Shell extracts of the edible mussel and oyster induce an enhancement of the catabolic pathway of human skin fibroblasts, in vitro
15 pages International audience Mollusc shells are composed of more than 95% calcium carbonate and less than 5% organic matrix consisting mostly of proteins, glycoproteins and polysaccharides. In this study, we investigated the effects of matrix macromolecular components extracted from the shells of...
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Online Access: | https://hal.science/hal-01588926 https://doi.org/10.1007/s10616-017-0096-1 |
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ftunivparisseine:oai:HAL:hal-01588926v1 2024-04-14T08:10:43+00:00 Shell extracts of the edible mussel and oyster induce an enhancement of the catabolic pathway of human skin fibroblasts, in vitro Latire, Thomas Legendre, Florence Bouyoucef, Mouloud Marin, Frédéric Carreiras, Franck Rigot-Jolivet, Muriel Lebel, Jean-Marc Galéra, Philippe Serpentini, Antoine Université de Caen Normandie (UNICAEN) Normandie Université (NU) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Microenvironnement cellulaire et pathologie (MILPAT) Normandie Université (NU)-Normandie Université (NU) Biogéosciences UMR 6282 (BGS) Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS) Equipe de recherche sur les relations matrice extracellulaire-cellules (ERRMECe) Fédération INSTITUT DES MATÉRIAUX DE CERGY-PONTOISE (I-MAT) Université de Cergy Pontoise (UCP) Université Paris-Seine-Université Paris-Seine-Université de Cergy Pontoise (UCP) Université Paris-Seine-Université Paris-Seine Department of Plastic and Reconstructive Surgery St-Martin Clinic Work financially supported by the ‘‘Fonds Unique Interministériel’’ (FUI, French ministry of Economy and Industry, SEMINEROIL program) 09 2 90 6042 , and by a PhD fellowship from the FUI and the Conseil Régional de Basse-Normandie 917RB103 . 2017-10 https://hal.science/hal-01588926 https://doi.org/10.1007/s10616-017-0096-1 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s10616-017-0096-1 info:eu-repo/semantics/altIdentifier/pmid/28474214 hal-01588926 https://hal.science/hal-01588926 doi:10.1007/s10616-017-0096-1 PUBMED: 28474214 PUBMEDCENTRAL: PMC5595752 ISSN: 0920-9069 EISSN: 1573-0778 Cytotechnology https://hal.science/hal-01588926 Cytotechnology, 2017, 69 (5), pp.815-829. ⟨10.1007/s10616-017-0096-1⟩ https://link.springer.com/article/10.1007/s10616-017-0096-1 Biological activity Extracellular matrix Fibroblast Mollusc Shell matrix [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology info:eu-repo/semantics/article Journal articles 2017 ftunivparisseine https://doi.org/10.1007/s10616-017-0096-1 2024-03-21T15:26:38Z 15 pages International audience Mollusc shells are composed of more than 95% calcium carbonate and less than 5% organic matrix consisting mostly of proteins, glycoproteins and polysaccharides. In this study, we investigated the effects of matrix macromolecular components extracted from the shells of two edible molluscs of economic interest, i.e., the blue mussel Mytilus edulis and the Pacific oyster Crassostrea gigas. The potential biological activities of these organic molecules were analysed on human dermal fibroblasts in primary culture. Our results demonstrate that shell extracts of the two studied molluscs modulate the metabolic activities of the cells. In addition, the extracts caused a decrease of type I collagen and a concomitant increase of active MMP-1, both at the mRNA and the protein levels. Therefore, our results suggest that shell extracts from M. edulis and C. gigas contain molecules that promote the catabolic pathway of human dermal fibroblasts. This work emphasises the potential use of these shell matrices in the context of anti-fibrotic strategies, particularly against scleroderma. More generally, it stresses the usefulness to valorise bivalve shells that are coproducts of shellfish farming activity. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université Paris Seine: ComUE (HAL) Pacific Cytotechnology 69 5 815 829 |
institution |
Open Polar |
collection |
Université Paris Seine: ComUE (HAL) |
op_collection_id |
ftunivparisseine |
language |
English |
topic |
Biological activity Extracellular matrix Fibroblast Mollusc Shell matrix [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology |
spellingShingle |
Biological activity Extracellular matrix Fibroblast Mollusc Shell matrix [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Latire, Thomas Legendre, Florence Bouyoucef, Mouloud Marin, Frédéric Carreiras, Franck Rigot-Jolivet, Muriel Lebel, Jean-Marc Galéra, Philippe Serpentini, Antoine Shell extracts of the edible mussel and oyster induce an enhancement of the catabolic pathway of human skin fibroblasts, in vitro |
topic_facet |
Biological activity Extracellular matrix Fibroblast Mollusc Shell matrix [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology |
description |
15 pages International audience Mollusc shells are composed of more than 95% calcium carbonate and less than 5% organic matrix consisting mostly of proteins, glycoproteins and polysaccharides. In this study, we investigated the effects of matrix macromolecular components extracted from the shells of two edible molluscs of economic interest, i.e., the blue mussel Mytilus edulis and the Pacific oyster Crassostrea gigas. The potential biological activities of these organic molecules were analysed on human dermal fibroblasts in primary culture. Our results demonstrate that shell extracts of the two studied molluscs modulate the metabolic activities of the cells. In addition, the extracts caused a decrease of type I collagen and a concomitant increase of active MMP-1, both at the mRNA and the protein levels. Therefore, our results suggest that shell extracts from M. edulis and C. gigas contain molecules that promote the catabolic pathway of human dermal fibroblasts. This work emphasises the potential use of these shell matrices in the context of anti-fibrotic strategies, particularly against scleroderma. More generally, it stresses the usefulness to valorise bivalve shells that are coproducts of shellfish farming activity. |
author2 |
Université de Caen Normandie (UNICAEN) Normandie Université (NU) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Microenvironnement cellulaire et pathologie (MILPAT) Normandie Université (NU)-Normandie Université (NU) Biogéosciences UMR 6282 (BGS) Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS) Equipe de recherche sur les relations matrice extracellulaire-cellules (ERRMECe) Fédération INSTITUT DES MATÉRIAUX DE CERGY-PONTOISE (I-MAT) Université de Cergy Pontoise (UCP) Université Paris-Seine-Université Paris-Seine-Université de Cergy Pontoise (UCP) Université Paris-Seine-Université Paris-Seine Department of Plastic and Reconstructive Surgery St-Martin Clinic Work financially supported by the ‘‘Fonds Unique Interministériel’’ (FUI, French ministry of Economy and Industry, SEMINEROIL program) 09 2 90 6042 , and by a PhD fellowship from the FUI and the Conseil Régional de Basse-Normandie 917RB103 . |
format |
Article in Journal/Newspaper |
author |
Latire, Thomas Legendre, Florence Bouyoucef, Mouloud Marin, Frédéric Carreiras, Franck Rigot-Jolivet, Muriel Lebel, Jean-Marc Galéra, Philippe Serpentini, Antoine |
author_facet |
Latire, Thomas Legendre, Florence Bouyoucef, Mouloud Marin, Frédéric Carreiras, Franck Rigot-Jolivet, Muriel Lebel, Jean-Marc Galéra, Philippe Serpentini, Antoine |
author_sort |
Latire, Thomas |
title |
Shell extracts of the edible mussel and oyster induce an enhancement of the catabolic pathway of human skin fibroblasts, in vitro |
title_short |
Shell extracts of the edible mussel and oyster induce an enhancement of the catabolic pathway of human skin fibroblasts, in vitro |
title_full |
Shell extracts of the edible mussel and oyster induce an enhancement of the catabolic pathway of human skin fibroblasts, in vitro |
title_fullStr |
Shell extracts of the edible mussel and oyster induce an enhancement of the catabolic pathway of human skin fibroblasts, in vitro |
title_full_unstemmed |
Shell extracts of the edible mussel and oyster induce an enhancement of the catabolic pathway of human skin fibroblasts, in vitro |
title_sort |
shell extracts of the edible mussel and oyster induce an enhancement of the catabolic pathway of human skin fibroblasts, in vitro |
publisher |
HAL CCSD |
publishDate |
2017 |
url |
https://hal.science/hal-01588926 https://doi.org/10.1007/s10616-017-0096-1 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0920-9069 EISSN: 1573-0778 Cytotechnology https://hal.science/hal-01588926 Cytotechnology, 2017, 69 (5), pp.815-829. ⟨10.1007/s10616-017-0096-1⟩ https://link.springer.com/article/10.1007/s10616-017-0096-1 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10616-017-0096-1 info:eu-repo/semantics/altIdentifier/pmid/28474214 hal-01588926 https://hal.science/hal-01588926 doi:10.1007/s10616-017-0096-1 PUBMED: 28474214 PUBMEDCENTRAL: PMC5595752 |
op_doi |
https://doi.org/10.1007/s10616-017-0096-1 |
container_title |
Cytotechnology |
container_volume |
69 |
container_issue |
5 |
container_start_page |
815 |
op_container_end_page |
829 |
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1796308367624372224 |