Role of mucosal immune response and histopathological study in European eel (Anguilla anguilla L.) intraperitoneal challenged by Vibrio anguillarum or Tenacibaculum soleae
The external mucus layer that covers fish skin contains numerous immune substances scarcely studied that act as the first line of defence against a broad spectrum of pathogens. This study aimed to characterize and describe for the first time several humoral immune defence parameters in the skin mucu...
Published in: | Fish & Shellfish Immunology |
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2021
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Online Access: | http://hdl.handle.net/10447/547605 https://doi.org/10.1016/j.fsi.2021.05.011 |
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ftunivpalermo:oai:iris.unipa.it:10447/547605 2024-02-11T09:55:27+01:00 Role of mucosal immune response and histopathological study in European eel (Anguilla anguilla L.) intraperitoneal challenged by Vibrio anguillarum or Tenacibaculum soleae Conforto E. Vilchez-Gomez L. Parrinello D. Parisi M. G. Esteban M. A. Cammarata M. Guardiola F. A. Conforto E. Vilchez-Gomez L. Parrinello D. Parisi M.G. Esteban M.A. Cammarata M. Guardiola F.A. 2021-05-18 http://hdl.handle.net/10447/547605 https://doi.org/10.1016/j.fsi.2021.05.011 eng eng Academic Press info:eu-repo/semantics/altIdentifier/pmid/34015481 info:eu-repo/semantics/altIdentifier/wos/WOS:000657390500007 volume:114 firstpage:330 lastpage:339 numberofpages:10 journal:FISH AND SHELLFISH IMMUNOLOGY http://hdl.handle.net/10447/547605 doi:10.1016/j.fsi.2021.05.011 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85107061628 info:eu-repo/semantics/closedAccess Mucosal immunity European eel (Anguilla Anguilla L.) Immunity Mucosal Bacterial challenge Settore BIO/05 - Zoologia info:eu-repo/semantics/article 2021 ftunivpalermo https://doi.org/10.1016/j.fsi.2021.05.011 2024-01-23T23:32:37Z The external mucus layer that covers fish skin contains numerous immune substances scarcely studied that act as the first line of defence against a broad spectrum of pathogens. This study aimed to characterize and describe for the first time several humoral immune defence parameters in the skin mucus of the European eel (Anguilla anguilla) after intraperitoneal injection with Vibrio anguillarum or Tenacibaculum soleae. This study evaluated several immune-related enzymes and bactericidal activity against fish pathogenic bacteria in the skin mucus of European eels at 24, 48, and 72 h post-challenge. The results demonstrated that European eel skin mucus showed significant increments in peroxidase and lysozyme activity at 48 and 72 h after V. anguillarum challenge, compared to other experimental groups. In the case of antiprotease activity, an increase was observed at 24 h in the skin mucus of fish challenged with V. anguillarum compared to unchallenged fish, while this activity was undetected at 48 and 72 h. In contrast, protease activity had decreased at 48 and 72 h in the skin mucus of fish challenged with V. anguillarum compared to the unchallenged group. Regarding bactericidal activity, a high growth capacity of T. soleae was observed in the skin mucus of all experimental groups. Interestingly, the skin mucus from fish challenged with V. anguillarum exhibited increased bactericidal activity against this bacterium at 48 h, compared to unchallenged fish. Finally, severe histopathological alterations were observed in the gills and liver at the end of the trial (72 h), whereas the skin showed only an overspread presence of goblet cells in the challenged fish compared to unchallenged fish. The present results may give new insights into the mucosal immune system of this primitive species with potential applications in aquaculture. Article in Journal/Newspaper Anguilla anguilla European eel IRIS Università degli Studi di Palermo Fish & Shellfish Immunology 114 330 339 |
institution |
Open Polar |
collection |
IRIS Università degli Studi di Palermo |
op_collection_id |
ftunivpalermo |
language |
English |
topic |
Mucosal immunity European eel (Anguilla Anguilla L.) Immunity Mucosal Bacterial challenge Settore BIO/05 - Zoologia |
spellingShingle |
Mucosal immunity European eel (Anguilla Anguilla L.) Immunity Mucosal Bacterial challenge Settore BIO/05 - Zoologia Conforto E. Vilchez-Gomez L. Parrinello D. Parisi M. G. Esteban M. A. Cammarata M. Guardiola F. A. Role of mucosal immune response and histopathological study in European eel (Anguilla anguilla L.) intraperitoneal challenged by Vibrio anguillarum or Tenacibaculum soleae |
topic_facet |
Mucosal immunity European eel (Anguilla Anguilla L.) Immunity Mucosal Bacterial challenge Settore BIO/05 - Zoologia |
description |
The external mucus layer that covers fish skin contains numerous immune substances scarcely studied that act as the first line of defence against a broad spectrum of pathogens. This study aimed to characterize and describe for the first time several humoral immune defence parameters in the skin mucus of the European eel (Anguilla anguilla) after intraperitoneal injection with Vibrio anguillarum or Tenacibaculum soleae. This study evaluated several immune-related enzymes and bactericidal activity against fish pathogenic bacteria in the skin mucus of European eels at 24, 48, and 72 h post-challenge. The results demonstrated that European eel skin mucus showed significant increments in peroxidase and lysozyme activity at 48 and 72 h after V. anguillarum challenge, compared to other experimental groups. In the case of antiprotease activity, an increase was observed at 24 h in the skin mucus of fish challenged with V. anguillarum compared to unchallenged fish, while this activity was undetected at 48 and 72 h. In contrast, protease activity had decreased at 48 and 72 h in the skin mucus of fish challenged with V. anguillarum compared to the unchallenged group. Regarding bactericidal activity, a high growth capacity of T. soleae was observed in the skin mucus of all experimental groups. Interestingly, the skin mucus from fish challenged with V. anguillarum exhibited increased bactericidal activity against this bacterium at 48 h, compared to unchallenged fish. Finally, severe histopathological alterations were observed in the gills and liver at the end of the trial (72 h), whereas the skin showed only an overspread presence of goblet cells in the challenged fish compared to unchallenged fish. The present results may give new insights into the mucosal immune system of this primitive species with potential applications in aquaculture. |
author2 |
Conforto E. Vilchez-Gomez L. Parrinello D. Parisi M.G. Esteban M.A. Cammarata M. Guardiola F.A. |
format |
Article in Journal/Newspaper |
author |
Conforto E. Vilchez-Gomez L. Parrinello D. Parisi M. G. Esteban M. A. Cammarata M. Guardiola F. A. |
author_facet |
Conforto E. Vilchez-Gomez L. Parrinello D. Parisi M. G. Esteban M. A. Cammarata M. Guardiola F. A. |
author_sort |
Conforto E. |
title |
Role of mucosal immune response and histopathological study in European eel (Anguilla anguilla L.) intraperitoneal challenged by Vibrio anguillarum or Tenacibaculum soleae |
title_short |
Role of mucosal immune response and histopathological study in European eel (Anguilla anguilla L.) intraperitoneal challenged by Vibrio anguillarum or Tenacibaculum soleae |
title_full |
Role of mucosal immune response and histopathological study in European eel (Anguilla anguilla L.) intraperitoneal challenged by Vibrio anguillarum or Tenacibaculum soleae |
title_fullStr |
Role of mucosal immune response and histopathological study in European eel (Anguilla anguilla L.) intraperitoneal challenged by Vibrio anguillarum or Tenacibaculum soleae |
title_full_unstemmed |
Role of mucosal immune response and histopathological study in European eel (Anguilla anguilla L.) intraperitoneal challenged by Vibrio anguillarum or Tenacibaculum soleae |
title_sort |
role of mucosal immune response and histopathological study in european eel (anguilla anguilla l.) intraperitoneal challenged by vibrio anguillarum or tenacibaculum soleae |
publisher |
Academic Press |
publishDate |
2021 |
url |
http://hdl.handle.net/10447/547605 https://doi.org/10.1016/j.fsi.2021.05.011 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/34015481 info:eu-repo/semantics/altIdentifier/wos/WOS:000657390500007 volume:114 firstpage:330 lastpage:339 numberofpages:10 journal:FISH AND SHELLFISH IMMUNOLOGY http://hdl.handle.net/10447/547605 doi:10.1016/j.fsi.2021.05.011 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85107061628 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.fsi.2021.05.011 |
container_title |
Fish & Shellfish Immunology |
container_volume |
114 |
container_start_page |
330 |
op_container_end_page |
339 |
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