Metabolomics to Study the Impact of Diazepam and Irbesartan on Glass Eels (Anguilla Anguilla) and the Differences in their Migratory Phenotypes
International audience Since glass eels (Anguilla anguilla) are continuously exposed to contamination throughout their migratory journey through the estuaries, to a certain extent the fall in the population of this endangered species may be due to this exposure, which is especially acute in estuarie...
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2023
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ftunivpau:oai:HAL:hal-04238347v1 2024-05-12T07:52:58+00:00 Metabolomics to Study the Impact of Diazepam and Irbesartan on Glass Eels (Anguilla Anguilla) and the Differences in their Migratory Phenotypes Alvarez, Iker Bolliet, Valérie Lopez, Naroa Olivares, Maitane Monperrus, Mathilde Etxebarria, Nestor Universidad del Pais Vasco / Euskal Herriko Unibertsitatea Espagne (UPV/EHU) Ecologie Comportementale et Biologie des Populations de Poissons (ECOBIOP) Université de Pau et des Pays de l'Adour (UPPA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM) Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) Dublin, France 2023-04-30 https://univ-pau.hal.science/hal-04238347 en eng HAL CCSD hal-04238347 https://univ-pau.hal.science/hal-04238347 SETAC Europe 33. Annual meeting https://univ-pau.hal.science/hal-04238347 SETAC Europe 33. Annual meeting, Apr 2023, Dublin, France [CHIM]Chemical Sciences [SDE]Environmental Sciences info:eu-repo/semantics/conferenceObject Conference poster 2023 ftunivpau 2024-04-12T00:07:00Z International audience Since glass eels (Anguilla anguilla) are continuously exposed to contamination throughout their migratory journey through the estuaries, to a certain extent the fall in the population of this endangered species may be due to this exposure, which is especially acute in estuaries under high urban pressure. Metabolomics was used in this study to assess the effects of the previously selected contaminants, diazepam, and irbesartan, on glass eels. An exposure experiment to diazepam, irbesartan, and their mixture was carried out over 7 days followed by 7 days of depuration phase. After exposure, glass eels were individually killed using a lethal bath of anaesthesia, and then an unbiased sample extraction method was used to extract separately the polar metabolome and the lipidome. The polar metabolome was submitted to targeted and non-targeted analysis, whereas for the lipidome only the nontargeted analysis was carried out. A combined strategy using partial least squares discriminant analysis and statistical analysis (ANOVA, t-test, and fold-change analysis) was used to identify the metabolites altered in the exposed groups with respect to the control group. The results of the polar metabolome analysis revealed that glass eels exposed to diazepam-irbesartan mixture were the most impacted ones, with altered levels for 11 metabolites, some of them belonging to the energetic metabolism, which was confirmed to be sensitive to these contaminants. Additionally, seven lipids were also found ysregulated after exposure to the mixture. In the same vein, the same metabolomics approach was also used to find the differences in the metabolic profile of the two behavioural phenotypes of glass eels (migrants and nonmigrants). Nonmigrant glass eels showed lower overall metabolic values than migrant glass eels, and the lipidome analysis also showed alterations in the levels of eight cholesterol esters. These two findings offer new research perspectives to explain this facultative migration of glass eels and ... Conference Object Anguilla anguilla HAL e2s UPPA (Université de Pau et des Pays de l'Adour) |
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Open Polar |
collection |
HAL e2s UPPA (Université de Pau et des Pays de l'Adour) |
op_collection_id |
ftunivpau |
language |
English |
topic |
[CHIM]Chemical Sciences [SDE]Environmental Sciences |
spellingShingle |
[CHIM]Chemical Sciences [SDE]Environmental Sciences Alvarez, Iker Bolliet, Valérie Lopez, Naroa Olivares, Maitane Monperrus, Mathilde Etxebarria, Nestor Metabolomics to Study the Impact of Diazepam and Irbesartan on Glass Eels (Anguilla Anguilla) and the Differences in their Migratory Phenotypes |
topic_facet |
[CHIM]Chemical Sciences [SDE]Environmental Sciences |
description |
International audience Since glass eels (Anguilla anguilla) are continuously exposed to contamination throughout their migratory journey through the estuaries, to a certain extent the fall in the population of this endangered species may be due to this exposure, which is especially acute in estuaries under high urban pressure. Metabolomics was used in this study to assess the effects of the previously selected contaminants, diazepam, and irbesartan, on glass eels. An exposure experiment to diazepam, irbesartan, and their mixture was carried out over 7 days followed by 7 days of depuration phase. After exposure, glass eels were individually killed using a lethal bath of anaesthesia, and then an unbiased sample extraction method was used to extract separately the polar metabolome and the lipidome. The polar metabolome was submitted to targeted and non-targeted analysis, whereas for the lipidome only the nontargeted analysis was carried out. A combined strategy using partial least squares discriminant analysis and statistical analysis (ANOVA, t-test, and fold-change analysis) was used to identify the metabolites altered in the exposed groups with respect to the control group. The results of the polar metabolome analysis revealed that glass eels exposed to diazepam-irbesartan mixture were the most impacted ones, with altered levels for 11 metabolites, some of them belonging to the energetic metabolism, which was confirmed to be sensitive to these contaminants. Additionally, seven lipids were also found ysregulated after exposure to the mixture. In the same vein, the same metabolomics approach was also used to find the differences in the metabolic profile of the two behavioural phenotypes of glass eels (migrants and nonmigrants). Nonmigrant glass eels showed lower overall metabolic values than migrant glass eels, and the lipidome analysis also showed alterations in the levels of eight cholesterol esters. These two findings offer new research perspectives to explain this facultative migration of glass eels and ... |
author2 |
Universidad del Pais Vasco / Euskal Herriko Unibertsitatea Espagne (UPV/EHU) Ecologie Comportementale et Biologie des Populations de Poissons (ECOBIOP) Université de Pau et des Pays de l'Adour (UPPA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM) Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Conference Object |
author |
Alvarez, Iker Bolliet, Valérie Lopez, Naroa Olivares, Maitane Monperrus, Mathilde Etxebarria, Nestor |
author_facet |
Alvarez, Iker Bolliet, Valérie Lopez, Naroa Olivares, Maitane Monperrus, Mathilde Etxebarria, Nestor |
author_sort |
Alvarez, Iker |
title |
Metabolomics to Study the Impact of Diazepam and Irbesartan on Glass Eels (Anguilla Anguilla) and the Differences in their Migratory Phenotypes |
title_short |
Metabolomics to Study the Impact of Diazepam and Irbesartan on Glass Eels (Anguilla Anguilla) and the Differences in their Migratory Phenotypes |
title_full |
Metabolomics to Study the Impact of Diazepam and Irbesartan on Glass Eels (Anguilla Anguilla) and the Differences in their Migratory Phenotypes |
title_fullStr |
Metabolomics to Study the Impact of Diazepam and Irbesartan on Glass Eels (Anguilla Anguilla) and the Differences in their Migratory Phenotypes |
title_full_unstemmed |
Metabolomics to Study the Impact of Diazepam and Irbesartan on Glass Eels (Anguilla Anguilla) and the Differences in their Migratory Phenotypes |
title_sort |
metabolomics to study the impact of diazepam and irbesartan on glass eels (anguilla anguilla) and the differences in their migratory phenotypes |
publisher |
HAL CCSD |
publishDate |
2023 |
url |
https://univ-pau.hal.science/hal-04238347 |
op_coverage |
Dublin, France |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
SETAC Europe 33. Annual meeting https://univ-pau.hal.science/hal-04238347 SETAC Europe 33. Annual meeting, Apr 2023, Dublin, France |
op_relation |
hal-04238347 https://univ-pau.hal.science/hal-04238347 |
_version_ |
1798839271330676736 |