Appraisal of the Genus Pleurastrum (Chlorophyta) Based on Molecular and Climate Data
Two green microalgal strains, CCCryo 469-16 and CCCryo 470-16, were isolated from samples of Antarctic microflora. Their morphology and 18S rRNA sequences indicated a phylogenetic relationship with strains of the genera Chlorococcum Meneghini, Macrochloris Korshikov, and Pleurastrum Chodat. The last...
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MDPIST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND
2023
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ftunivpadovairis:oai:www.research.unipd.it:11577/3495947 2024-04-14T08:04:20+00:00 Appraisal of the Genus Pleurastrum (Chlorophyta) Based on Molecular and Climate Data Sciuto K. Wolf M. A. Mistri M. Moro I. Sciuto, K. Wolf, M. A. Mistri, M. Moro, I. 2023 ELETTRONICO https://hdl.handle.net/11577/3495947 https://doi.org/10.3390/d15050650 eng eng MDPIST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND info:eu-repo/semantics/altIdentifier/wos/WOS:000997228900001 volume:15 issue:5 firstpage:1 lastpage:1 numberofpages:1 journal:DIVERSITY https://hdl.handle.net/11577/3495947 doi:10.3390/d15050650 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85160678185 18S rRNA Chlorococcum climate zones ITS2 secondary structure Macrochloris rubrioleum Pleurastrum rbcL tufA info:eu-repo/semantics/article 2023 ftunivpadovairis https://doi.org/10.3390/d15050650 2024-03-21T18:47:41Z Two green microalgal strains, CCCryo 469-16 and CCCryo 470-16, were isolated from samples of Antarctic microflora. Their morphology and 18S rRNA sequences indicated a phylogenetic relationship with strains of the genera Chlorococcum Meneghini, Macrochloris Korshikov, and Pleurastrum Chodat. The last genus is considered particularly problematic as it underwent several revisions. Moreover, its type strain, P. insigne SAG 30.93, was recently demonstrated to coincide genetically with the authentic strain of Chlorococcum oleofaciens from an 18S rRNA phylogeny. This deprived the genus Pleurastrum of an important type reference. Thus, the molecular markers rbcL, tufA, and ITS were employed to identify the Antarctic isolates more precisely. Several other microalgae related to our isolates were obtained from international culture collections for comparison. The obtained results allowed the re-establishment of strain P. insigne SAG 30.93 as the reference strain and the definition of the molecular borders of both genera Pleurastrum and Chlorococcum. Based on our findings, several Chlorococcum species are now re-attributed to Pleurastrum, as well as Macrochloris rubrioleum, here re-named Pleurastrum rubrioleum comb. nov., to which the Antarctic isolates also belong. Finally, a relationship between Pleurastrum lineages and climate zones was established. Article in Journal/Newspaper Antarc* Antarctic Padua Research Archive (IRIS - Università degli Studi di Padova) Antarctic The Antarctic Diversity 15 5 650 |
institution |
Open Polar |
collection |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
op_collection_id |
ftunivpadovairis |
language |
English |
topic |
18S rRNA Chlorococcum climate zones ITS2 secondary structure Macrochloris rubrioleum Pleurastrum rbcL tufA |
spellingShingle |
18S rRNA Chlorococcum climate zones ITS2 secondary structure Macrochloris rubrioleum Pleurastrum rbcL tufA Sciuto K. Wolf M. A. Mistri M. Moro I. Appraisal of the Genus Pleurastrum (Chlorophyta) Based on Molecular and Climate Data |
topic_facet |
18S rRNA Chlorococcum climate zones ITS2 secondary structure Macrochloris rubrioleum Pleurastrum rbcL tufA |
description |
Two green microalgal strains, CCCryo 469-16 and CCCryo 470-16, were isolated from samples of Antarctic microflora. Their morphology and 18S rRNA sequences indicated a phylogenetic relationship with strains of the genera Chlorococcum Meneghini, Macrochloris Korshikov, and Pleurastrum Chodat. The last genus is considered particularly problematic as it underwent several revisions. Moreover, its type strain, P. insigne SAG 30.93, was recently demonstrated to coincide genetically with the authentic strain of Chlorococcum oleofaciens from an 18S rRNA phylogeny. This deprived the genus Pleurastrum of an important type reference. Thus, the molecular markers rbcL, tufA, and ITS were employed to identify the Antarctic isolates more precisely. Several other microalgae related to our isolates were obtained from international culture collections for comparison. The obtained results allowed the re-establishment of strain P. insigne SAG 30.93 as the reference strain and the definition of the molecular borders of both genera Pleurastrum and Chlorococcum. Based on our findings, several Chlorococcum species are now re-attributed to Pleurastrum, as well as Macrochloris rubrioleum, here re-named Pleurastrum rubrioleum comb. nov., to which the Antarctic isolates also belong. Finally, a relationship between Pleurastrum lineages and climate zones was established. |
author2 |
Sciuto, K. Wolf, M. A. Mistri, M. Moro, I. |
format |
Article in Journal/Newspaper |
author |
Sciuto K. Wolf M. A. Mistri M. Moro I. |
author_facet |
Sciuto K. Wolf M. A. Mistri M. Moro I. |
author_sort |
Sciuto K. |
title |
Appraisal of the Genus Pleurastrum (Chlorophyta) Based on Molecular and Climate Data |
title_short |
Appraisal of the Genus Pleurastrum (Chlorophyta) Based on Molecular and Climate Data |
title_full |
Appraisal of the Genus Pleurastrum (Chlorophyta) Based on Molecular and Climate Data |
title_fullStr |
Appraisal of the Genus Pleurastrum (Chlorophyta) Based on Molecular and Climate Data |
title_full_unstemmed |
Appraisal of the Genus Pleurastrum (Chlorophyta) Based on Molecular and Climate Data |
title_sort |
appraisal of the genus pleurastrum (chlorophyta) based on molecular and climate data |
publisher |
MDPIST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND |
publishDate |
2023 |
url |
https://hdl.handle.net/11577/3495947 https://doi.org/10.3390/d15050650 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000997228900001 volume:15 issue:5 firstpage:1 lastpage:1 numberofpages:1 journal:DIVERSITY https://hdl.handle.net/11577/3495947 doi:10.3390/d15050650 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85160678185 |
op_doi |
https://doi.org/10.3390/d15050650 |
container_title |
Diversity |
container_volume |
15 |
container_issue |
5 |
container_start_page |
650 |
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1796300814682161152 |