Molecular Evolution of Metallothioneins of Antarctic Fish: A Physiological Adaptation to Peculiar Seawater Chemical Characteristics
Metallothioneins (MTs) are low-molecular weight sulfur-rich proteins, widely distributed in nature. They play a homeostatic role in the control and detoxification of metal ions. Previous studies indicated that MTs also have the capacity to scavenge reactive oxygen species. This study aimed to invest...
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Online Access: | https://hdl.handle.net/11577/3463061 https://doi.org/10.3390/jmse10111592 |
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ftunivpadovairis:oai:www.research.unipd.it:11577/3463061 2024-04-14T08:01:12+00:00 Molecular Evolution of Metallothioneins of Antarctic Fish: A Physiological Adaptation to Peculiar Seawater Chemical Characteristics Bakiu R. Boldrin F. Pacchini S. Schumann S. Piva E. Tolomeo A. M. Ferro D. Grapputo A. Santovito G. Irato P. Bakiu, R. Boldrin, F. Pacchini, S. Schumann, S. Piva, E. Tolomeo, A. M. Ferro, D. Grapputo, A. Santovito, G. Irato, P. 2022 STAMPA https://hdl.handle.net/11577/3463061 https://doi.org/10.3390/jmse10111592 eng eng MDPI info:eu-repo/semantics/altIdentifier/wos/WOS:000882240100001 volume:10 issue:11 firstpage:1592 journal:JOURNAL OF MARINE SCIENCE AND ENGINEERING https://hdl.handle.net/11577/3463061 doi:10.3390/jmse10111592 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85141843826 info:eu-repo/semantics/openAccess adaptation Antarctica fish metallothionein negative selection positive selection reticulate evolution info:eu-repo/semantics/article 2022 ftunivpadovairis https://doi.org/10.3390/jmse10111592 2024-03-21T18:40:54Z Metallothioneins (MTs) are low-molecular weight sulfur-rich proteins, widely distributed in nature. They play a homeostatic role in the control and detoxification of metal ions. Previous studies indicated that MTs also have the capacity to scavenge reactive oxygen species. This study aimed to investigate the evolution of the protein in the notothenioid fish, evolved under the selective pressure of relatively high oxygen partial pressures, characteristics of cold Antarctic seawaters, and relatively high concentrations of metal ions, Cd and Cu in particular. The cDNA sequences of MT isoforms were characterized in members of the Nototheniidae, Bathydraconidae, Artedidraconidae, and Channichthyidae families. The phylogenetic relationships of MTs from these families and other teleosts were inferred by using Maximum Likelihood and Bayesian methods. The analysis of coding region and untranslated (UTR) sequences indicated the presence of two MT clades, each containing one of the two MT isoforms, MT-1 and MT-2. Our results indicated, for the first time for these proteins, that the evolution of MT genes has been characterized by strong purifying selection, whereas it did not observe any evidence of positive selection. In addition, phylogenetic analysis of the UTRs suggested that functional changes, in particular related to the MT-1 gene expression, had accompanied the duplication event. Article in Journal/Newspaper Antarc* Antarctic Antarctica Padua Research Archive (IRIS - Università degli Studi di Padova) Antarctic Journal of Marine Science and Engineering 10 11 1592 |
institution |
Open Polar |
collection |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
op_collection_id |
ftunivpadovairis |
language |
English |
topic |
adaptation Antarctica fish metallothionein negative selection positive selection reticulate evolution |
spellingShingle |
adaptation Antarctica fish metallothionein negative selection positive selection reticulate evolution Bakiu R. Boldrin F. Pacchini S. Schumann S. Piva E. Tolomeo A. M. Ferro D. Grapputo A. Santovito G. Irato P. Molecular Evolution of Metallothioneins of Antarctic Fish: A Physiological Adaptation to Peculiar Seawater Chemical Characteristics |
topic_facet |
adaptation Antarctica fish metallothionein negative selection positive selection reticulate evolution |
description |
Metallothioneins (MTs) are low-molecular weight sulfur-rich proteins, widely distributed in nature. They play a homeostatic role in the control and detoxification of metal ions. Previous studies indicated that MTs also have the capacity to scavenge reactive oxygen species. This study aimed to investigate the evolution of the protein in the notothenioid fish, evolved under the selective pressure of relatively high oxygen partial pressures, characteristics of cold Antarctic seawaters, and relatively high concentrations of metal ions, Cd and Cu in particular. The cDNA sequences of MT isoforms were characterized in members of the Nototheniidae, Bathydraconidae, Artedidraconidae, and Channichthyidae families. The phylogenetic relationships of MTs from these families and other teleosts were inferred by using Maximum Likelihood and Bayesian methods. The analysis of coding region and untranslated (UTR) sequences indicated the presence of two MT clades, each containing one of the two MT isoforms, MT-1 and MT-2. Our results indicated, for the first time for these proteins, that the evolution of MT genes has been characterized by strong purifying selection, whereas it did not observe any evidence of positive selection. In addition, phylogenetic analysis of the UTRs suggested that functional changes, in particular related to the MT-1 gene expression, had accompanied the duplication event. |
author2 |
Bakiu, R. Boldrin, F. Pacchini, S. Schumann, S. Piva, E. Tolomeo, A. M. Ferro, D. Grapputo, A. Santovito, G. Irato, P. |
format |
Article in Journal/Newspaper |
author |
Bakiu R. Boldrin F. Pacchini S. Schumann S. Piva E. Tolomeo A. M. Ferro D. Grapputo A. Santovito G. Irato P. |
author_facet |
Bakiu R. Boldrin F. Pacchini S. Schumann S. Piva E. Tolomeo A. M. Ferro D. Grapputo A. Santovito G. Irato P. |
author_sort |
Bakiu R. |
title |
Molecular Evolution of Metallothioneins of Antarctic Fish: A Physiological Adaptation to Peculiar Seawater Chemical Characteristics |
title_short |
Molecular Evolution of Metallothioneins of Antarctic Fish: A Physiological Adaptation to Peculiar Seawater Chemical Characteristics |
title_full |
Molecular Evolution of Metallothioneins of Antarctic Fish: A Physiological Adaptation to Peculiar Seawater Chemical Characteristics |
title_fullStr |
Molecular Evolution of Metallothioneins of Antarctic Fish: A Physiological Adaptation to Peculiar Seawater Chemical Characteristics |
title_full_unstemmed |
Molecular Evolution of Metallothioneins of Antarctic Fish: A Physiological Adaptation to Peculiar Seawater Chemical Characteristics |
title_sort |
molecular evolution of metallothioneins of antarctic fish: a physiological adaptation to peculiar seawater chemical characteristics |
publisher |
MDPI |
publishDate |
2022 |
url |
https://hdl.handle.net/11577/3463061 https://doi.org/10.3390/jmse10111592 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000882240100001 volume:10 issue:11 firstpage:1592 journal:JOURNAL OF MARINE SCIENCE AND ENGINEERING https://hdl.handle.net/11577/3463061 doi:10.3390/jmse10111592 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85141843826 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/jmse10111592 |
container_title |
Journal of Marine Science and Engineering |
container_volume |
10 |
container_issue |
11 |
container_start_page |
1592 |
_version_ |
1796307934072799232 |