Mutual benefits from the symbiotic coexistence between bipolar Euplotes cells and Parafrancisella bacteria
Ciliates are common carriers of cytoplasmic bacteria, however little is known about the molecular basis of the symbiotic relationships with their host. Working on interbreeding bipolar (Arctic and Antarctic) populations of the ciliate Euplotes nobilii, members of these populations were found to stab...
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Università di Vienna (Austria)
2023
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ftunivpisairis:oai:arpi.unipi.it:11568/1219856 2024-04-21T07:46:26+00:00 Mutual benefits from the symbiotic coexistence between bipolar Euplotes cells and Parafrancisella bacteria Vallesi Adriana Candelori Annalisa Di Giuseppe Graziano Villalobo Eduardo Sjödin Andreas Università di Vienna (Austria) Vallesi, Adriana Candelori, Annalisa DI GIUSEPPE, Graziano Villalobo, Eduardo Sjödin, Andreas 2023 STAMPA https://hdl.handle.net/11568/1219856 eng eng Università di Vienna (Austria) country:AUT place:Vienna ispartofbook:ECOP-ISOP joint meeting "The Century of Protists" ECOP-ISOP joint meeting "The Century of Protists" volume:1 issue:1 firstpage:369 numberofpages:1 alleditors:Università di Vienna (Austria) https://hdl.handle.net/11568/1219856 info:eu-repo/semantics/conferenceObject 2023 ftunivpisairis 2024-03-28T01:21:38Z Ciliates are common carriers of cytoplasmic bacteria, however little is known about the molecular basis of the symbiotic relationships with their host. Working on interbreeding bipolar (Arctic and Antarctic) populations of the ciliate Euplotes nobilii, members of these populations were found to stably host Parafrancisella γ-proteobacteria. These bacteria (originally isolated from an Antarctic population of another Euplotes species, E. petzi) are phylogenetically related to pathogenic Francisella species which are well known for their capacity to colonize eukaryotic cells, causing animal diseases known as francisellosis. The finding that Parafrancisella/Euplotes associations are common in polar marine environments suggested that both microbial partners benefit from their stable partnership. To inquire into mutual advantages, we carried out a genomic analysis of E. nobilii and its Parafrancisella symbionts. In the E. nobilii genome, no gene was detected encoding methionine sulfoxide reductase of type A (MsrA), an enzyme which is essential to face damages from oxidative stress imposed by the high (saturated) oxygen concentrations of the polar sea waters. At the same time, the Parafrancisella genome revealed genes encoding MsrA sequences endowed with a N-terminal signal peptide for the secretion into the host’s cytoplasm, and the effectiveness of this secretion was further supported by the identification of a complete gene set coding for the so-called ‘Type VI Secretion System’ that many Gram-negative bacteria use to transfer their proteins into target cells. On the other side, in the Parafrancisella genome no gene encoding enzymes involved in the biosynthetic pathways of cysteine, lysine, methionine, and threonine was detected, implying that Parafrancisella cells rely on their E. nobilii host to obtain these four essential amino acids. Conference Object Antarc* Antarctic ARPI - Archivio della Ricerca dell'Università di Pisa |
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Open Polar |
collection |
ARPI - Archivio della Ricerca dell'Università di Pisa |
op_collection_id |
ftunivpisairis |
language |
English |
description |
Ciliates are common carriers of cytoplasmic bacteria, however little is known about the molecular basis of the symbiotic relationships with their host. Working on interbreeding bipolar (Arctic and Antarctic) populations of the ciliate Euplotes nobilii, members of these populations were found to stably host Parafrancisella γ-proteobacteria. These bacteria (originally isolated from an Antarctic population of another Euplotes species, E. petzi) are phylogenetically related to pathogenic Francisella species which are well known for their capacity to colonize eukaryotic cells, causing animal diseases known as francisellosis. The finding that Parafrancisella/Euplotes associations are common in polar marine environments suggested that both microbial partners benefit from their stable partnership. To inquire into mutual advantages, we carried out a genomic analysis of E. nobilii and its Parafrancisella symbionts. In the E. nobilii genome, no gene was detected encoding methionine sulfoxide reductase of type A (MsrA), an enzyme which is essential to face damages from oxidative stress imposed by the high (saturated) oxygen concentrations of the polar sea waters. At the same time, the Parafrancisella genome revealed genes encoding MsrA sequences endowed with a N-terminal signal peptide for the secretion into the host’s cytoplasm, and the effectiveness of this secretion was further supported by the identification of a complete gene set coding for the so-called ‘Type VI Secretion System’ that many Gram-negative bacteria use to transfer their proteins into target cells. On the other side, in the Parafrancisella genome no gene encoding enzymes involved in the biosynthetic pathways of cysteine, lysine, methionine, and threonine was detected, implying that Parafrancisella cells rely on their E. nobilii host to obtain these four essential amino acids. |
author2 |
Università di Vienna (Austria) Vallesi, Adriana Candelori, Annalisa DI GIUSEPPE, Graziano Villalobo, Eduardo Sjödin, Andreas |
format |
Conference Object |
author |
Vallesi Adriana Candelori Annalisa Di Giuseppe Graziano Villalobo Eduardo Sjödin Andreas |
spellingShingle |
Vallesi Adriana Candelori Annalisa Di Giuseppe Graziano Villalobo Eduardo Sjödin Andreas Mutual benefits from the symbiotic coexistence between bipolar Euplotes cells and Parafrancisella bacteria |
author_facet |
Vallesi Adriana Candelori Annalisa Di Giuseppe Graziano Villalobo Eduardo Sjödin Andreas |
author_sort |
Vallesi Adriana |
title |
Mutual benefits from the symbiotic coexistence between bipolar Euplotes cells and Parafrancisella bacteria |
title_short |
Mutual benefits from the symbiotic coexistence between bipolar Euplotes cells and Parafrancisella bacteria |
title_full |
Mutual benefits from the symbiotic coexistence between bipolar Euplotes cells and Parafrancisella bacteria |
title_fullStr |
Mutual benefits from the symbiotic coexistence between bipolar Euplotes cells and Parafrancisella bacteria |
title_full_unstemmed |
Mutual benefits from the symbiotic coexistence between bipolar Euplotes cells and Parafrancisella bacteria |
title_sort |
mutual benefits from the symbiotic coexistence between bipolar euplotes cells and parafrancisella bacteria |
publisher |
Università di Vienna (Austria) |
publishDate |
2023 |
url |
https://hdl.handle.net/11568/1219856 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
ispartofbook:ECOP-ISOP joint meeting "The Century of Protists" ECOP-ISOP joint meeting "The Century of Protists" volume:1 issue:1 firstpage:369 numberofpages:1 alleditors:Università di Vienna (Austria) https://hdl.handle.net/11568/1219856 |
_version_ |
1796943546141376512 |