Molecular identification and prevalence of Dictyocaulus spp. (Trichostrongyloidea: Dictyocaulidae) in Swedish semi-domestic and free-living cervids
Lungs of 102 roe deer (Capreolus capreolus), 136 mouse (Alces alces), 68 fallow deer (Dama dama), and six red deer (Cervus elaphus) were examined during hunting seasons from 16 September 1997 to 1 March 2000. The aim was to determine the species composition and prevalence of Dictyocaulus lungworms i...
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ftunivphilosban:oai:www.ukdr.uplb.edu.ph:journal-articles-4267 2023-05-15T13:13:48+02:00 Molecular identification and prevalence of Dictyocaulus spp. (Trichostrongyloidea: Dictyocaulidae) in Swedish semi-domestic and free-living cervids Divina, Billy P. Wilhelmsson, Elisabeth Mörner, Torsten Mattsson, Jens G. Höglund, Johan 2021-04-21T15:07:26Z https://www.ukdr.uplb.edu.ph/journal-articles/3224 unknown University Knowledge Digital Repository https://www.ukdr.uplb.edu.ph/journal-articles/3224 Journal Article Cervids Dictyocaulus spp ELISA ITS2 Lungworms Molecular identification Sweden text 2021 ftunivphilosban 2023-03-05T06:54:15Z Lungs of 102 roe deer (Capreolus capreolus), 136 mouse (Alces alces), 68 fallow deer (Dama dama), and six red deer (Cervus elaphus) were examined during hunting seasons from 16 September 1997 to 1 March 2000. The aim was to determine the species composition and prevalence of Dictyocaulus lungworms in these hosts in Sweden. Worms were identified following polymerase chain reaction (PCR) amplification of the internal transcribed spacer of ribosomal DNA (ITS2), followed by hybridization with four species-specific oligonucleotides. In addition, 50 lungworms from five reindeer (Rangifer tarandus) from Norway were similarly analyzed A total of 399 worms were recovered and analyzed representing a range of 29-128 worms per host species. All specimens from roe deer were identified as Dictyocaulus capreolus, whereas those from red deer and reindeer were identical with D. eckerti. From moose, 73 (81.1%) of the worms were identified as D. capreolus whereas 17 (18.9%) were D. eckerti. The ITS2 sequence of fallow deer lungworms differed significantly when compared with the ITS2 of D. viviparus, D. capreolus, and D. eckerti. This indicated that fallow deer in Sweden may be infected with a new genotype of Dictyocaulus spp. Consequently, a specific probe designed for the ITS2 from this Dictyocaulus sp. hybridized exclusively with samples from lungworms of fallow deer. Interestingly, no D. viviparus were found in any of these hosts. The prevalence of infection in each host was as follows: D. capreolus in roe deer (14.7%) and moose (10.6%); D. eckerti in moose (0.7%) and red deer (33.3%); and Dictyocaulus sp. in fallow deer (10.3%). Regardless of lungworm species, the overall prevalence of Dictyocaulus spp. in these hosts was 12.2%. Prevalence between male and female animals and among the different age groups did not differ significantly. Finally an enzyme linked immunosorbent assay (ELISA) specific for patent D. viviparus infection in cattle was utilized to analyze hing tissue fluids from infected animals. All samples from roe ... Text Alces alces Rangifer tarandus University Knowledge Digital Repository UPLB (University of the Philippines Los Baños) Norway |
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Open Polar |
collection |
University Knowledge Digital Repository UPLB (University of the Philippines Los Baños) |
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ftunivphilosban |
language |
unknown |
topic |
Cervids Dictyocaulus spp ELISA ITS2 Lungworms Molecular identification Sweden |
spellingShingle |
Cervids Dictyocaulus spp ELISA ITS2 Lungworms Molecular identification Sweden Divina, Billy P. Wilhelmsson, Elisabeth Mörner, Torsten Mattsson, Jens G. Höglund, Johan Molecular identification and prevalence of Dictyocaulus spp. (Trichostrongyloidea: Dictyocaulidae) in Swedish semi-domestic and free-living cervids |
topic_facet |
Cervids Dictyocaulus spp ELISA ITS2 Lungworms Molecular identification Sweden |
description |
Lungs of 102 roe deer (Capreolus capreolus), 136 mouse (Alces alces), 68 fallow deer (Dama dama), and six red deer (Cervus elaphus) were examined during hunting seasons from 16 September 1997 to 1 March 2000. The aim was to determine the species composition and prevalence of Dictyocaulus lungworms in these hosts in Sweden. Worms were identified following polymerase chain reaction (PCR) amplification of the internal transcribed spacer of ribosomal DNA (ITS2), followed by hybridization with four species-specific oligonucleotides. In addition, 50 lungworms from five reindeer (Rangifer tarandus) from Norway were similarly analyzed A total of 399 worms were recovered and analyzed representing a range of 29-128 worms per host species. All specimens from roe deer were identified as Dictyocaulus capreolus, whereas those from red deer and reindeer were identical with D. eckerti. From moose, 73 (81.1%) of the worms were identified as D. capreolus whereas 17 (18.9%) were D. eckerti. The ITS2 sequence of fallow deer lungworms differed significantly when compared with the ITS2 of D. viviparus, D. capreolus, and D. eckerti. This indicated that fallow deer in Sweden may be infected with a new genotype of Dictyocaulus spp. Consequently, a specific probe designed for the ITS2 from this Dictyocaulus sp. hybridized exclusively with samples from lungworms of fallow deer. Interestingly, no D. viviparus were found in any of these hosts. The prevalence of infection in each host was as follows: D. capreolus in roe deer (14.7%) and moose (10.6%); D. eckerti in moose (0.7%) and red deer (33.3%); and Dictyocaulus sp. in fallow deer (10.3%). Regardless of lungworm species, the overall prevalence of Dictyocaulus spp. in these hosts was 12.2%. Prevalence between male and female animals and among the different age groups did not differ significantly. Finally an enzyme linked immunosorbent assay (ELISA) specific for patent D. viviparus infection in cattle was utilized to analyze hing tissue fluids from infected animals. All samples from roe ... |
format |
Text |
author |
Divina, Billy P. Wilhelmsson, Elisabeth Mörner, Torsten Mattsson, Jens G. Höglund, Johan |
author_facet |
Divina, Billy P. Wilhelmsson, Elisabeth Mörner, Torsten Mattsson, Jens G. Höglund, Johan |
author_sort |
Divina, Billy P. |
title |
Molecular identification and prevalence of Dictyocaulus spp. (Trichostrongyloidea: Dictyocaulidae) in Swedish semi-domestic and free-living cervids |
title_short |
Molecular identification and prevalence of Dictyocaulus spp. (Trichostrongyloidea: Dictyocaulidae) in Swedish semi-domestic and free-living cervids |
title_full |
Molecular identification and prevalence of Dictyocaulus spp. (Trichostrongyloidea: Dictyocaulidae) in Swedish semi-domestic and free-living cervids |
title_fullStr |
Molecular identification and prevalence of Dictyocaulus spp. (Trichostrongyloidea: Dictyocaulidae) in Swedish semi-domestic and free-living cervids |
title_full_unstemmed |
Molecular identification and prevalence of Dictyocaulus spp. (Trichostrongyloidea: Dictyocaulidae) in Swedish semi-domestic and free-living cervids |
title_sort |
molecular identification and prevalence of dictyocaulus spp. (trichostrongyloidea: dictyocaulidae) in swedish semi-domestic and free-living cervids |
publisher |
University Knowledge Digital Repository |
publishDate |
2021 |
url |
https://www.ukdr.uplb.edu.ph/journal-articles/3224 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Alces alces Rangifer tarandus |
genre_facet |
Alces alces Rangifer tarandus |
op_source |
Journal Article |
op_relation |
https://www.ukdr.uplb.edu.ph/journal-articles/3224 |
_version_ |
1766260505404506112 |