Functional characterisation of the haemoglobins of the migratory notothenioid fish Dissostichus eleginoides
This study addresses the primary structure, the oxygen-binding properties and the CO-rebinding kinetics of the haemoglobins of the Patagonian toothfish Dissostichus eleginoides. D. eleginoides belongs to the family Nototheniidae, the most diversified of the suborder Notothenioidei, mostly exhibiting...
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ftunivparmairis:oai:air.unipr.it:11381/2805892 2024-04-21T07:47:20+00:00 Functional characterisation of the haemoglobins of the migratory notothenioid fish Dissostichus eleginoides Coppola, Daniela Giordano, Daniela Marchesani, Francesco Balestrieri, Marco di Prisco, Guido Verde, Cinzia ABBRUZZETTI, Stefania VIAPPIANI, Cristiano BRUNO, Stefano Coppola, Daniela Giordano, Daniela Abbruzzetti, Stefania Marchesani, Francesco Balestrieri, Marco di Prisco, Guido Viappiani, Cristiano Bruno, Stefano Verde, Cinzia 2015 http://hdl.handle.net/11381/2805892 https://doi.org/10.1007/s10750-015-2439-2 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000362964400019 volume:761 firstpage:315 lastpage:333 numberofpages:19 journal:HYDROBIOLOGIA http://hdl.handle.net/11381/2805892 doi:10.1007/s10750-015-2439-2 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84944355933 info:eu-repo/semantics/article 2015 ftunivparmairis https://doi.org/10.1007/s10750-015-2439-2 2024-03-28T01:18:34Z This study addresses the primary structure, the oxygen-binding properties and the CO-rebinding kinetics of the haemoglobins of the Patagonian toothfish Dissostichus eleginoides. D. eleginoides belongs to the family Nototheniidae, the most diversified of the suborder Notothenioidei, mostly exhibiting an Antarctic distribution. Some of its features are typical of Antarctic species, some are not. For instance, D. eleginoides appears not to have functional antifreeze glycoproteins (consistent with its non- Antarctic distribution). In contrast, it has a major and a minor haemoglobin (similar to many Antarctic notothenioids), and their very low oxygen affinity does not follow the trend of other non-Antarctic notothenioids and appears typical of cold-adapted species. Moreover, the amino-acid sequence reveals high identity with the globins of Antarctic notothenioids, arguing in favour of a common origin within notothenioids, and indicates that the primary structure of the major and minor haemoglobins has undergone modifications only to a limited extent. The ligand-rebinding kinetics of the major haemoglobin of D. eleginoides indicate a strong stabilisation of the quaternary T state at lower pH values. Article in Journal/Newspaper Antarc* Antarctic Patagonian Toothfish Archivio della ricerca dell'Università di Parma (CINECA IRIS) Hydrobiologia 761 1 315 333 |
institution |
Open Polar |
collection |
Archivio della ricerca dell'Università di Parma (CINECA IRIS) |
op_collection_id |
ftunivparmairis |
language |
English |
description |
This study addresses the primary structure, the oxygen-binding properties and the CO-rebinding kinetics of the haemoglobins of the Patagonian toothfish Dissostichus eleginoides. D. eleginoides belongs to the family Nototheniidae, the most diversified of the suborder Notothenioidei, mostly exhibiting an Antarctic distribution. Some of its features are typical of Antarctic species, some are not. For instance, D. eleginoides appears not to have functional antifreeze glycoproteins (consistent with its non- Antarctic distribution). In contrast, it has a major and a minor haemoglobin (similar to many Antarctic notothenioids), and their very low oxygen affinity does not follow the trend of other non-Antarctic notothenioids and appears typical of cold-adapted species. Moreover, the amino-acid sequence reveals high identity with the globins of Antarctic notothenioids, arguing in favour of a common origin within notothenioids, and indicates that the primary structure of the major and minor haemoglobins has undergone modifications only to a limited extent. The ligand-rebinding kinetics of the major haemoglobin of D. eleginoides indicate a strong stabilisation of the quaternary T state at lower pH values. |
author2 |
Coppola, Daniela Giordano, Daniela Abbruzzetti, Stefania Marchesani, Francesco Balestrieri, Marco di Prisco, Guido Viappiani, Cristiano Bruno, Stefano Verde, Cinzia |
format |
Article in Journal/Newspaper |
author |
Coppola, Daniela Giordano, Daniela Marchesani, Francesco Balestrieri, Marco di Prisco, Guido Verde, Cinzia ABBRUZZETTI, Stefania VIAPPIANI, Cristiano BRUNO, Stefano |
spellingShingle |
Coppola, Daniela Giordano, Daniela Marchesani, Francesco Balestrieri, Marco di Prisco, Guido Verde, Cinzia ABBRUZZETTI, Stefania VIAPPIANI, Cristiano BRUNO, Stefano Functional characterisation of the haemoglobins of the migratory notothenioid fish Dissostichus eleginoides |
author_facet |
Coppola, Daniela Giordano, Daniela Marchesani, Francesco Balestrieri, Marco di Prisco, Guido Verde, Cinzia ABBRUZZETTI, Stefania VIAPPIANI, Cristiano BRUNO, Stefano |
author_sort |
Coppola, Daniela |
title |
Functional characterisation of the haemoglobins of the migratory notothenioid fish Dissostichus eleginoides |
title_short |
Functional characterisation of the haemoglobins of the migratory notothenioid fish Dissostichus eleginoides |
title_full |
Functional characterisation of the haemoglobins of the migratory notothenioid fish Dissostichus eleginoides |
title_fullStr |
Functional characterisation of the haemoglobins of the migratory notothenioid fish Dissostichus eleginoides |
title_full_unstemmed |
Functional characterisation of the haemoglobins of the migratory notothenioid fish Dissostichus eleginoides |
title_sort |
functional characterisation of the haemoglobins of the migratory notothenioid fish dissostichus eleginoides |
publishDate |
2015 |
url |
http://hdl.handle.net/11381/2805892 https://doi.org/10.1007/s10750-015-2439-2 |
genre |
Antarc* Antarctic Patagonian Toothfish |
genre_facet |
Antarc* Antarctic Patagonian Toothfish |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000362964400019 volume:761 firstpage:315 lastpage:333 numberofpages:19 journal:HYDROBIOLOGIA http://hdl.handle.net/11381/2805892 doi:10.1007/s10750-015-2439-2 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84944355933 |
op_doi |
https://doi.org/10.1007/s10750-015-2439-2 |
container_title |
Hydrobiologia |
container_volume |
761 |
container_issue |
1 |
container_start_page |
315 |
op_container_end_page |
333 |
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1796946192860446720 |