Microbiome structure in biofilms from a volcanic island in Maritime Antarctica investigated by genome-centric metagenomics and metatranscriptomics
Antarctica is the coldest and driest continent on Earth, characterized by polyextreme environmental conditions, where species adapted form complex networks of interactions. Microbial communities growing in these harsh environments can form biofilms that help the associated species to survive and thr...
Published in: | Microbiological Research |
---|---|
Main Authors: | , , , , |
Other Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier GmbH
2022
|
Subjects: | |
Online Access: | https://hdl.handle.net/11577/3471403 https://doi.org/10.1016/j.micres.2022.127197 https://www.sciencedirect.com/science/article/pii/S0944501322002373?via=ihub |
id |
ftunivpadovairis:oai:www.research.unipd.it:11577/3471403 |
---|---|
record_format |
openpolar |
spelling |
ftunivpadovairis:oai:www.research.unipd.it:11577/3471403 2024-02-27T08:34:45+00:00 Microbiome structure in biofilms from a volcanic island in Maritime Antarctica investigated by genome-centric metagenomics and metatranscriptomics Centurion V. B. Campanaro S. Basile A. Treu L. Oliveira V. M. Centurion, V. B. Campanaro, S. Basile, A. Treu, L. Oliveira, V. M. 2022 ELETTRONICO https://hdl.handle.net/11577/3471403 https://doi.org/10.1016/j.micres.2022.127197 https://www.sciencedirect.com/science/article/pii/S0944501322002373?via=ihub eng eng Elsevier GmbH info:eu-repo/semantics/altIdentifier/pmid/36174355 info:eu-repo/semantics/altIdentifier/wos/WOS:000863947900003 volume:265 firstpage:127197 journal:MICROBIOLOGICAL RESEARCH https://hdl.handle.net/11577/3471403 doi:10.1016/j.micres.2022.127197 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85138470508 https://www.sciencedirect.com/science/article/pii/S0944501322002373?via=ihub Correlation network Genome-centric metagenomic Purple bacteria Stress response Whalers Bay info:eu-repo/semantics/article 2022 ftunivpadovairis https://doi.org/10.1016/j.micres.2022.127197 2024-01-31T17:32:09Z Antarctica is the coldest and driest continent on Earth, characterized by polyextreme environmental conditions, where species adapted form complex networks of interactions. Microbial communities growing in these harsh environments can form biofilms that help the associated species to survive and thrive. A rich body of knowledge describes environmental biofilm communities; however, most studies have focused on dominant community members rather than functional complexity and metabolic potential. To overcome these limitations, the present study used genome-centric metagenomics to describe two biofilm samples subjected to different temperature collected in Deception Island, Maritime Antarctica. The results unraveled a complex biofilm microbiome represented by 180 metagenome-assembled genomes. The potential metabolic interactions were investigated using metabolic flux balance analysis and revealed that purple bacteria are the community members with the highest correlations with other bacteria. Due to their predicted mixotrophic behavior, they may play a crucial role in the microbiome, likely supporting the heterotrophic species in biofilms. Metatranscriptomics results revealed that the chaperone system and proteins counteracting ROS and toxic compounds have a major role in maintaining bacterial cell homeostasis in sediments of volcanic origin. Article in Journal/Newspaper Antarc* Antarctica Deception Island Padua Research Archive (IRIS - Università degli Studi di Padova) Deception Island ENVELOPE(-60.633,-60.633,-62.950,-62.950) Whalers Bay ENVELOPE(69.917,69.917,-49.100,-49.100) Microbiological Research 265 127197 |
institution |
Open Polar |
collection |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
op_collection_id |
ftunivpadovairis |
language |
English |
topic |
Correlation network Genome-centric metagenomic Purple bacteria Stress response Whalers Bay |
spellingShingle |
Correlation network Genome-centric metagenomic Purple bacteria Stress response Whalers Bay Centurion V. B. Campanaro S. Basile A. Treu L. Oliveira V. M. Microbiome structure in biofilms from a volcanic island in Maritime Antarctica investigated by genome-centric metagenomics and metatranscriptomics |
topic_facet |
Correlation network Genome-centric metagenomic Purple bacteria Stress response Whalers Bay |
description |
Antarctica is the coldest and driest continent on Earth, characterized by polyextreme environmental conditions, where species adapted form complex networks of interactions. Microbial communities growing in these harsh environments can form biofilms that help the associated species to survive and thrive. A rich body of knowledge describes environmental biofilm communities; however, most studies have focused on dominant community members rather than functional complexity and metabolic potential. To overcome these limitations, the present study used genome-centric metagenomics to describe two biofilm samples subjected to different temperature collected in Deception Island, Maritime Antarctica. The results unraveled a complex biofilm microbiome represented by 180 metagenome-assembled genomes. The potential metabolic interactions were investigated using metabolic flux balance analysis and revealed that purple bacteria are the community members with the highest correlations with other bacteria. Due to their predicted mixotrophic behavior, they may play a crucial role in the microbiome, likely supporting the heterotrophic species in biofilms. Metatranscriptomics results revealed that the chaperone system and proteins counteracting ROS and toxic compounds have a major role in maintaining bacterial cell homeostasis in sediments of volcanic origin. |
author2 |
Centurion, V. B. Campanaro, S. Basile, A. Treu, L. Oliveira, V. M. |
format |
Article in Journal/Newspaper |
author |
Centurion V. B. Campanaro S. Basile A. Treu L. Oliveira V. M. |
author_facet |
Centurion V. B. Campanaro S. Basile A. Treu L. Oliveira V. M. |
author_sort |
Centurion V. B. |
title |
Microbiome structure in biofilms from a volcanic island in Maritime Antarctica investigated by genome-centric metagenomics and metatranscriptomics |
title_short |
Microbiome structure in biofilms from a volcanic island in Maritime Antarctica investigated by genome-centric metagenomics and metatranscriptomics |
title_full |
Microbiome structure in biofilms from a volcanic island in Maritime Antarctica investigated by genome-centric metagenomics and metatranscriptomics |
title_fullStr |
Microbiome structure in biofilms from a volcanic island in Maritime Antarctica investigated by genome-centric metagenomics and metatranscriptomics |
title_full_unstemmed |
Microbiome structure in biofilms from a volcanic island in Maritime Antarctica investigated by genome-centric metagenomics and metatranscriptomics |
title_sort |
microbiome structure in biofilms from a volcanic island in maritime antarctica investigated by genome-centric metagenomics and metatranscriptomics |
publisher |
Elsevier GmbH |
publishDate |
2022 |
url |
https://hdl.handle.net/11577/3471403 https://doi.org/10.1016/j.micres.2022.127197 https://www.sciencedirect.com/science/article/pii/S0944501322002373?via=ihub |
long_lat |
ENVELOPE(-60.633,-60.633,-62.950,-62.950) ENVELOPE(69.917,69.917,-49.100,-49.100) |
geographic |
Deception Island Whalers Bay |
geographic_facet |
Deception Island Whalers Bay |
genre |
Antarc* Antarctica Deception Island |
genre_facet |
Antarc* Antarctica Deception Island |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/36174355 info:eu-repo/semantics/altIdentifier/wos/WOS:000863947900003 volume:265 firstpage:127197 journal:MICROBIOLOGICAL RESEARCH https://hdl.handle.net/11577/3471403 doi:10.1016/j.micres.2022.127197 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85138470508 https://www.sciencedirect.com/science/article/pii/S0944501322002373?via=ihub |
op_doi |
https://doi.org/10.1016/j.micres.2022.127197 |
container_title |
Microbiological Research |
container_volume |
265 |
container_start_page |
127197 |
_version_ |
1792056210096128000 |