Reproductive cycle and temperature-related differences in baseline levels of HSP70 and metallothioneins in wild oyster populations of Crassostrea gigas
International audience Heat Shock Protein 70 (HSP70) are generalist stress proteins expressed in response to numerous environmental stresses. However, the baselines of these constitutive proteins are still unknown in many organisms and in particular marine intertidal species such as the Pacific oyst...
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ftunivperpignan:oai:HAL:hal-01025439v1 2024-02-11T10:03:02+01:00 Reproductive cycle and temperature-related differences in baseline levels of HSP70 and metallothioneins in wild oyster populations of Crassostrea gigas Meistertzheim, Anne-Leila Centre de formation et de recherche en environnement marin (CEFREM) Université de Perpignan Via Domitia (UPVD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2013-03-11 https://hal.univ-brest.fr/hal-01025439 en eng HAL CCSD Nova Science Publishers, Inc. ISBN: 978-162417571-8 hal-01025439 https://hal.univ-brest.fr/hal-01025439 Heat Shock Proteins: Classification, Functions and Applications https://hal.univ-brest.fr/hal-01025439 Heat Shock Proteins: Classification, Functions and Applications, Nova Science Publishers, Inc., pp.135-153, 2013, 978-162417571-8 Crassostrea gigas ELISA Gonad Heat Shock Proteins Maturation stage Metallothioneins Repartition area Reproduction Sex Temperature Thermal limit [SDV]Life Sciences [q-bio] info:eu-repo/semantics/bookPart Book sections 2013 ftunivperpignan 2024-01-23T23:45:02Z International audience Heat Shock Protein 70 (HSP70) are generalist stress proteins expressed in response to numerous environmental stresses. However, the baselines of these constitutive proteins are still unknown in many organisms and in particular marine intertidal species such as the Pacific oyster, Crassostrea gigas. I demonstrate that levels of heat shock proteins (HSP) and other stress proteins (metallothioneins, MTs) quantified by ELISA, remained similar in gills, mantle and digestive gland between oysters inhabiting cold and hot sites. In contrast, endogenous HSPs and MTs levels in gonad changed significantly during gametogenesis. In female gonads, the constitutive form of HSP70 and the MTs increased from immature to mature stages (about more than 3-fold) and decreased after spawning. In male gonads, the same expression patterns were observed, whereas protein levels were lower and decreased once fully mature. I hypothesize that the high level of stress proteins in eggs may increase survival of oyster progeny Book Part Crassostrea gigas Pacific oyster Université de Perpignan: HAL Pacific |
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Université de Perpignan: HAL |
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ftunivperpignan |
language |
English |
topic |
Crassostrea gigas ELISA Gonad Heat Shock Proteins Maturation stage Metallothioneins Repartition area Reproduction Sex Temperature Thermal limit [SDV]Life Sciences [q-bio] |
spellingShingle |
Crassostrea gigas ELISA Gonad Heat Shock Proteins Maturation stage Metallothioneins Repartition area Reproduction Sex Temperature Thermal limit [SDV]Life Sciences [q-bio] Meistertzheim, Anne-Leila Reproductive cycle and temperature-related differences in baseline levels of HSP70 and metallothioneins in wild oyster populations of Crassostrea gigas |
topic_facet |
Crassostrea gigas ELISA Gonad Heat Shock Proteins Maturation stage Metallothioneins Repartition area Reproduction Sex Temperature Thermal limit [SDV]Life Sciences [q-bio] |
description |
International audience Heat Shock Protein 70 (HSP70) are generalist stress proteins expressed in response to numerous environmental stresses. However, the baselines of these constitutive proteins are still unknown in many organisms and in particular marine intertidal species such as the Pacific oyster, Crassostrea gigas. I demonstrate that levels of heat shock proteins (HSP) and other stress proteins (metallothioneins, MTs) quantified by ELISA, remained similar in gills, mantle and digestive gland between oysters inhabiting cold and hot sites. In contrast, endogenous HSPs and MTs levels in gonad changed significantly during gametogenesis. In female gonads, the constitutive form of HSP70 and the MTs increased from immature to mature stages (about more than 3-fold) and decreased after spawning. In male gonads, the same expression patterns were observed, whereas protein levels were lower and decreased once fully mature. I hypothesize that the high level of stress proteins in eggs may increase survival of oyster progeny |
author2 |
Centre de formation et de recherche en environnement marin (CEFREM) Université de Perpignan Via Domitia (UPVD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Book Part |
author |
Meistertzheim, Anne-Leila |
author_facet |
Meistertzheim, Anne-Leila |
author_sort |
Meistertzheim, Anne-Leila |
title |
Reproductive cycle and temperature-related differences in baseline levels of HSP70 and metallothioneins in wild oyster populations of Crassostrea gigas |
title_short |
Reproductive cycle and temperature-related differences in baseline levels of HSP70 and metallothioneins in wild oyster populations of Crassostrea gigas |
title_full |
Reproductive cycle and temperature-related differences in baseline levels of HSP70 and metallothioneins in wild oyster populations of Crassostrea gigas |
title_fullStr |
Reproductive cycle and temperature-related differences in baseline levels of HSP70 and metallothioneins in wild oyster populations of Crassostrea gigas |
title_full_unstemmed |
Reproductive cycle and temperature-related differences in baseline levels of HSP70 and metallothioneins in wild oyster populations of Crassostrea gigas |
title_sort |
reproductive cycle and temperature-related differences in baseline levels of hsp70 and metallothioneins in wild oyster populations of crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal.univ-brest.fr/hal-01025439 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Heat Shock Proteins: Classification, Functions and Applications https://hal.univ-brest.fr/hal-01025439 Heat Shock Proteins: Classification, Functions and Applications, Nova Science Publishers, Inc., pp.135-153, 2013, 978-162417571-8 |
op_relation |
ISBN: 978-162417571-8 hal-01025439 https://hal.univ-brest.fr/hal-01025439 |
_version_ |
1790599156795965440 |