Antioxidant enzyme activity of filamentous fungi isolated from Livingston Island, Maritime Antarctica
From 18 soil samples taken in the vicinity of the permanent Bulgarian Antarctic base "St. Kliment Ohridski" (62° 38 '29 "S, 60° 21’ 53’’ W) on Livingston Island, 109 filamentous fungi were isolated on selective media. The most widespread fungal species were members of the genera...
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ftunivpavia:oai:iris.unipv.it:11571/210216 2024-02-27T08:34:27+00:00 Antioxidant enzyme activity of filamentous fungi isolated from Livingston Island, Maritime Antarctica TOSI, SOLVEIG Kostadinova N. Krumova E. Pashova S. Dishliiska V. Spassova B. Vassilev S. Angelova M. Tosi, Solveig Kostadinova, N. Krumova, E. Pashova, S. Dishliiska, V. Spassova, B. Vassilev, S. Angelova, M. 2010 STAMPA http://hdl.handle.net/11571/210216 https://doi.org/10.1007/s00300-010-0812-1 http://www.springerlink.com/content/eq662tg1182g33m2/ eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000280802000007 volume:33 firstpage:1227 lastpage:1237 numberofpages:11 journal:POLAR BIOLOGY http://hdl.handle.net/11571/210216 doi:10.1007/s00300-010-0812-1 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-77955553058 http://www.springerlink.com/content/eq662tg1182g33m2/ ANTARCTIC FUNGI LIVINGSTON ISLAND SUPEROXIDE DISMUTASE CATALASE COLD-ACTIVE ENZYME BIOREACTOR CULTURES info:eu-repo/semantics/article 2010 ftunivpavia https://doi.org/10.1007/s00300-010-0812-1 2024-01-31T17:47:10Z From 18 soil samples taken in the vicinity of the permanent Bulgarian Antarctic base "St. Kliment Ohridski" (62° 38 '29 "S, 60° 21’ 53’’ W) on Livingston Island, 109 filamentous fungi were isolated on selective media. The most widespread fungal species were members of the genera Cladosporium, Geomyces, Penicillium and Aspergillus. Other species, already recorded for Antarctic environments were also isolated: Lecanicillium muscarium, Epicoccum nigrum and Alternaria alternata. Thirty strains demonstrating good growth were screened for superoxide dismutase (SOD) and catalase (CAT) activities and 6 of them showed high enzyme activity. The tested strains produced SOD with higher activity at 15°C than at 30°C suggesting that this enzyme is cold-active. The best producer of cold-active SOD, A. glaucus 363 cultivated in bioreactors demonstrated optimal growth temperature at 25°C and maximum enzyme activities at 25°C and 30°C for SOD and CAT, respectively. The electrophoretical analysis showed that the fungus possesses Cu/Zn-SOD. Article in Journal/Newspaper Antarc* Antarctic Antarctica Livingston Island IRIS UNIPV (Università degli studi di Pavia) Antarctic Livingston Island ENVELOPE(-60.500,-60.500,-62.600,-62.600) Polar Biology 33 9 1227 1237 |
institution |
Open Polar |
collection |
IRIS UNIPV (Università degli studi di Pavia) |
op_collection_id |
ftunivpavia |
language |
English |
topic |
ANTARCTIC FUNGI LIVINGSTON ISLAND SUPEROXIDE DISMUTASE CATALASE COLD-ACTIVE ENZYME BIOREACTOR CULTURES |
spellingShingle |
ANTARCTIC FUNGI LIVINGSTON ISLAND SUPEROXIDE DISMUTASE CATALASE COLD-ACTIVE ENZYME BIOREACTOR CULTURES TOSI, SOLVEIG Kostadinova N. Krumova E. Pashova S. Dishliiska V. Spassova B. Vassilev S. Angelova M. Antioxidant enzyme activity of filamentous fungi isolated from Livingston Island, Maritime Antarctica |
topic_facet |
ANTARCTIC FUNGI LIVINGSTON ISLAND SUPEROXIDE DISMUTASE CATALASE COLD-ACTIVE ENZYME BIOREACTOR CULTURES |
description |
From 18 soil samples taken in the vicinity of the permanent Bulgarian Antarctic base "St. Kliment Ohridski" (62° 38 '29 "S, 60° 21’ 53’’ W) on Livingston Island, 109 filamentous fungi were isolated on selective media. The most widespread fungal species were members of the genera Cladosporium, Geomyces, Penicillium and Aspergillus. Other species, already recorded for Antarctic environments were also isolated: Lecanicillium muscarium, Epicoccum nigrum and Alternaria alternata. Thirty strains demonstrating good growth were screened for superoxide dismutase (SOD) and catalase (CAT) activities and 6 of them showed high enzyme activity. The tested strains produced SOD with higher activity at 15°C than at 30°C suggesting that this enzyme is cold-active. The best producer of cold-active SOD, A. glaucus 363 cultivated in bioreactors demonstrated optimal growth temperature at 25°C and maximum enzyme activities at 25°C and 30°C for SOD and CAT, respectively. The electrophoretical analysis showed that the fungus possesses Cu/Zn-SOD. |
author2 |
Tosi, Solveig Kostadinova, N. Krumova, E. Pashova, S. Dishliiska, V. Spassova, B. Vassilev, S. Angelova, M. |
format |
Article in Journal/Newspaper |
author |
TOSI, SOLVEIG Kostadinova N. Krumova E. Pashova S. Dishliiska V. Spassova B. Vassilev S. Angelova M. |
author_facet |
TOSI, SOLVEIG Kostadinova N. Krumova E. Pashova S. Dishliiska V. Spassova B. Vassilev S. Angelova M. |
author_sort |
TOSI, SOLVEIG |
title |
Antioxidant enzyme activity of filamentous fungi isolated from Livingston Island, Maritime Antarctica |
title_short |
Antioxidant enzyme activity of filamentous fungi isolated from Livingston Island, Maritime Antarctica |
title_full |
Antioxidant enzyme activity of filamentous fungi isolated from Livingston Island, Maritime Antarctica |
title_fullStr |
Antioxidant enzyme activity of filamentous fungi isolated from Livingston Island, Maritime Antarctica |
title_full_unstemmed |
Antioxidant enzyme activity of filamentous fungi isolated from Livingston Island, Maritime Antarctica |
title_sort |
antioxidant enzyme activity of filamentous fungi isolated from livingston island, maritime antarctica |
publishDate |
2010 |
url |
http://hdl.handle.net/11571/210216 https://doi.org/10.1007/s00300-010-0812-1 http://www.springerlink.com/content/eq662tg1182g33m2/ |
long_lat |
ENVELOPE(-60.500,-60.500,-62.600,-62.600) |
geographic |
Antarctic Livingston Island |
geographic_facet |
Antarctic Livingston Island |
genre |
Antarc* Antarctic Antarctica Livingston Island |
genre_facet |
Antarc* Antarctic Antarctica Livingston Island |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000280802000007 volume:33 firstpage:1227 lastpage:1237 numberofpages:11 journal:POLAR BIOLOGY http://hdl.handle.net/11571/210216 doi:10.1007/s00300-010-0812-1 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-77955553058 http://www.springerlink.com/content/eq662tg1182g33m2/ |
op_doi |
https://doi.org/10.1007/s00300-010-0812-1 |
container_title |
Polar Biology |
container_volume |
33 |
container_issue |
9 |
container_start_page |
1227 |
op_container_end_page |
1237 |
_version_ |
1792055797418557440 |