Biological activities of the aerial and undergound parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) from the Italian Alps
: This study investigated the bioactivity of both aerial (GNAR) and underground (GNUG) parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) (Orchidaceae). The obtained data proved interesting when the samples were tested in two adrenocortical cancer cell lines (SW13 and H295R). In...
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Online Access: | https://hdl.handle.net/11577/3495685 https://doi.org/10.1080/14786419.2023.2258437 |
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ftunivpadovairis:oai:www.research.unipd.it:11577/3495685 2024-04-14T08:15:11+00:00 Biological activities of the aerial and undergound parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) from the Italian Alps Vitalini, Sara Rubin, Beatrice Monticelli, Halenya Barollo, Susi Redaelli, Marco Bertazza, Loris Mian, Caterina Zorzan, Maira Garzoli, Stefania Iriti, Marcello Pezzani, Raffaele Vitalini, Sara Rubin, Beatrice Monticelli, Halenya Barollo, Susi Redaelli, Marco Bertazza, Lori Mian, Caterina Zorzan, Maira Garzoli, Stefania Iriti, Marcello Pezzani, Raffaele 2023 https://hdl.handle.net/11577/3495685 https://doi.org/10.1080/14786419.2023.2258437 eng eng info:eu-repo/semantics/altIdentifier/pmid/37732610 info:eu-repo/semantics/altIdentifier/wos/WOS:001071066300001 firstpage:1 lastpage:6 numberofpages:6 journal:NATURAL PRODUCT RESEARCH https://hdl.handle.net/11577/3495685 doi:10.1080/14786419.2023.2258437 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85171658104 Black vanilla orchid anti-inflammatory activity anti-proliferative activity antioxidant activity info:eu-repo/semantics/article 2023 ftunivpadovairis https://doi.org/10.1080/14786419.2023.2258437 2024-03-21T20:05:08Z : This study investigated the bioactivity of both aerial (GNAR) and underground (GNUG) parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) (Orchidaceae). The obtained data proved interesting when the samples were tested in two adrenocortical cancer cell lines (SW13 and H295R). In particular, the GNAR 80% methanol extract distinctly inhibited their viability after 24 h at a concentration of 1 μg/μL by MTT assay and trypan blue dye exclusion method. Cell morphology evaluation by means Wright's staining also showed significant results, particularly in SW13 cells under the effect of both extracts. GNAR extract was able to scavenge the DPPH radical better than GNUG extract. It also was more active in albumin denaturation (a maximum % denaturation equal to 463.0 ± 8.3 vs 77.3 ± 13.3) and protease inhibition (a maximum % inhibition equal to 138.5 ± 7.0 vs 2.1 ± 2.0) tests. The results highlighted an important antitumor activity of G. nigra in vitro that deserves to be further studied. Article in Journal/Newspaper Nigritella nigra Padua Research Archive (IRIS - Università degli Studi di Padova) Natural Product Research 1 6 |
institution |
Open Polar |
collection |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
op_collection_id |
ftunivpadovairis |
language |
English |
topic |
Black vanilla orchid anti-inflammatory activity anti-proliferative activity antioxidant activity |
spellingShingle |
Black vanilla orchid anti-inflammatory activity anti-proliferative activity antioxidant activity Vitalini, Sara Rubin, Beatrice Monticelli, Halenya Barollo, Susi Redaelli, Marco Bertazza, Loris Mian, Caterina Zorzan, Maira Garzoli, Stefania Iriti, Marcello Pezzani, Raffaele Biological activities of the aerial and undergound parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) from the Italian Alps |
topic_facet |
Black vanilla orchid anti-inflammatory activity anti-proliferative activity antioxidant activity |
description |
: This study investigated the bioactivity of both aerial (GNAR) and underground (GNUG) parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) (Orchidaceae). The obtained data proved interesting when the samples were tested in two adrenocortical cancer cell lines (SW13 and H295R). In particular, the GNAR 80% methanol extract distinctly inhibited their viability after 24 h at a concentration of 1 μg/μL by MTT assay and trypan blue dye exclusion method. Cell morphology evaluation by means Wright's staining also showed significant results, particularly in SW13 cells under the effect of both extracts. GNAR extract was able to scavenge the DPPH radical better than GNUG extract. It also was more active in albumin denaturation (a maximum % denaturation equal to 463.0 ± 8.3 vs 77.3 ± 13.3) and protease inhibition (a maximum % inhibition equal to 138.5 ± 7.0 vs 2.1 ± 2.0) tests. The results highlighted an important antitumor activity of G. nigra in vitro that deserves to be further studied. |
author2 |
Vitalini, Sara Rubin, Beatrice Monticelli, Halenya Barollo, Susi Redaelli, Marco Bertazza, Lori Mian, Caterina Zorzan, Maira Garzoli, Stefania Iriti, Marcello Pezzani, Raffaele |
format |
Article in Journal/Newspaper |
author |
Vitalini, Sara Rubin, Beatrice Monticelli, Halenya Barollo, Susi Redaelli, Marco Bertazza, Loris Mian, Caterina Zorzan, Maira Garzoli, Stefania Iriti, Marcello Pezzani, Raffaele |
author_facet |
Vitalini, Sara Rubin, Beatrice Monticelli, Halenya Barollo, Susi Redaelli, Marco Bertazza, Loris Mian, Caterina Zorzan, Maira Garzoli, Stefania Iriti, Marcello Pezzani, Raffaele |
author_sort |
Vitalini, Sara |
title |
Biological activities of the aerial and undergound parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) from the Italian Alps |
title_short |
Biological activities of the aerial and undergound parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) from the Italian Alps |
title_full |
Biological activities of the aerial and undergound parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) from the Italian Alps |
title_fullStr |
Biological activities of the aerial and undergound parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) from the Italian Alps |
title_full_unstemmed |
Biological activities of the aerial and undergound parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) from the Italian Alps |
title_sort |
biological activities of the aerial and undergound parts of gymnadenia nigra rchb.f. (syn. nigritella nigra (l.) rchb. f.) from the italian alps |
publishDate |
2023 |
url |
https://hdl.handle.net/11577/3495685 https://doi.org/10.1080/14786419.2023.2258437 |
genre |
Nigritella nigra |
genre_facet |
Nigritella nigra |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/37732610 info:eu-repo/semantics/altIdentifier/wos/WOS:001071066300001 firstpage:1 lastpage:6 numberofpages:6 journal:NATURAL PRODUCT RESEARCH https://hdl.handle.net/11577/3495685 doi:10.1080/14786419.2023.2258437 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85171658104 |
op_doi |
https://doi.org/10.1080/14786419.2023.2258437 |
container_title |
Natural Product Research |
container_start_page |
1 |
op_container_end_page |
6 |
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1796313460302151680 |