Severe gastroenteropathy associated with Clostridium perfringens isolation in starving juvenile sturgeons
In November 2020 a mortality episode (30%) in juvenile Siberian and Russian sturgeons (Acipenser baerii, Brandt, and A. gueldenstaedtii, Brandt & Ratzeburg) and GUBA hybrid sturgeons (A. gueldenstaedtii × A. baerii) occurred in a hatchery in Northern Italy, associated with severe coelomic disten...
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2022
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Online Access: | http://hdl.handle.net/11577/3413332 https://doi.org/10.1111/jfd.13579 |
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ftunivpadovairis:oai:www.research.unipd.it:11577/3413332 2024-04-21T07:43:29+00:00 Severe gastroenteropathy associated with Clostridium perfringens isolation in starving juvenile sturgeons Brocca G. Zamparo S. Pretto T. Calore A. Marsella A. Xiccato R. L. Cornaggia M. Cortinovis L. Bano L. Toffan A. Quaglio F. Verin R. Brocca, G. Zamparo, S. Pretto, T. Calore, A. Marsella, A. Xiccato, R. L. Cornaggia, M. Cortinovis, L. Bano, L. Toffan, A. Quaglio, F. Verin, R. 2022 http://hdl.handle.net/11577/3413332 https://doi.org/10.1111/jfd.13579 eng eng John Wiley and Sons Inc info:eu-repo/semantics/altIdentifier/pmid/35007367 info:eu-repo/semantics/altIdentifier/wos/WOS:000740795800001 journal:JOURNAL OF FISH DISEASES http://hdl.handle.net/11577/3413332 doi:10.1111/jfd.13579 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85122681369 C perfringen dysmicrobism gastroenteropathy starvation sturgeon toxin info:eu-repo/semantics/article 2022 ftunivpadovairis https://doi.org/10.1111/jfd.13579 2024-03-28T02:11:04Z In November 2020 a mortality episode (30%) in juvenile Siberian and Russian sturgeons (Acipenser baerii, Brandt, and A. gueldenstaedtii, Brandt & Ratzeburg) and GUBA hybrid sturgeons (A. gueldenstaedtii × A. baerii) occurred in a hatchery in Northern Italy, associated with severe coelomic distension and abnormal reverse surface swimming. The fish were reared in concrete tanks supplied by well water, fed at 0.4% of body weight (b.w.) per day. Thirty sturgeon specimens were collected for necropsy, histological, bacteriological and virological examination. Macroscopic findings included diffuse and severe bloating of gastrointestinal tracts due to foamy contents with thinning and stretching of the gastrointestinal walls. Histological analysis revealed variable degrees of sloughing and necrosis of the intestinal epithelium, and the presence of bacterial aggregates. Anaerobic Gram-positive bacteria were investigated, and Clostridium perfringens was isolated from the gut. Specific PCRs identified the toxinotype A and the β2 toxin gene. The daily feed administration was increased to 1.5% b.w. and after 5 days, the mortality ceased. A new animal cohort from the same groups was examined after 12 weeks, showing neither gut alterations nor isolation of C. perfringens. The imbalance of intestinal microbiota, presumably caused by underfeeding, favoured C. perfringens overgrowth and severe gas formation. The diet increase possibly restored the normal microbiota. Article in Journal/Newspaper Acipenser baerii Padua Research Archive (IRIS - Università degli Studi di Padova) Journal of Fish Diseases |
institution |
Open Polar |
collection |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
op_collection_id |
ftunivpadovairis |
language |
English |
topic |
C perfringen dysmicrobism gastroenteropathy starvation sturgeon toxin |
spellingShingle |
C perfringen dysmicrobism gastroenteropathy starvation sturgeon toxin Brocca G. Zamparo S. Pretto T. Calore A. Marsella A. Xiccato R. L. Cornaggia M. Cortinovis L. Bano L. Toffan A. Quaglio F. Verin R. Severe gastroenteropathy associated with Clostridium perfringens isolation in starving juvenile sturgeons |
topic_facet |
C perfringen dysmicrobism gastroenteropathy starvation sturgeon toxin |
description |
In November 2020 a mortality episode (30%) in juvenile Siberian and Russian sturgeons (Acipenser baerii, Brandt, and A. gueldenstaedtii, Brandt & Ratzeburg) and GUBA hybrid sturgeons (A. gueldenstaedtii × A. baerii) occurred in a hatchery in Northern Italy, associated with severe coelomic distension and abnormal reverse surface swimming. The fish were reared in concrete tanks supplied by well water, fed at 0.4% of body weight (b.w.) per day. Thirty sturgeon specimens were collected for necropsy, histological, bacteriological and virological examination. Macroscopic findings included diffuse and severe bloating of gastrointestinal tracts due to foamy contents with thinning and stretching of the gastrointestinal walls. Histological analysis revealed variable degrees of sloughing and necrosis of the intestinal epithelium, and the presence of bacterial aggregates. Anaerobic Gram-positive bacteria were investigated, and Clostridium perfringens was isolated from the gut. Specific PCRs identified the toxinotype A and the β2 toxin gene. The daily feed administration was increased to 1.5% b.w. and after 5 days, the mortality ceased. A new animal cohort from the same groups was examined after 12 weeks, showing neither gut alterations nor isolation of C. perfringens. The imbalance of intestinal microbiota, presumably caused by underfeeding, favoured C. perfringens overgrowth and severe gas formation. The diet increase possibly restored the normal microbiota. |
author2 |
Brocca, G. Zamparo, S. Pretto, T. Calore, A. Marsella, A. Xiccato, R. L. Cornaggia, M. Cortinovis, L. Bano, L. Toffan, A. Quaglio, F. Verin, R. |
format |
Article in Journal/Newspaper |
author |
Brocca G. Zamparo S. Pretto T. Calore A. Marsella A. Xiccato R. L. Cornaggia M. Cortinovis L. Bano L. Toffan A. Quaglio F. Verin R. |
author_facet |
Brocca G. Zamparo S. Pretto T. Calore A. Marsella A. Xiccato R. L. Cornaggia M. Cortinovis L. Bano L. Toffan A. Quaglio F. Verin R. |
author_sort |
Brocca G. |
title |
Severe gastroenteropathy associated with Clostridium perfringens isolation in starving juvenile sturgeons |
title_short |
Severe gastroenteropathy associated with Clostridium perfringens isolation in starving juvenile sturgeons |
title_full |
Severe gastroenteropathy associated with Clostridium perfringens isolation in starving juvenile sturgeons |
title_fullStr |
Severe gastroenteropathy associated with Clostridium perfringens isolation in starving juvenile sturgeons |
title_full_unstemmed |
Severe gastroenteropathy associated with Clostridium perfringens isolation in starving juvenile sturgeons |
title_sort |
severe gastroenteropathy associated with clostridium perfringens isolation in starving juvenile sturgeons |
publisher |
John Wiley and Sons Inc |
publishDate |
2022 |
url |
http://hdl.handle.net/11577/3413332 https://doi.org/10.1111/jfd.13579 |
genre |
Acipenser baerii |
genre_facet |
Acipenser baerii |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/35007367 info:eu-repo/semantics/altIdentifier/wos/WOS:000740795800001 journal:JOURNAL OF FISH DISEASES http://hdl.handle.net/11577/3413332 doi:10.1111/jfd.13579 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85122681369 |
op_doi |
https://doi.org/10.1111/jfd.13579 |
container_title |
Journal of Fish Diseases |
_version_ |
1796946998435250176 |