Interactions between trace metals (Cu, Hg, Ni, Pb) and 2,3,7,8- tetrachlorodibenzo-p-dioxin in the antarctic fish trematomus bernacchii: oxidative effects on biotransformation pathway
Biological effects of chemical mixtures are an emerging issue when using biomarkers in field conditions, because synergistic or inhibitory interactions, cascade, and indirect mechanisms can both enhance or suppress responses to specific classes of pollutants. The Antarctic rock cod (Trematomus berna...
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Online Access: | http://hdl.handle.net/11568/134825 https://doi.org/10.1897/08-066.1 |
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ftunivpisairis:oai:arpi.unipi.it:11568/134825 2024-02-27T08:35:09+00:00 Interactions between trace metals (Cu, Hg, Ni, Pb) and 2,3,7,8- tetrachlorodibenzo-p-dioxin in the antarctic fish trematomus bernacchii: oxidative effects on biotransformation pathway BENEDETTI, M FATTORINI, D MARTUCCIO, G REGOLI, F. NIGRO, MARCO Benedetti, M Fattorini, D Martuccio, G Nigro, Marco Regoli, F. 2009 STAMPA http://hdl.handle.net/11568/134825 https://doi.org/10.1897/08-066.1 eng eng info:eu-repo/semantics/altIdentifier/pmid/PubMed ID: 19006418 info:eu-repo/semantics/altIdentifier/wos/WOS:000264044700015 volume:28 issue:4 firstpage:818 lastpage:825 numberofpages:8 journal:ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY http://hdl.handle.net/11568/134825 doi:10.1897/08-066.1 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-64849103104 info:eu-repo/semantics/article 2009 ftunivpisairis https://doi.org/10.1897/08-066.1 2024-01-31T17:42:22Z Biological effects of chemical mixtures are an emerging issue when using biomarkers in field conditions, because synergistic or inhibitory interactions, cascade, and indirect mechanisms can both enhance or suppress responses to specific classes of pollutants. The Antarctic rock cod (Trematomus bernacchii) was exposed to various trace metals (Cu, Hg, Ni, and Pb) and to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), dosed alone or in combination, to generate hypotheses regarding the mechanisms of action at the cellular level. Concentrations of metals significantly increased in liver of T. bernacchii without significant differences between organisms exposed only to the elements or cotreated with TCDD. The marked induction of cytochrome P450 (CYP) by TCDD was greatly suppressed by cotreatment with Cu at both catalytic and protein expression levels, whereas no significant effects were caused by coexposures with Hg, Ni, and Pb. The oxidative status was measured by integrating individual antioxidants with the total oxyradical scavenging capacity toward peroxyl radicals (ROO center dot) and hydroxyl radicals (center dot OH). Synergistic oxidative effects appeared in fish coexposed to Cu and TCDD, suggesting that biotransformation efficiency is down-regulated by higher levels of hydrogen peroxide, hydroxyl radicals, and/or mechanisms limiting availability of heme groups. Major oxidative perturbations also were observed in organisms coexposed to TCDD and Hg; in these organisms, however, the marked increase of glutathione content could explain the absence of oxidative inhibition of the CYP system. More limited oxidative effects were caused by Ni and Pb, and the contemporary absence of inhibitory effects on CYP would further confirm the importance of pro-oxidant mechanisms in modulating the biotransformation pathway. The overall results indicate that a wide battery of biomarkers is necessary to assess the impact of chemical mixtures in field conditions Article in Journal/Newspaper Antarc* Antarctic ARPI - Archivio della Ricerca dell'Università di Pisa Antarctic The Antarctic Environmental Toxicology and Chemistry 28 4 818 |
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Open Polar |
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ARPI - Archivio della Ricerca dell'Università di Pisa |
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ftunivpisairis |
language |
English |
description |
Biological effects of chemical mixtures are an emerging issue when using biomarkers in field conditions, because synergistic or inhibitory interactions, cascade, and indirect mechanisms can both enhance or suppress responses to specific classes of pollutants. The Antarctic rock cod (Trematomus bernacchii) was exposed to various trace metals (Cu, Hg, Ni, and Pb) and to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), dosed alone or in combination, to generate hypotheses regarding the mechanisms of action at the cellular level. Concentrations of metals significantly increased in liver of T. bernacchii without significant differences between organisms exposed only to the elements or cotreated with TCDD. The marked induction of cytochrome P450 (CYP) by TCDD was greatly suppressed by cotreatment with Cu at both catalytic and protein expression levels, whereas no significant effects were caused by coexposures with Hg, Ni, and Pb. The oxidative status was measured by integrating individual antioxidants with the total oxyradical scavenging capacity toward peroxyl radicals (ROO center dot) and hydroxyl radicals (center dot OH). Synergistic oxidative effects appeared in fish coexposed to Cu and TCDD, suggesting that biotransformation efficiency is down-regulated by higher levels of hydrogen peroxide, hydroxyl radicals, and/or mechanisms limiting availability of heme groups. Major oxidative perturbations also were observed in organisms coexposed to TCDD and Hg; in these organisms, however, the marked increase of glutathione content could explain the absence of oxidative inhibition of the CYP system. More limited oxidative effects were caused by Ni and Pb, and the contemporary absence of inhibitory effects on CYP would further confirm the importance of pro-oxidant mechanisms in modulating the biotransformation pathway. The overall results indicate that a wide battery of biomarkers is necessary to assess the impact of chemical mixtures in field conditions |
author2 |
Benedetti, M Fattorini, D Martuccio, G Nigro, Marco Regoli, F. |
format |
Article in Journal/Newspaper |
author |
BENEDETTI, M FATTORINI, D MARTUCCIO, G REGOLI, F. NIGRO, MARCO |
spellingShingle |
BENEDETTI, M FATTORINI, D MARTUCCIO, G REGOLI, F. NIGRO, MARCO Interactions between trace metals (Cu, Hg, Ni, Pb) and 2,3,7,8- tetrachlorodibenzo-p-dioxin in the antarctic fish trematomus bernacchii: oxidative effects on biotransformation pathway |
author_facet |
BENEDETTI, M FATTORINI, D MARTUCCIO, G REGOLI, F. NIGRO, MARCO |
author_sort |
BENEDETTI, M |
title |
Interactions between trace metals (Cu, Hg, Ni, Pb) and 2,3,7,8- tetrachlorodibenzo-p-dioxin in the antarctic fish trematomus bernacchii: oxidative effects on biotransformation pathway |
title_short |
Interactions between trace metals (Cu, Hg, Ni, Pb) and 2,3,7,8- tetrachlorodibenzo-p-dioxin in the antarctic fish trematomus bernacchii: oxidative effects on biotransformation pathway |
title_full |
Interactions between trace metals (Cu, Hg, Ni, Pb) and 2,3,7,8- tetrachlorodibenzo-p-dioxin in the antarctic fish trematomus bernacchii: oxidative effects on biotransformation pathway |
title_fullStr |
Interactions between trace metals (Cu, Hg, Ni, Pb) and 2,3,7,8- tetrachlorodibenzo-p-dioxin in the antarctic fish trematomus bernacchii: oxidative effects on biotransformation pathway |
title_full_unstemmed |
Interactions between trace metals (Cu, Hg, Ni, Pb) and 2,3,7,8- tetrachlorodibenzo-p-dioxin in the antarctic fish trematomus bernacchii: oxidative effects on biotransformation pathway |
title_sort |
interactions between trace metals (cu, hg, ni, pb) and 2,3,7,8- tetrachlorodibenzo-p-dioxin in the antarctic fish trematomus bernacchii: oxidative effects on biotransformation pathway |
publishDate |
2009 |
url |
http://hdl.handle.net/11568/134825 https://doi.org/10.1897/08-066.1 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/PubMed ID: 19006418 info:eu-repo/semantics/altIdentifier/wos/WOS:000264044700015 volume:28 issue:4 firstpage:818 lastpage:825 numberofpages:8 journal:ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY http://hdl.handle.net/11568/134825 doi:10.1897/08-066.1 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-64849103104 |
op_doi |
https://doi.org/10.1897/08-066.1 |
container_title |
Environmental Toxicology and Chemistry |
container_volume |
28 |
container_issue |
4 |
container_start_page |
818 |
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1792041625933840384 |