Biocatalyzed Hydrolysis of Residual Oils and Proteins from Flax and Camelina Oilseed Press Cakes Using Lipase and Protease
In this study, a PoC (Proof of Concept) of a possible biomass valorization of flax and camelina oilseed press cakes was presented. Biocatalyzed hydrolysis of residual oils and proteins extracted from these wastes was studied. The biotransformation of oils was performed using commercial immobilized l...
Published in: | Catalysis Research |
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Online Access: | https://hdl.handle.net/11568/1166078 https://doi.org/10.21926/cr.2204041 |
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ftunivpisairis:oai:arpi.unipi.it:11568/1166078 2024-06-23T07:47:48+00:00 Biocatalyzed Hydrolysis of Residual Oils and Proteins from Flax and Camelina Oilseed Press Cakes Using Lipase and Protease Oreste Piccolo Elisabetta Parodi Antonella Petri Piccolo, Oreste Parodi, Elisabetta Petri, Antonella 2022 ELETTRONICO https://hdl.handle.net/11568/1166078 https://doi.org/10.21926/cr.2204041 eng eng volume:2 issue:4 numberofpages:9 journal:CATALYSIS RESEARCH https://hdl.handle.net/11568/1166078 doi:10.21926/cr.2204041 Oilseed press cake biomass valorization biocatalyzed hydrolysi lipase protease free fatty acid amino acids info:eu-repo/semantics/article 2022 ftunivpisairis https://doi.org/10.21926/cr.2204041 2024-06-03T23:51:29Z In this study, a PoC (Proof of Concept) of a possible biomass valorization of flax and camelina oilseed press cakes was presented. Biocatalyzed hydrolysis of residual oils and proteins extracted from these wastes was studied. The biotransformation of oils was performed using commercial immobilized lipases, including Amano PS, Amano AK, and Candida Antarctica Lipase B (CALB). Acylglycerols were partially or fully hydrolyzed using Amano PS and AK. Triglycerides were not hydrolyzed by CALB, which behaved differently. Enzymatic hydrolysis of the proteins extracted from these cakes was performed using commercial proteases, including Amano Protease P and Amano Protease M. This was the first study to quantify the amino acids in the reaction products. The results were also compared to the hydrolysates obtained using 6 M HCl. Some differences were observed in the amino acid profiles depending on the enzyme used and the protein sample. Article in Journal/Newspaper Antarc* Antarctica ARPI - Archivio della Ricerca dell'Università di Pisa Catalysis Research 02 04 1 9 |
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Open Polar |
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ARPI - Archivio della Ricerca dell'Università di Pisa |
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ftunivpisairis |
language |
English |
topic |
Oilseed press cake biomass valorization biocatalyzed hydrolysi lipase protease free fatty acid amino acids |
spellingShingle |
Oilseed press cake biomass valorization biocatalyzed hydrolysi lipase protease free fatty acid amino acids Oreste Piccolo Elisabetta Parodi Antonella Petri Biocatalyzed Hydrolysis of Residual Oils and Proteins from Flax and Camelina Oilseed Press Cakes Using Lipase and Protease |
topic_facet |
Oilseed press cake biomass valorization biocatalyzed hydrolysi lipase protease free fatty acid amino acids |
description |
In this study, a PoC (Proof of Concept) of a possible biomass valorization of flax and camelina oilseed press cakes was presented. Biocatalyzed hydrolysis of residual oils and proteins extracted from these wastes was studied. The biotransformation of oils was performed using commercial immobilized lipases, including Amano PS, Amano AK, and Candida Antarctica Lipase B (CALB). Acylglycerols were partially or fully hydrolyzed using Amano PS and AK. Triglycerides were not hydrolyzed by CALB, which behaved differently. Enzymatic hydrolysis of the proteins extracted from these cakes was performed using commercial proteases, including Amano Protease P and Amano Protease M. This was the first study to quantify the amino acids in the reaction products. The results were also compared to the hydrolysates obtained using 6 M HCl. Some differences were observed in the amino acid profiles depending on the enzyme used and the protein sample. |
author2 |
Piccolo, Oreste Parodi, Elisabetta Petri, Antonella |
format |
Article in Journal/Newspaper |
author |
Oreste Piccolo Elisabetta Parodi Antonella Petri |
author_facet |
Oreste Piccolo Elisabetta Parodi Antonella Petri |
author_sort |
Oreste Piccolo |
title |
Biocatalyzed Hydrolysis of Residual Oils and Proteins from Flax and Camelina Oilseed Press Cakes Using Lipase and Protease |
title_short |
Biocatalyzed Hydrolysis of Residual Oils and Proteins from Flax and Camelina Oilseed Press Cakes Using Lipase and Protease |
title_full |
Biocatalyzed Hydrolysis of Residual Oils and Proteins from Flax and Camelina Oilseed Press Cakes Using Lipase and Protease |
title_fullStr |
Biocatalyzed Hydrolysis of Residual Oils and Proteins from Flax and Camelina Oilseed Press Cakes Using Lipase and Protease |
title_full_unstemmed |
Biocatalyzed Hydrolysis of Residual Oils and Proteins from Flax and Camelina Oilseed Press Cakes Using Lipase and Protease |
title_sort |
biocatalyzed hydrolysis of residual oils and proteins from flax and camelina oilseed press cakes using lipase and protease |
publishDate |
2022 |
url |
https://hdl.handle.net/11568/1166078 https://doi.org/10.21926/cr.2204041 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
volume:2 issue:4 numberofpages:9 journal:CATALYSIS RESEARCH https://hdl.handle.net/11568/1166078 doi:10.21926/cr.2204041 |
op_doi |
https://doi.org/10.21926/cr.2204041 |
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Catalysis Research |
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02 |
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04 |
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1 |
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9 |
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1802637965647675392 |