Induction of DNA strand breakage and apoptosis in the eel Anguilla anguilla
The ability of benzo[a]pyrene, Aroclor 1254, 2-3-7-8-tetrachlorodibenzo-p-dioxin and beta-naphthoflavone to induce DNA strand breaks (SB) and apoptosis in erythrocytes of the European eel (Anguilla anguilla) was investigated following by in vivo exposure. DNA damage was evaluated by the Comet assay,...
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Online Access: | http://hdl.handle.net/11568/178046 https://doi.org/10.1016/S0141-1136(02)00178-2 |
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ftunivpisairis:oai:arpi.unipi.it:11568/178046 2024-04-21T07:45:31+00:00 Induction of DNA strand breakage and apoptosis in the eel Anguilla anguilla NIGRO, MARCO FRENZILLI, GIADA SCARCELLI V. GORBI S. REGOLI F. Nigro, Marco Frenzilli, Giada Scarcelli, V. Gorbi, S. Regoli, F. 2002 http://hdl.handle.net/11568/178046 https://doi.org/10.1016/S0141-1136(02)00178-2 eng eng info:eu-repo/semantics/altIdentifier/pmid/12408611 info:eu-repo/semantics/altIdentifier/wos/000178360100056 volume:54 firstpage:517 lastpage:520 numberofpages:4 journal:MARINE ENVIRONMENTAL RESEARCH http://hdl.handle.net/11568/178046 doi:10.1016/S0141-1136(02)00178-2 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0036752240 info:eu-repo/semantics/article 2002 ftunivpisairis https://doi.org/10.1016/S0141-1136(02)00178-2 2024-03-28T01:26:37Z The ability of benzo[a]pyrene, Aroclor 1254, 2-3-7-8-tetrachlorodibenzo-p-dioxin and beta-naphthoflavone to induce DNA strand breaks (SB) and apoptosis in erythrocytes of the European eel (Anguilla anguilla) was investigated following by in vivo exposure. DNA damage was evaluated by the Comet assay, while the diffusion assay was used to investigate the induction of apoptosis 7 days after a single intraperitoneal administration. 2-3-7-8-Tetrachlorodibenzo-p-dioxin induced the highest genotoxic effect, followed by benzo[a]pyrene, while the other two substances had limited effects. A significant induction of apoptosis was observed at the highest doses after exposure to benzo[a]pyrene, when DNA damage was also elevated. The occurrence of apoptotic cells after exposure to Aroclor, 2-3-7-8-tetrachlorodibenzo-p-dioxin and beta-naphthoflavone was quite variable and did not show clear dose-related responses. The role of oxidative stress in mediating DNA damage was also discussed. Article in Journal/Newspaper Anguilla anguilla ARPI - Archivio della Ricerca dell'Università di Pisa Marine Environmental Research 54 3-5 517 520 |
institution |
Open Polar |
collection |
ARPI - Archivio della Ricerca dell'Università di Pisa |
op_collection_id |
ftunivpisairis |
language |
English |
description |
The ability of benzo[a]pyrene, Aroclor 1254, 2-3-7-8-tetrachlorodibenzo-p-dioxin and beta-naphthoflavone to induce DNA strand breaks (SB) and apoptosis in erythrocytes of the European eel (Anguilla anguilla) was investigated following by in vivo exposure. DNA damage was evaluated by the Comet assay, while the diffusion assay was used to investigate the induction of apoptosis 7 days after a single intraperitoneal administration. 2-3-7-8-Tetrachlorodibenzo-p-dioxin induced the highest genotoxic effect, followed by benzo[a]pyrene, while the other two substances had limited effects. A significant induction of apoptosis was observed at the highest doses after exposure to benzo[a]pyrene, when DNA damage was also elevated. The occurrence of apoptotic cells after exposure to Aroclor, 2-3-7-8-tetrachlorodibenzo-p-dioxin and beta-naphthoflavone was quite variable and did not show clear dose-related responses. The role of oxidative stress in mediating DNA damage was also discussed. |
author2 |
Nigro, Marco Frenzilli, Giada Scarcelli, V. Gorbi, S. Regoli, F. |
format |
Article in Journal/Newspaper |
author |
NIGRO, MARCO FRENZILLI, GIADA SCARCELLI V. GORBI S. REGOLI F. |
spellingShingle |
NIGRO, MARCO FRENZILLI, GIADA SCARCELLI V. GORBI S. REGOLI F. Induction of DNA strand breakage and apoptosis in the eel Anguilla anguilla |
author_facet |
NIGRO, MARCO FRENZILLI, GIADA SCARCELLI V. GORBI S. REGOLI F. |
author_sort |
NIGRO, MARCO |
title |
Induction of DNA strand breakage and apoptosis in the eel Anguilla anguilla |
title_short |
Induction of DNA strand breakage and apoptosis in the eel Anguilla anguilla |
title_full |
Induction of DNA strand breakage and apoptosis in the eel Anguilla anguilla |
title_fullStr |
Induction of DNA strand breakage and apoptosis in the eel Anguilla anguilla |
title_full_unstemmed |
Induction of DNA strand breakage and apoptosis in the eel Anguilla anguilla |
title_sort |
induction of dna strand breakage and apoptosis in the eel anguilla anguilla |
publishDate |
2002 |
url |
http://hdl.handle.net/11568/178046 https://doi.org/10.1016/S0141-1136(02)00178-2 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/12408611 info:eu-repo/semantics/altIdentifier/wos/000178360100056 volume:54 firstpage:517 lastpage:520 numberofpages:4 journal:MARINE ENVIRONMENTAL RESEARCH http://hdl.handle.net/11568/178046 doi:10.1016/S0141-1136(02)00178-2 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0036752240 |
op_doi |
https://doi.org/10.1016/S0141-1136(02)00178-2 |
container_title |
Marine Environmental Research |
container_volume |
54 |
container_issue |
3-5 |
container_start_page |
517 |
op_container_end_page |
520 |
_version_ |
1796940457195864064 |