ADAR-editing during Ostreid herpesvirus 1 infection in Crassostrea gigas: facts and limitations
Ostreid herpesvirus-1 (OsHV-1) RNAs are enzymatically modified by A-to-I conversions during the infection of Crassostrea gigas. The increase of ADAR1 expression and hyper-editing activity parallel to OsHV-1 RNAs suggests a functional connection between dsRNA editing and antiviral responses. We analy...
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AMER SOC MICROBIOLOGY
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ftunivpadovairis:oai:www.research.unipd.it:11577/3420297 2024-02-27T08:39:52+00:00 ADAR-editing during Ostreid herpesvirus 1 infection in Crassostrea gigas: facts and limitations Umberto Rosani Enrico Bortoletto Caroline Montagnani Paola Venier Rosani, Umberto Bortoletto, Enrico Montagnani, Caroline Venier, Paola 2022 http://hdl.handle.net/11577/3420297 https://doi.org/10.1128/msphere.00011-22 eng eng AMER SOC MICROBIOLOGY info:eu-repo/semantics/altIdentifier/pmid/35379005 info:eu-repo/semantics/altIdentifier/wos/WOS:000778021300001 volume:7 issue:2 firstpage:1 lastpage:8 numberofpages:8 journal:MSPHERE http://hdl.handle.net/11577/3420297 doi:10.1128/msphere.00011-22 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85129779307 ADAR1 hyper-editing oyster OsHV-1 antiviral immunity info:eu-repo/semantics/article 2022 ftunivpadovairis https://doi.org/10.1128/msphere.00011-22 2024-01-31T18:04:31Z Ostreid herpesvirus-1 (OsHV-1) RNAs are enzymatically modified by A-to-I conversions during the infection of Crassostrea gigas. The increase of ADAR1 expression and hyper-editing activity parallel to OsHV-1 RNAs suggests a functional connection between dsRNA editing and antiviral responses. We analyzed 87 RNA-seq datasets from immuno-primed, resistant, and susceptible oysters exposed to OsHV-1 to compare the ADAR hyper-editing levels on host and viral transcripts and trace hyper-editing on the oyster genes. Host RNAs were more hyper-edited than viral RNAs, despite the increased editing of viral RNAs in late infection phases. A set of genes, representing ~0.5% of the oyster transcriptome and including several Tripartite motif-containing sequences, were constantly hyper-edited. Conversely, we identified genes involved in antiviral response, miRNA maturation and epigenetic regulation which were hyper-edited in specific conditions only. Despite technical and biological bottlenecks that hamper the understanding of the bivalve ‘RNA editome’, available tools and technologies can be adapted to bivalve mollusks. Article in Journal/Newspaper Crassostrea gigas Padua Research Archive (IRIS - Università degli Studi di Padova) mSphere |
institution |
Open Polar |
collection |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
op_collection_id |
ftunivpadovairis |
language |
English |
topic |
ADAR1 hyper-editing oyster OsHV-1 antiviral immunity |
spellingShingle |
ADAR1 hyper-editing oyster OsHV-1 antiviral immunity Umberto Rosani Enrico Bortoletto Caroline Montagnani Paola Venier ADAR-editing during Ostreid herpesvirus 1 infection in Crassostrea gigas: facts and limitations |
topic_facet |
ADAR1 hyper-editing oyster OsHV-1 antiviral immunity |
description |
Ostreid herpesvirus-1 (OsHV-1) RNAs are enzymatically modified by A-to-I conversions during the infection of Crassostrea gigas. The increase of ADAR1 expression and hyper-editing activity parallel to OsHV-1 RNAs suggests a functional connection between dsRNA editing and antiviral responses. We analyzed 87 RNA-seq datasets from immuno-primed, resistant, and susceptible oysters exposed to OsHV-1 to compare the ADAR hyper-editing levels on host and viral transcripts and trace hyper-editing on the oyster genes. Host RNAs were more hyper-edited than viral RNAs, despite the increased editing of viral RNAs in late infection phases. A set of genes, representing ~0.5% of the oyster transcriptome and including several Tripartite motif-containing sequences, were constantly hyper-edited. Conversely, we identified genes involved in antiviral response, miRNA maturation and epigenetic regulation which were hyper-edited in specific conditions only. Despite technical and biological bottlenecks that hamper the understanding of the bivalve ‘RNA editome’, available tools and technologies can be adapted to bivalve mollusks. |
author2 |
Rosani, Umberto Bortoletto, Enrico Montagnani, Caroline Venier, Paola |
format |
Article in Journal/Newspaper |
author |
Umberto Rosani Enrico Bortoletto Caroline Montagnani Paola Venier |
author_facet |
Umberto Rosani Enrico Bortoletto Caroline Montagnani Paola Venier |
author_sort |
Umberto Rosani |
title |
ADAR-editing during Ostreid herpesvirus 1 infection in Crassostrea gigas: facts and limitations |
title_short |
ADAR-editing during Ostreid herpesvirus 1 infection in Crassostrea gigas: facts and limitations |
title_full |
ADAR-editing during Ostreid herpesvirus 1 infection in Crassostrea gigas: facts and limitations |
title_fullStr |
ADAR-editing during Ostreid herpesvirus 1 infection in Crassostrea gigas: facts and limitations |
title_full_unstemmed |
ADAR-editing during Ostreid herpesvirus 1 infection in Crassostrea gigas: facts and limitations |
title_sort |
adar-editing during ostreid herpesvirus 1 infection in crassostrea gigas: facts and limitations |
publisher |
AMER SOC MICROBIOLOGY |
publishDate |
2022 |
url |
http://hdl.handle.net/11577/3420297 https://doi.org/10.1128/msphere.00011-22 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/35379005 info:eu-repo/semantics/altIdentifier/wos/WOS:000778021300001 volume:7 issue:2 firstpage:1 lastpage:8 numberofpages:8 journal:MSPHERE http://hdl.handle.net/11577/3420297 doi:10.1128/msphere.00011-22 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85129779307 |
op_doi |
https://doi.org/10.1128/msphere.00011-22 |
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mSphere |
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