Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus
The endocannabinoid system modulates synaptic plasticity in the hippocampus, but a link between long-term synaptic plasticity and the type 1 cannabinoid (CB1) receptor at medial perforant path (MPP) synapses remains elusive. Here, immuno-electron microscopy in adult mice showed that similar to 26% o...
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Online Access: | http://hdl.handle.net/10810/41425 https://doi.org/10.1016/j.neuropharm.2019.04.020 |
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ftunivpaisvasco:oai:addi.ehu.es:10810/41425 2023-05-15T16:01:53+02:00 Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus Peñasco Iglesias, Sara Rico Barrio, Irantzu Puente Bustinza, Nagore Gómez Urquijo, Sonia María Fontaine, Christine J. Egaña Huguet, Jon Achicallende Urcaregui, Svein Ramos Uriarte, Almudena Reguero Acebal, Leire Elezgarai Gabantxo, Izaskun Nahirney, Patrick C. Christie, Brian R. Grandes Moreno, Pedro Rolando 2019-04-22 http://hdl.handle.net/10810/41425 https://doi.org/10.1016/j.neuropharm.2019.04.020 eng eng Elsevier info:eu-repo/grantAgreement/MINECO/SAF2015-65034-R info:eu-repo/grantAgreement/MINECO/BES-2013-065057 https://www.sciencedirect.com/science/article/pii/S0028390819301364?via%3Dihub Neuropharmacology 153 : 32-40 (2019) 0028-3908 1873-7064 http://hdl.handle.net/10810/41425 doi:10.1016/j.neuropharm.2019.04.020 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/3.0/es/ © 2019 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/). Atribución-NoComercial-SinDerivadas 3.0 España CC-BY-NC-ND cb receptor 2-ag electrophysiology excitatory synapses long-term depression hippocampus cannabinoid receptor plasticity system activation memory nmda cb1 inhibition object info:eu-repo/semantics/article 2019 ftunivpaisvasco https://doi.org/10.1016/j.neuropharm.2019.04.020 2022-03-10T16:38:13Z The endocannabinoid system modulates synaptic plasticity in the hippocampus, but a link between long-term synaptic plasticity and the type 1 cannabinoid (CB1) receptor at medial perforant path (MPP) synapses remains elusive. Here, immuno-electron microscopy in adult mice showed that similar to 26% of the excitatory synaptic terminals in the middle 1/3 of the dentate molecular layer (DML) contained CB1 receptors, and field excitatory postsynaptic potentials evoked by MPP stimulation were inhibited by CB1 receptor activation. In addition, MPP stimulation at 10 Hz for 10 min triggered CB, receptor-dependent excitatory long-term depression (eCB-eLTD) at MPP synapses of wild-type mice but not on CB1-knockout mice. This eCB-eLTD was group I mGluR-dependent, required intracellular calcium influx and 2-arachydonoyl-glycerol (2-AG) synthesis but did not depend on N-methyl-d-aspartate (NMDA) receptors. Overall, these results point to a functional role for CB1 receptors with eCB-eLTD at DML MPP synapses and further involve these receptors in memory processing within the adult brain. We thank all members of P. Grandes laboratory for their helpful comments, suggestions, and discussions during the performance of this study. The authors thank Giovanni Marsicano (INSERM, U1215 Neurocentre Magendie, Endocannabinoids and Neuroadaptation, Bordeaux, France. University de Bordeaux, France), Beat Lutz (Institute of Physiological Chemistry and German Resilience Center, University Medical Center of the Johannes Gutenberg University Mainz, Germany) and Susana Mato (Achucarro Basque Center for Neuroscience, Science Park of the UPV/EHU, Leioa, Vizcaya, Spain) for providing the CB 1 receptor knock-out mice. This work was supported by MINECO/FEDER, UE (grant number SAF2015-65034-R to PG); The Basque Government (grant number BCG IT764-13 to PG); Red de Trastornos Adictivos, Instituto de Salud Carlos III (ISC-III) and European Regional Development Funds-European Union (ERDF-EU; grant RD16/0017/0012 to PG); PhD contract from MINECO ... Article in Journal/Newspaper DML ADDI: Repositorio Institucional de la Universidad del País Vasco (UPV) Neuropharmacology 153 32 40 |
institution |
Open Polar |
collection |
ADDI: Repositorio Institucional de la Universidad del País Vasco (UPV) |
op_collection_id |
ftunivpaisvasco |
language |
English |
topic |
cb receptor 2-ag electrophysiology excitatory synapses long-term depression hippocampus cannabinoid receptor plasticity system activation memory nmda cb1 inhibition object |
spellingShingle |
cb receptor 2-ag electrophysiology excitatory synapses long-term depression hippocampus cannabinoid receptor plasticity system activation memory nmda cb1 inhibition object Peñasco Iglesias, Sara Rico Barrio, Irantzu Puente Bustinza, Nagore Gómez Urquijo, Sonia María Fontaine, Christine J. Egaña Huguet, Jon Achicallende Urcaregui, Svein Ramos Uriarte, Almudena Reguero Acebal, Leire Elezgarai Gabantxo, Izaskun Nahirney, Patrick C. Christie, Brian R. Grandes Moreno, Pedro Rolando Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus |
topic_facet |
cb receptor 2-ag electrophysiology excitatory synapses long-term depression hippocampus cannabinoid receptor plasticity system activation memory nmda cb1 inhibition object |
description |
The endocannabinoid system modulates synaptic plasticity in the hippocampus, but a link between long-term synaptic plasticity and the type 1 cannabinoid (CB1) receptor at medial perforant path (MPP) synapses remains elusive. Here, immuno-electron microscopy in adult mice showed that similar to 26% of the excitatory synaptic terminals in the middle 1/3 of the dentate molecular layer (DML) contained CB1 receptors, and field excitatory postsynaptic potentials evoked by MPP stimulation were inhibited by CB1 receptor activation. In addition, MPP stimulation at 10 Hz for 10 min triggered CB, receptor-dependent excitatory long-term depression (eCB-eLTD) at MPP synapses of wild-type mice but not on CB1-knockout mice. This eCB-eLTD was group I mGluR-dependent, required intracellular calcium influx and 2-arachydonoyl-glycerol (2-AG) synthesis but did not depend on N-methyl-d-aspartate (NMDA) receptors. Overall, these results point to a functional role for CB1 receptors with eCB-eLTD at DML MPP synapses and further involve these receptors in memory processing within the adult brain. We thank all members of P. Grandes laboratory for their helpful comments, suggestions, and discussions during the performance of this study. The authors thank Giovanni Marsicano (INSERM, U1215 Neurocentre Magendie, Endocannabinoids and Neuroadaptation, Bordeaux, France. University de Bordeaux, France), Beat Lutz (Institute of Physiological Chemistry and German Resilience Center, University Medical Center of the Johannes Gutenberg University Mainz, Germany) and Susana Mato (Achucarro Basque Center for Neuroscience, Science Park of the UPV/EHU, Leioa, Vizcaya, Spain) for providing the CB 1 receptor knock-out mice. This work was supported by MINECO/FEDER, UE (grant number SAF2015-65034-R to PG); The Basque Government (grant number BCG IT764-13 to PG); Red de Trastornos Adictivos, Instituto de Salud Carlos III (ISC-III) and European Regional Development Funds-European Union (ERDF-EU; grant RD16/0017/0012 to PG); PhD contract from MINECO ... |
format |
Article in Journal/Newspaper |
author |
Peñasco Iglesias, Sara Rico Barrio, Irantzu Puente Bustinza, Nagore Gómez Urquijo, Sonia María Fontaine, Christine J. Egaña Huguet, Jon Achicallende Urcaregui, Svein Ramos Uriarte, Almudena Reguero Acebal, Leire Elezgarai Gabantxo, Izaskun Nahirney, Patrick C. Christie, Brian R. Grandes Moreno, Pedro Rolando |
author_facet |
Peñasco Iglesias, Sara Rico Barrio, Irantzu Puente Bustinza, Nagore Gómez Urquijo, Sonia María Fontaine, Christine J. Egaña Huguet, Jon Achicallende Urcaregui, Svein Ramos Uriarte, Almudena Reguero Acebal, Leire Elezgarai Gabantxo, Izaskun Nahirney, Patrick C. Christie, Brian R. Grandes Moreno, Pedro Rolando |
author_sort |
Peñasco Iglesias, Sara |
title |
Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus |
title_short |
Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus |
title_full |
Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus |
title_fullStr |
Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus |
title_full_unstemmed |
Endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus |
title_sort |
endocannabinoid long-term depression revealed at medial perforant path excitatory synapses in the dentate gyrus |
publisher |
Elsevier |
publishDate |
2019 |
url |
http://hdl.handle.net/10810/41425 https://doi.org/10.1016/j.neuropharm.2019.04.020 |
genre |
DML |
genre_facet |
DML |
op_relation |
info:eu-repo/grantAgreement/MINECO/SAF2015-65034-R info:eu-repo/grantAgreement/MINECO/BES-2013-065057 https://www.sciencedirect.com/science/article/pii/S0028390819301364?via%3Dihub Neuropharmacology 153 : 32-40 (2019) 0028-3908 1873-7064 http://hdl.handle.net/10810/41425 doi:10.1016/j.neuropharm.2019.04.020 |
op_rights |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/3.0/es/ © 2019 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/). Atribución-NoComercial-SinDerivadas 3.0 España |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.neuropharm.2019.04.020 |
container_title |
Neuropharmacology |
container_volume |
153 |
container_start_page |
32 |
op_container_end_page |
40 |
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1766397575398686720 |