Molecular Characterization of Clistobothrium sp. Viable Plerocercoids in Fresh Longfin Inshore Squid (Doryteuthis pealeii) and Implications for Cephalopod Inspection
Cephalopods, an appreciated seafood product, are common hosts of marine cestodes. The aim of this work is to report visible alive plerocercoids in longfin inshore squid (Doryteuthis pealeii), a cephalopod species commercialized as fresh and whole in Italy. Seventy D. pealeii from the Northwest Atlan...
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Online Access: | http://hdl.handle.net/11568/1050048 https://doi.org/10.3390/pathogens9070596 https://www.mdpi.com/2076-0817/9/7/596 |
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ftunivpisairis:oai:arpi.unipi.it:11568/1050048 2024-04-14T08:16:45+00:00 Molecular Characterization of Clistobothrium sp. Viable Plerocercoids in Fresh Longfin Inshore Squid (Doryteuthis pealeii) and Implications for Cephalopod Inspection Lisa Guardone Alice Giusti Ewa Bilska-Zajac Renato Malandra Miroslaw Ró ̇zycki Andrea Armani Guardone, Lisa Giusti, Alice Bilska-Zajac, Ewa Malandra, Renato Ró ̇zycki, Miroslaw Armani, Andrea 2020 ELETTRONICO http://hdl.handle.net/11568/1050048 https://doi.org/10.3390/pathogens9070596 https://www.mdpi.com/2076-0817/9/7/596 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000557238100001 volume:9 issue:7 numberofpages:17 journal:PATHOGENS http://hdl.handle.net/11568/1050048 doi:10.3390/pathogens9070596 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85088242074 https://www.mdpi.com/2076-0817/9/7/596 info:eu-repo/semantics/openAccess cestode helminth cephalopod food hygiene defect visual inspection info:eu-repo/semantics/article 2020 ftunivpisairis https://doi.org/10.3390/pathogens9070596 2024-03-21T19:14:41Z Cephalopods, an appreciated seafood product, are common hosts of marine cestodes. The aim of this work is to report visible alive plerocercoids in longfin inshore squid (Doryteuthis pealeii), a cephalopod species commercialized as fresh and whole in Italy. Seventy D. pealeii from the Northwest Atlantic (FAO area 21) were collected and visually inspected. In total, 18 plerocercoid larvae were found in the viscera of 10 host specimens (P: 14.3% 95% CI 7.1–24.7; MI: 1.8, MA: 0.26; range 1–4) and molecularly analyzed targeting the variable D2 region of the large subunit (LSU) rRNA gene and the cytochrome c oxidase subunit I (COI) gene. The molecular characterization allowed to identify all the plerocercoids as Clistobothrium sp., a cestode of the Phyllobothriidae family with Lamnidae sharks as definitive hosts, and cephalopods as second intermediate hosts. These findings represent the first molecular record of Clistobothrium sp. in D. pealeii, thus contributing to elucidate its poorly known life cycle. Even if not affecting consumer’s health, these visible parasites may represent a reason for disgust for consumers. Therefore, the results suggest that Food Business Operators should also check for the presence of these visible parasites during inspection and underline the importance of a correct consumers’ education Article in Journal/Newspaper Northwest Atlantic ARPI - Archivio della Ricerca dell'Università di Pisa Pathogens 9 7 596 |
institution |
Open Polar |
collection |
ARPI - Archivio della Ricerca dell'Università di Pisa |
op_collection_id |
ftunivpisairis |
language |
English |
topic |
cestode helminth cephalopod food hygiene defect visual inspection |
spellingShingle |
cestode helminth cephalopod food hygiene defect visual inspection Lisa Guardone Alice Giusti Ewa Bilska-Zajac Renato Malandra Miroslaw Ró ̇zycki Andrea Armani Molecular Characterization of Clistobothrium sp. Viable Plerocercoids in Fresh Longfin Inshore Squid (Doryteuthis pealeii) and Implications for Cephalopod Inspection |
topic_facet |
cestode helminth cephalopod food hygiene defect visual inspection |
description |
Cephalopods, an appreciated seafood product, are common hosts of marine cestodes. The aim of this work is to report visible alive plerocercoids in longfin inshore squid (Doryteuthis pealeii), a cephalopod species commercialized as fresh and whole in Italy. Seventy D. pealeii from the Northwest Atlantic (FAO area 21) were collected and visually inspected. In total, 18 plerocercoid larvae were found in the viscera of 10 host specimens (P: 14.3% 95% CI 7.1–24.7; MI: 1.8, MA: 0.26; range 1–4) and molecularly analyzed targeting the variable D2 region of the large subunit (LSU) rRNA gene and the cytochrome c oxidase subunit I (COI) gene. The molecular characterization allowed to identify all the plerocercoids as Clistobothrium sp., a cestode of the Phyllobothriidae family with Lamnidae sharks as definitive hosts, and cephalopods as second intermediate hosts. These findings represent the first molecular record of Clistobothrium sp. in D. pealeii, thus contributing to elucidate its poorly known life cycle. Even if not affecting consumer’s health, these visible parasites may represent a reason for disgust for consumers. Therefore, the results suggest that Food Business Operators should also check for the presence of these visible parasites during inspection and underline the importance of a correct consumers’ education |
author2 |
Guardone, Lisa Giusti, Alice Bilska-Zajac, Ewa Malandra, Renato Ró ̇zycki, Miroslaw Armani, Andrea |
format |
Article in Journal/Newspaper |
author |
Lisa Guardone Alice Giusti Ewa Bilska-Zajac Renato Malandra Miroslaw Ró ̇zycki Andrea Armani |
author_facet |
Lisa Guardone Alice Giusti Ewa Bilska-Zajac Renato Malandra Miroslaw Ró ̇zycki Andrea Armani |
author_sort |
Lisa Guardone |
title |
Molecular Characterization of Clistobothrium sp. Viable Plerocercoids in Fresh Longfin Inshore Squid (Doryteuthis pealeii) and Implications for Cephalopod Inspection |
title_short |
Molecular Characterization of Clistobothrium sp. Viable Plerocercoids in Fresh Longfin Inshore Squid (Doryteuthis pealeii) and Implications for Cephalopod Inspection |
title_full |
Molecular Characterization of Clistobothrium sp. Viable Plerocercoids in Fresh Longfin Inshore Squid (Doryteuthis pealeii) and Implications for Cephalopod Inspection |
title_fullStr |
Molecular Characterization of Clistobothrium sp. Viable Plerocercoids in Fresh Longfin Inshore Squid (Doryteuthis pealeii) and Implications for Cephalopod Inspection |
title_full_unstemmed |
Molecular Characterization of Clistobothrium sp. Viable Plerocercoids in Fresh Longfin Inshore Squid (Doryteuthis pealeii) and Implications for Cephalopod Inspection |
title_sort |
molecular characterization of clistobothrium sp. viable plerocercoids in fresh longfin inshore squid (doryteuthis pealeii) and implications for cephalopod inspection |
publishDate |
2020 |
url |
http://hdl.handle.net/11568/1050048 https://doi.org/10.3390/pathogens9070596 https://www.mdpi.com/2076-0817/9/7/596 |
genre |
Northwest Atlantic |
genre_facet |
Northwest Atlantic |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000557238100001 volume:9 issue:7 numberofpages:17 journal:PATHOGENS http://hdl.handle.net/11568/1050048 doi:10.3390/pathogens9070596 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85088242074 https://www.mdpi.com/2076-0817/9/7/596 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/pathogens9070596 |
container_title |
Pathogens |
container_volume |
9 |
container_issue |
7 |
container_start_page |
596 |
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1796315495854505984 |