Antarctic Soil Metabolomics: A Pilot Study
In Antarctica, ice-free areas can be found along the coast, on mountain peaks, and in the McMurdo Dry Valleys, where microorganisms well-adapted to harsh conditions can survive and reproduce. Metabolic analyses can shed light on the survival mechanisms of Antarctic soil communities from both coastal...
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ftunivpavia:oai:iris.unipv.it:11571/1482916 2024-04-14T08:02:58+00:00 Antarctic Soil Metabolomics: A Pilot Study Ciaramelli C. Palmioli A. Brioschi M. Viglio S. D'Amato M. Iadarola P. Tosi S. Zucconi L. Airoldi C. Ciaramelli, C. Palmioli, A. Brioschi, M. Viglio, S. D'Amato, M. Iadarola, P. Tosi, S. Zucconi, L. Airoldi, C. 2023 ELETTRONICO https://hdl.handle.net/11571/1482916 https://doi.org/10.3390/ijms241512340 https://www.mdpi.com/1422-0067/24/15/12340 eng eng info:eu-repo/semantics/altIdentifier/pmid/37569716 info:eu-repo/semantics/altIdentifier/wos/WOS:001045575700001 volume:24 issue:15 firstpage:12340 lastpage:12359 numberofpages:20 journal:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES https://hdl.handle.net/11571/1482916 doi:10.3390/ijms241512340 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85167752246 https://www.mdpi.com/1422-0067/24/15/12340 Antarctica NMR spectroscopy mass spectrometry metabolites info:eu-repo/semantics/article 2023 ftunivpavia https://doi.org/10.3390/ijms241512340 2024-03-21T15:45:52Z In Antarctica, ice-free areas can be found along the coast, on mountain peaks, and in the McMurdo Dry Valleys, where microorganisms well-adapted to harsh conditions can survive and reproduce. Metabolic analyses can shed light on the survival mechanisms of Antarctic soil communities from both coastal sites, under different plant coverage stages, and inner sites where slow-growing or dormant microorganisms, low water availability, salt accumulation, and a limited number of primary producers make metabolomic profiling difficult. Here, we report, for the first time, an efficient protocol for the extraction and the metabolic profiling of Antarctic soils based on the combination of NMR spectroscopy and mass spectrometry (MS). This approach was set up on samples harvested along different localities of Victoria Land, in continental Antarctica, devoid of or covered by differently developed biological crusts. NMR allowed for the identification of thirty metabolites (mainly sugars, amino acids, and organic acids) and the quantification of just over twenty of them. UPLC-MS analysis identified more than twenty other metabolites, in particular flavonoids, medium- and long-chain fatty acids, benzoic acid derivatives, anthracenes, and quinones. Our results highlighted the complementarity of the two analytical techniques. Moreover, we demonstrated that their combined use represents the "gold standard" for the qualitative and quantitative analysis of little-explored samples, such as those collected from Antarctic soils. Article in Journal/Newspaper Antarc* Antarctic Antarctica McMurdo Dry Valleys Victoria Land IRIS UNIPV (Università degli studi di Pavia) Antarctic McMurdo Dry Valleys Victoria Land International Journal of Molecular Sciences 24 15 12340 |
institution |
Open Polar |
collection |
IRIS UNIPV (Università degli studi di Pavia) |
op_collection_id |
ftunivpavia |
language |
English |
topic |
Antarctica NMR spectroscopy mass spectrometry metabolites |
spellingShingle |
Antarctica NMR spectroscopy mass spectrometry metabolites Ciaramelli C. Palmioli A. Brioschi M. Viglio S. D'Amato M. Iadarola P. Tosi S. Zucconi L. Airoldi C. Antarctic Soil Metabolomics: A Pilot Study |
topic_facet |
Antarctica NMR spectroscopy mass spectrometry metabolites |
description |
In Antarctica, ice-free areas can be found along the coast, on mountain peaks, and in the McMurdo Dry Valleys, where microorganisms well-adapted to harsh conditions can survive and reproduce. Metabolic analyses can shed light on the survival mechanisms of Antarctic soil communities from both coastal sites, under different plant coverage stages, and inner sites where slow-growing or dormant microorganisms, low water availability, salt accumulation, and a limited number of primary producers make metabolomic profiling difficult. Here, we report, for the first time, an efficient protocol for the extraction and the metabolic profiling of Antarctic soils based on the combination of NMR spectroscopy and mass spectrometry (MS). This approach was set up on samples harvested along different localities of Victoria Land, in continental Antarctica, devoid of or covered by differently developed biological crusts. NMR allowed for the identification of thirty metabolites (mainly sugars, amino acids, and organic acids) and the quantification of just over twenty of them. UPLC-MS analysis identified more than twenty other metabolites, in particular flavonoids, medium- and long-chain fatty acids, benzoic acid derivatives, anthracenes, and quinones. Our results highlighted the complementarity of the two analytical techniques. Moreover, we demonstrated that their combined use represents the "gold standard" for the qualitative and quantitative analysis of little-explored samples, such as those collected from Antarctic soils. |
author2 |
Ciaramelli, C. Palmioli, A. Brioschi, M. Viglio, S. D'Amato, M. Iadarola, P. Tosi, S. Zucconi, L. Airoldi, C. |
format |
Article in Journal/Newspaper |
author |
Ciaramelli C. Palmioli A. Brioschi M. Viglio S. D'Amato M. Iadarola P. Tosi S. Zucconi L. Airoldi C. |
author_facet |
Ciaramelli C. Palmioli A. Brioschi M. Viglio S. D'Amato M. Iadarola P. Tosi S. Zucconi L. Airoldi C. |
author_sort |
Ciaramelli C. |
title |
Antarctic Soil Metabolomics: A Pilot Study |
title_short |
Antarctic Soil Metabolomics: A Pilot Study |
title_full |
Antarctic Soil Metabolomics: A Pilot Study |
title_fullStr |
Antarctic Soil Metabolomics: A Pilot Study |
title_full_unstemmed |
Antarctic Soil Metabolomics: A Pilot Study |
title_sort |
antarctic soil metabolomics: a pilot study |
publishDate |
2023 |
url |
https://hdl.handle.net/11571/1482916 https://doi.org/10.3390/ijms241512340 https://www.mdpi.com/1422-0067/24/15/12340 |
geographic |
Antarctic McMurdo Dry Valleys Victoria Land |
geographic_facet |
Antarctic McMurdo Dry Valleys Victoria Land |
genre |
Antarc* Antarctic Antarctica McMurdo Dry Valleys Victoria Land |
genre_facet |
Antarc* Antarctic Antarctica McMurdo Dry Valleys Victoria Land |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/37569716 info:eu-repo/semantics/altIdentifier/wos/WOS:001045575700001 volume:24 issue:15 firstpage:12340 lastpage:12359 numberofpages:20 journal:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES https://hdl.handle.net/11571/1482916 doi:10.3390/ijms241512340 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85167752246 https://www.mdpi.com/1422-0067/24/15/12340 |
op_doi |
https://doi.org/10.3390/ijms241512340 |
container_title |
International Journal of Molecular Sciences |
container_volume |
24 |
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15 |
container_start_page |
12340 |
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1796319040143097856 |