Transcriptomic responses of Antarctic clam Laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers
International audience In recent years micro- and nanoplastics and metal-oxide nanomaterials have been found in several environmental compartments. The Antarctic soft clam Laternula elliptica is an endemic Antarctic species having a wide distribution in the Southern Ocean. Being a filter-feeder, it...
Main Authors: | , , , , , , , , , , , , |
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Other Authors: | , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2024
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Online Access: | https://hal.science/hal-04615510 https://hal.science/hal-04615510/document https://hal.science/hal-04615510/file/Rondon-2024-EcotoxEnvironSaf-Transcriptomic-MANUSCRIT.pdf https://doi.org/10.1016/j.ecoenv.2024.116523 |
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Open Polar |
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Université de Perpignan: HAL |
op_collection_id |
ftunivperpignan |
language |
English |
topic |
Nanoplastic nanomaterials toxicity gene expression biomarkers Antarctic benthos [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology |
spellingShingle |
Nanoplastic nanomaterials toxicity gene expression biomarkers Antarctic benthos [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology Rondon, Rodolfo Valdés, Catalina Cosseau, Celine Bergami, Elisa Cárdenas, César Antonio Balbi, Teresa Pérez-Toledo, Carolina Garrido, Ignacio Perrois, Garance Chaparro, Cristian Corre, Erwan Corsi, Ilaria González-Aravena, Marcelo Transcriptomic responses of Antarctic clam Laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers |
topic_facet |
Nanoplastic nanomaterials toxicity gene expression biomarkers Antarctic benthos [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology |
description |
International audience In recent years micro- and nanoplastics and metal-oxide nanomaterials have been found in several environmental compartments. The Antarctic soft clam Laternula elliptica is an endemic Antarctic species having a wide distribution in the Southern Ocean. Being a filter-feeder, it could act as suitable bioindicator of pollution from nanoparticles also considering its sensitivity to various sources of stress. The present study aims to assess the impact of polystyrene nanoparticles (PS-NP) and the nanometal titanium-dioxide (n-TiO2) on genome-wide transcript expression of L. elliptica either alone and in combination and at two toxicological relevant concentrations (5 and 50 µg/L) during 96 h exposure. Transcript-target qRT-PCR was performed with the aim to identify suitable biomarkers of exposure and effects. As expected, at the highest concentration tested, the clustering was clearer between control and exposed clams. A total of 221 genes resulted differentially expressed in exposed clams and control ones, and 21 of them had functional annotation such as ribosomal proteins, antioxidant, ion transport (osmoregulation), acid-base balance, immunity, lipid metabolism, cell adhesion, cytoskeleton, apoptosis, chromatin condensation and cell signaling. At functional level, relevant transcripts were shared among some treatments and could be considered as general stress due to nanoparticle exposure. After applying transcript-target approach duplicating the number of clam samples, four ecologically relevant transcripts were revealed as biomarkers for PS-NP, n-TiO2 and their combination at 50 µg/L, that could be used for monitoring clams’ health status in different Antarctic localities. |
author2 |
The University of Texas Medical School at Houston Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) Università degli Studi di Modena e Reggio Emilia = University of Modena and Reggio Emilia (UNIMORE) Dipartimento di Scienze della Terra dell'Ambiente e della Vita (DISTAV) Università degli studi di Genova = University of Genoa (UniGe) Universidad Austral de Chile Biologie et Ecologie des Ecosystèmes Marins Profonds (BEEP) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS) ABiMS - Informatique et bioinformatique = Analysis and Bioinformatics for Marine Science (ABIMS) Fédération de recherche de Roscoff (FR2424) Station biologique de Roscoff = Roscoff Marine Station (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff = Roscoff Marine Station (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) Department of Environmental Sciences “G. Sarfatti” Università degli Studi di Siena = University of Siena (UNISI) |
format |
Article in Journal/Newspaper |
author |
Rondon, Rodolfo Valdés, Catalina Cosseau, Celine Bergami, Elisa Cárdenas, César Antonio Balbi, Teresa Pérez-Toledo, Carolina Garrido, Ignacio Perrois, Garance Chaparro, Cristian Corre, Erwan Corsi, Ilaria González-Aravena, Marcelo |
author_facet |
Rondon, Rodolfo Valdés, Catalina Cosseau, Celine Bergami, Elisa Cárdenas, César Antonio Balbi, Teresa Pérez-Toledo, Carolina Garrido, Ignacio Perrois, Garance Chaparro, Cristian Corre, Erwan Corsi, Ilaria González-Aravena, Marcelo |
author_sort |
Rondon, Rodolfo |
title |
Transcriptomic responses of Antarctic clam Laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers |
title_short |
Transcriptomic responses of Antarctic clam Laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers |
title_full |
Transcriptomic responses of Antarctic clam Laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers |
title_fullStr |
Transcriptomic responses of Antarctic clam Laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers |
title_full_unstemmed |
Transcriptomic responses of Antarctic clam Laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers |
title_sort |
transcriptomic responses of antarctic clam laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers |
publisher |
HAL CCSD |
publishDate |
2024 |
url |
https://hal.science/hal-04615510 https://hal.science/hal-04615510/document https://hal.science/hal-04615510/file/Rondon-2024-EcotoxEnvironSaf-Transcriptomic-MANUSCRIT.pdf https://doi.org/10.1016/j.ecoenv.2024.116523 |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Southern Ocean |
genre_facet |
Antarc* Antarctic Southern Ocean |
op_source |
ISSN: 0147-6513 EISSN: 1090-2414 Ecotoxicology and Environmental Safety https://hal.science/hal-04615510 Ecotoxicology and Environmental Safety, 2024, 280, pp.116523. ⟨10.1016/j.ecoenv.2024.116523⟩ https://www.sciencedirect.com/science/article/pii/S0147651324005992?via%3Dihub |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2024.116523 hal-04615510 https://hal.science/hal-04615510 https://hal.science/hal-04615510/document https://hal.science/hal-04615510/file/Rondon-2024-EcotoxEnvironSaf-Transcriptomic-MANUSCRIT.pdf doi:10.1016/j.ecoenv.2024.116523 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.ecoenv.2024.116523 |
_version_ |
1809822580083785728 |
spelling |
ftunivperpignan:oai:HAL:hal-04615510v1 2024-09-09T19:08:21+00:00 Transcriptomic responses of Antarctic clam Laternula elliptica to nanoparticles, at single and combined exposures reveal ecologically relevant biomarkers Rondon, Rodolfo Valdés, Catalina Cosseau, Celine Bergami, Elisa Cárdenas, César Antonio Balbi, Teresa Pérez-Toledo, Carolina Garrido, Ignacio Perrois, Garance Chaparro, Cristian Corre, Erwan Corsi, Ilaria González-Aravena, Marcelo The University of Texas Medical School at Houston Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) Università degli Studi di Modena e Reggio Emilia = University of Modena and Reggio Emilia (UNIMORE) Dipartimento di Scienze della Terra dell'Ambiente e della Vita (DISTAV) Università degli studi di Genova = University of Genoa (UniGe) Universidad Austral de Chile Biologie et Ecologie des Ecosystèmes Marins Profonds (BEEP) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS) ABiMS - Informatique et bioinformatique = Analysis and Bioinformatics for Marine Science (ABIMS) Fédération de recherche de Roscoff (FR2424) Station biologique de Roscoff = Roscoff Marine Station (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff = Roscoff Marine Station (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) Department of Environmental Sciences “G. Sarfatti” Università degli Studi di Siena = University of Siena (UNISI) 2024-07 https://hal.science/hal-04615510 https://hal.science/hal-04615510/document https://hal.science/hal-04615510/file/Rondon-2024-EcotoxEnvironSaf-Transcriptomic-MANUSCRIT.pdf https://doi.org/10.1016/j.ecoenv.2024.116523 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2024.116523 hal-04615510 https://hal.science/hal-04615510 https://hal.science/hal-04615510/document https://hal.science/hal-04615510/file/Rondon-2024-EcotoxEnvironSaf-Transcriptomic-MANUSCRIT.pdf doi:10.1016/j.ecoenv.2024.116523 info:eu-repo/semantics/OpenAccess ISSN: 0147-6513 EISSN: 1090-2414 Ecotoxicology and Environmental Safety https://hal.science/hal-04615510 Ecotoxicology and Environmental Safety, 2024, 280, pp.116523. ⟨10.1016/j.ecoenv.2024.116523⟩ https://www.sciencedirect.com/science/article/pii/S0147651324005992?via%3Dihub Nanoplastic nanomaterials toxicity gene expression biomarkers Antarctic benthos [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology info:eu-repo/semantics/article Journal articles 2024 ftunivperpignan https://doi.org/10.1016/j.ecoenv.2024.116523 2024-08-06T23:35:16Z International audience In recent years micro- and nanoplastics and metal-oxide nanomaterials have been found in several environmental compartments. The Antarctic soft clam Laternula elliptica is an endemic Antarctic species having a wide distribution in the Southern Ocean. Being a filter-feeder, it could act as suitable bioindicator of pollution from nanoparticles also considering its sensitivity to various sources of stress. The present study aims to assess the impact of polystyrene nanoparticles (PS-NP) and the nanometal titanium-dioxide (n-TiO2) on genome-wide transcript expression of L. elliptica either alone and in combination and at two toxicological relevant concentrations (5 and 50 µg/L) during 96 h exposure. Transcript-target qRT-PCR was performed with the aim to identify suitable biomarkers of exposure and effects. As expected, at the highest concentration tested, the clustering was clearer between control and exposed clams. A total of 221 genes resulted differentially expressed in exposed clams and control ones, and 21 of them had functional annotation such as ribosomal proteins, antioxidant, ion transport (osmoregulation), acid-base balance, immunity, lipid metabolism, cell adhesion, cytoskeleton, apoptosis, chromatin condensation and cell signaling. At functional level, relevant transcripts were shared among some treatments and could be considered as general stress due to nanoparticle exposure. After applying transcript-target approach duplicating the number of clam samples, four ecologically relevant transcripts were revealed as biomarkers for PS-NP, n-TiO2 and their combination at 50 µg/L, that could be used for monitoring clams’ health status in different Antarctic localities. Article in Journal/Newspaper Antarc* Antarctic Southern Ocean Université de Perpignan: HAL Antarctic Southern Ocean The Antarctic |