Structural characterization of recombinant crustacyanin subunits from the lobster Homarus americanus.

Crustacean crustacyanin proteins are linked to the production and modification of carapace colour, with direct implications for fitness and survival. Here, the structural and functional properties of the two recombinant crustacyanin subunits H1 and H2 from the American lobster Homarus americanus are...

Full description

Bibliographic Details
Published in:Acta Crystallographica Section F Structural Biology and Crystallization Communications
Main Authors: M. Ferrari, E. Pincolini, T. S. McClintock, M. Rossle, M. Cianci, FOLLI, Claudia, BERNI, Rodolfo
Other Authors: M., Ferrari, Folli, Claudia, E., Pincolini, T. S., Mcclintock, M., Rossle, Berni, Rodolfo, M., Cianci
Format: Article in Journal/Newspaper
Language:English
Published: 2012
Subjects:
European lobster
Online Access:http://hdl.handle.net/11381/2509045
https://doi.org/10.1107/S1744309112026103
http://scripts.iucr.org/cgi-bin/paper?S1744309112026103
Description
Summary:Crustacean crustacyanin proteins are linked to the production and modification of carapace colour, with direct implications for fitness and survival. Here, the structural and functional properties of the two recombinant crustacyanin subunits H1 and H2 from the American lobster Homarus americanus are reported. The two subunits are structurally highly similar to the corresponding natural apo crustacyanin CRTC and CRTA subunits from the European lobster H. gammarus. Reconstitution studies of the recombinant crustacyanin proteins H1 and H2 with astaxanthin reproduced the bathochromic shift of 85-95 nm typical of the natural crustacyanin subunits from H. gammarus in complex with astaxanthin. Moreover, correlations between the presence of crustacyanin genes in crustacean species and the resulting carapace colours with the spectral properties of the subunits in complex with astaxanthin confirmed this genotype-phenotype linkage.

Similar Items