Multi-element speciation of metalloproteins in fish tissue using size-exclusion chromatography coupled "on-line" with ICP-isotope dilution-time-of-flight-mass spectrometry
International audience An on-line isotope dilution (ID) method in combination with the coupling of size-exclusion high-performance liquid chromatography (SE-HPLC) to an axial inductively coupled plasma time-of-flight mass spectrometer (ICP-TOFMS) was applied to the quantitative speciation of Cu, Zn...
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ftunivpau:oai:HAL:hal-00291360v1 2023-11-12T04:16:49+01:00 Multi-element speciation of metalloproteins in fish tissue using size-exclusion chromatography coupled "on-line" with ICP-isotope dilution-time-of-flight-mass spectrometry Goenaga Infante, H. van Campenhout, K. Schaumlöffel, Dirk Blust, R. Adams, F.C. Laboratoire de Chimie Analytique Bio-Inorganique et Environnement (LCABIE) Université de Pau et des Pays de l'Adour (UPPA)-Centre National de la Recherche Scientifique (CNRS) 2003 https://hal.science/hal-00291360 https://doi.org/10.1039/b212889f en eng HAL CCSD Royal Society of Chemistry info:eu-repo/semantics/altIdentifier/doi/10.1039/b212889f hal-00291360 https://hal.science/hal-00291360 doi:10.1039/b212889f ISSN: 0003-2654 EISSN: 1364-5528 Analyst https://hal.science/hal-00291360 Analyst, 2003, 128 (6), pp.651-657. ⟨10.1039/b212889f⟩ [CHIM.ANAL]Chemical Sciences/Analytical chemistry info:eu-repo/semantics/article Journal articles 2003 ftunivpau https://doi.org/10.1039/b212889f 2023-10-15T20:40:36Z International audience An on-line isotope dilution (ID) method in combination with the coupling of size-exclusion high-performance liquid chromatography (SE-HPLC) to an axial inductively coupled plasma time-of-flight mass spectrometer (ICP-TOFMS) was applied to the quantitative speciation of Cu, Zn and Cd in carp and eel cytosols. Quantitative information on the distribution of essential and toxic metals between the different molecular weight fractions in kidney cytosols of control and Cd exposed carp was obtained with species-unspecific spiking by post-column addition of the enriched isotopes 65Cu, 67Zn and 106Cd. The isotope ratios 63Cu/65Cu, 64Zn/67Zn and 114Cd/106Cd, were monitored by ICP-TOFMS in transient signal mode. The determination of the total cytosolic element concentration was performed by flow injection conventional nebulization ICP-TOFMS. Speciation differences were encountered between control and Cd-stressed carp, indicating that quantitative speciation studies might be useful for ecotoxicological and biomonitoring purposes. Application of this methodology to quantitation of metals associated with metallothioneins (MT) in European eel, sampled at three different sites in Flanders, Belgium was also addressed. A significant increase of the amount of Cd, Cu and Zn bound to hepatic MT was observed with the increase of the total metal and MT concentrations of hepatic eel tissue. Article in Journal/Newspaper European eel HAL e2s UPPA (Université de Pau et des Pays de l'Adour) The Analyst 128 6 651 657 |
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Open Polar |
collection |
HAL e2s UPPA (Université de Pau et des Pays de l'Adour) |
op_collection_id |
ftunivpau |
language |
English |
topic |
[CHIM.ANAL]Chemical Sciences/Analytical chemistry |
spellingShingle |
[CHIM.ANAL]Chemical Sciences/Analytical chemistry Goenaga Infante, H. van Campenhout, K. Schaumlöffel, Dirk Blust, R. Adams, F.C. Multi-element speciation of metalloproteins in fish tissue using size-exclusion chromatography coupled "on-line" with ICP-isotope dilution-time-of-flight-mass spectrometry |
topic_facet |
[CHIM.ANAL]Chemical Sciences/Analytical chemistry |
description |
International audience An on-line isotope dilution (ID) method in combination with the coupling of size-exclusion high-performance liquid chromatography (SE-HPLC) to an axial inductively coupled plasma time-of-flight mass spectrometer (ICP-TOFMS) was applied to the quantitative speciation of Cu, Zn and Cd in carp and eel cytosols. Quantitative information on the distribution of essential and toxic metals between the different molecular weight fractions in kidney cytosols of control and Cd exposed carp was obtained with species-unspecific spiking by post-column addition of the enriched isotopes 65Cu, 67Zn and 106Cd. The isotope ratios 63Cu/65Cu, 64Zn/67Zn and 114Cd/106Cd, were monitored by ICP-TOFMS in transient signal mode. The determination of the total cytosolic element concentration was performed by flow injection conventional nebulization ICP-TOFMS. Speciation differences were encountered between control and Cd-stressed carp, indicating that quantitative speciation studies might be useful for ecotoxicological and biomonitoring purposes. Application of this methodology to quantitation of metals associated with metallothioneins (MT) in European eel, sampled at three different sites in Flanders, Belgium was also addressed. A significant increase of the amount of Cd, Cu and Zn bound to hepatic MT was observed with the increase of the total metal and MT concentrations of hepatic eel tissue. |
author2 |
Laboratoire de Chimie Analytique Bio-Inorganique et Environnement (LCABIE) Université de Pau et des Pays de l'Adour (UPPA)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Goenaga Infante, H. van Campenhout, K. Schaumlöffel, Dirk Blust, R. Adams, F.C. |
author_facet |
Goenaga Infante, H. van Campenhout, K. Schaumlöffel, Dirk Blust, R. Adams, F.C. |
author_sort |
Goenaga Infante, H. |
title |
Multi-element speciation of metalloproteins in fish tissue using size-exclusion chromatography coupled "on-line" with ICP-isotope dilution-time-of-flight-mass spectrometry |
title_short |
Multi-element speciation of metalloproteins in fish tissue using size-exclusion chromatography coupled "on-line" with ICP-isotope dilution-time-of-flight-mass spectrometry |
title_full |
Multi-element speciation of metalloproteins in fish tissue using size-exclusion chromatography coupled "on-line" with ICP-isotope dilution-time-of-flight-mass spectrometry |
title_fullStr |
Multi-element speciation of metalloproteins in fish tissue using size-exclusion chromatography coupled "on-line" with ICP-isotope dilution-time-of-flight-mass spectrometry |
title_full_unstemmed |
Multi-element speciation of metalloproteins in fish tissue using size-exclusion chromatography coupled "on-line" with ICP-isotope dilution-time-of-flight-mass spectrometry |
title_sort |
multi-element speciation of metalloproteins in fish tissue using size-exclusion chromatography coupled "on-line" with icp-isotope dilution-time-of-flight-mass spectrometry |
publisher |
HAL CCSD |
publishDate |
2003 |
url |
https://hal.science/hal-00291360 https://doi.org/10.1039/b212889f |
genre |
European eel |
genre_facet |
European eel |
op_source |
ISSN: 0003-2654 EISSN: 1364-5528 Analyst https://hal.science/hal-00291360 Analyst, 2003, 128 (6), pp.651-657. ⟨10.1039/b212889f⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1039/b212889f hal-00291360 https://hal.science/hal-00291360 doi:10.1039/b212889f |
op_doi |
https://doi.org/10.1039/b212889f |
container_title |
The Analyst |
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128 |
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6 |
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651 |
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657 |
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1782333879702519808 |