Midichloria mitochondrii is widespread in hard ticks (Ixodidae) and resides in the mitochondria of phylogenetically diverse species
The hard tick Ixodes ricinus (Ixodidae) is the sole animal thus far shown to harbour an intra-mitochondrial bacterium, which has recently been named Midichloria mitochondrii. The objectives of this work were (i) to screen ixodid ticks for Midichloria-related bacteria and (ii) to determine whether th...
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Online Access: | http://hdl.handle.net/11381/2797430 https://doi.org/10.1017/S0031182007004052 |
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ftunivparmairis:oai:air.unipr.it:11381/2797430 2024-04-14T08:13:48+00:00 Midichloria mitochondrii is widespread in hard ticks (Ixodidae) and resides in the mitochondria of phylogenetically diverse species S. Epis D. Sassera T. Beninati N. Lo L. Beati J. Piesman L. Rinaldi K. D. McCoy A. Torina L. Sacchi E. Clementi S. Magnino C. Bandi GENCHI, Marco S., Epi D., Sassera T., Beninati N., Lo L., Beati J., Piesman L., Rinaldi K. D., Mccoy A., Torina L., Sacchi E., Clementi Genchi, Marco S., Magnino C., Bandi 2008 http://hdl.handle.net/11381/2797430 https://doi.org/10.1017/S0031182007004052 eng eng info:eu-repo/semantics/altIdentifier/pmid/18205982 info:eu-repo/semantics/altIdentifier/wos/WOS:000255619700008 volume:135 issue:4 firstpage:485 lastpage:494 numberofpages:10 journal:PARASITOLOGY http://hdl.handle.net/11381/2797430 doi:10.1017/S0031182007004052 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-43249124717 Hard tick Intra-mitochondrial Ixodes ricinu Midichloria mitochondrii PCR screening Phylogeny info:eu-repo/semantics/article 2008 ftunivparmairis https://doi.org/10.1017/S0031182007004052 2024-03-21T18:23:30Z The hard tick Ixodes ricinus (Ixodidae) is the sole animal thus far shown to harbour an intra-mitochondrial bacterium, which has recently been named Midichloria mitochondrii. The objectives of this work were (i) to screen ixodid ticks for Midichloria-related bacteria and (ii) to determine whether these bacteria exploit the intra-mitochondrial niche in other tick species. Our main goal was to discover further models of this peculiar form of symbiosis. We have thus performed a PCR screening for Midichloria-related bacteria in samples of ixodid ticks collected in Italy, North America and Iceland. A total of 7 newly examined species from 5 genera were found positive for bacteria closely related to M. mitochondrii. Samples of the tick species Rhipicephalus bursa, found positive in the PCR screening, were analysed with transmission electron microscopy, which revealed the presence of bacteria both in the cytoplasm and in the mitochondria of the oocytes. There is thus evidence that bacteria invade mitochondria in at least 2 tick species. Phylogenetic analysis on the bacterial 16S rRNA gene sequences generated from positive specimens revealed that the bacteria form a monophyletic group within the order Rickettsiales. The phylogeny of Midichloria symbionts and related bacteria does not appear completely congruent with the phylogeny of the hosts. Article in Journal/Newspaper Iceland Archivio della ricerca dell'Università di Parma (CINECA IRIS) Parasitology 135 4 485 494 |
institution |
Open Polar |
collection |
Archivio della ricerca dell'Università di Parma (CINECA IRIS) |
op_collection_id |
ftunivparmairis |
language |
English |
topic |
Hard tick Intra-mitochondrial Ixodes ricinu Midichloria mitochondrii PCR screening Phylogeny |
spellingShingle |
Hard tick Intra-mitochondrial Ixodes ricinu Midichloria mitochondrii PCR screening Phylogeny S. Epis D. Sassera T. Beninati N. Lo L. Beati J. Piesman L. Rinaldi K. D. McCoy A. Torina L. Sacchi E. Clementi S. Magnino C. Bandi GENCHI, Marco Midichloria mitochondrii is widespread in hard ticks (Ixodidae) and resides in the mitochondria of phylogenetically diverse species |
topic_facet |
Hard tick Intra-mitochondrial Ixodes ricinu Midichloria mitochondrii PCR screening Phylogeny |
description |
The hard tick Ixodes ricinus (Ixodidae) is the sole animal thus far shown to harbour an intra-mitochondrial bacterium, which has recently been named Midichloria mitochondrii. The objectives of this work were (i) to screen ixodid ticks for Midichloria-related bacteria and (ii) to determine whether these bacteria exploit the intra-mitochondrial niche in other tick species. Our main goal was to discover further models of this peculiar form of symbiosis. We have thus performed a PCR screening for Midichloria-related bacteria in samples of ixodid ticks collected in Italy, North America and Iceland. A total of 7 newly examined species from 5 genera were found positive for bacteria closely related to M. mitochondrii. Samples of the tick species Rhipicephalus bursa, found positive in the PCR screening, were analysed with transmission electron microscopy, which revealed the presence of bacteria both in the cytoplasm and in the mitochondria of the oocytes. There is thus evidence that bacteria invade mitochondria in at least 2 tick species. Phylogenetic analysis on the bacterial 16S rRNA gene sequences generated from positive specimens revealed that the bacteria form a monophyletic group within the order Rickettsiales. The phylogeny of Midichloria symbionts and related bacteria does not appear completely congruent with the phylogeny of the hosts. |
author2 |
S., Epi D., Sassera T., Beninati N., Lo L., Beati J., Piesman L., Rinaldi K. D., Mccoy A., Torina L., Sacchi E., Clementi Genchi, Marco S., Magnino C., Bandi |
format |
Article in Journal/Newspaper |
author |
S. Epis D. Sassera T. Beninati N. Lo L. Beati J. Piesman L. Rinaldi K. D. McCoy A. Torina L. Sacchi E. Clementi S. Magnino C. Bandi GENCHI, Marco |
author_facet |
S. Epis D. Sassera T. Beninati N. Lo L. Beati J. Piesman L. Rinaldi K. D. McCoy A. Torina L. Sacchi E. Clementi S. Magnino C. Bandi GENCHI, Marco |
author_sort |
S. Epis |
title |
Midichloria mitochondrii is widespread in hard ticks (Ixodidae) and resides in the mitochondria of phylogenetically diverse species |
title_short |
Midichloria mitochondrii is widespread in hard ticks (Ixodidae) and resides in the mitochondria of phylogenetically diverse species |
title_full |
Midichloria mitochondrii is widespread in hard ticks (Ixodidae) and resides in the mitochondria of phylogenetically diverse species |
title_fullStr |
Midichloria mitochondrii is widespread in hard ticks (Ixodidae) and resides in the mitochondria of phylogenetically diverse species |
title_full_unstemmed |
Midichloria mitochondrii is widespread in hard ticks (Ixodidae) and resides in the mitochondria of phylogenetically diverse species |
title_sort |
midichloria mitochondrii is widespread in hard ticks (ixodidae) and resides in the mitochondria of phylogenetically diverse species |
publishDate |
2008 |
url |
http://hdl.handle.net/11381/2797430 https://doi.org/10.1017/S0031182007004052 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/18205982 info:eu-repo/semantics/altIdentifier/wos/WOS:000255619700008 volume:135 issue:4 firstpage:485 lastpage:494 numberofpages:10 journal:PARASITOLOGY http://hdl.handle.net/11381/2797430 doi:10.1017/S0031182007004052 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-43249124717 |
op_doi |
https://doi.org/10.1017/S0031182007004052 |
container_title |
Parasitology |
container_volume |
135 |
container_issue |
4 |
container_start_page |
485 |
op_container_end_page |
494 |
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1796311874297397248 |