/j.foodchem.2011.12.076 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84857065620 https://www.sciencedirect.com/science/article/pii/S0308814612000027 |
op_doi |
https://doi.org/10.1016/j.foodchem.2011.12.076 |
container_title |
Food Chemistry |
container_volume |
133 |
container_issue |
1 |
container_start_page |
184 |
op_container_end_page |
192 |
_version_ |
1796311293976641536 |