Characterising the potential of sheep wool for ancient DNA analyses
The use of wool derived from sheep (Ovis aries) hair shafts is widespread in ancient and historic textiles. Given that hair can represent a valuable source of ancient DNA, wool may represent a valuable genetic archive for studies on the domestication of the sheep. However, both the quality and conte...
Published in: | Archaeological and Anthropological Sciences |
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Online Access: | http://hdl.handle.net/11577/3405152 https://doi.org/10.1007/s12520-011-0055-2 |
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ftunivpadovairis:oai:www.research.unipd.it:11577/3405152 2024-04-14T08:12:38+00:00 Characterising the potential of sheep wool for ancient DNA analyses Luise Ørsted Brandt Lena Diana Tranekjer Ulla Mannering Maj Ringgaard Karin Margarita Frei Eske Willerslev Margarita Gleba M. Thomas P. Gilbert Ørsted Brandt, Luise Diana Tranekjer, Lena Mannering, Ulla Ringgaard, Maj Margarita Frei, Karin Willerslev, Eske Gleba, Margarita Gilbert, M. Thomas P. 2011 STAMPA http://hdl.handle.net/11577/3405152 https://doi.org/10.1007/s12520-011-0055-2 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000208772500006 volume:3 issue:2 firstpage:209 lastpage:221 numberofpages:13 journal:ARCHAEOLOGICAL AND ANTHROPOLOGICAL SCIENCES http://hdl.handle.net/11577/3405152 doi:10.1007/s12520-011-0055-2 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84855516444 Ancient DNA Mitochondria Nuclear Sheep Textile Wool info:eu-repo/semantics/article 2011 ftunivpadovairis https://doi.org/10.1007/s12520-011-0055-2 2024-03-21T19:48:58Z The use of wool derived from sheep (Ovis aries) hair shafts is widespread in ancient and historic textiles. Given that hair can represent a valuable source of ancient DNA, wool may represent a valuable genetic archive for studies on the domestication of the sheep. However, both the quality and content of DNA in hair shafts are known to vary, and it is possible that common treatments of wool such as dyeing may negatively impact the DNA. Using quantitative real-time polymerase chain reaction (PCR), we demonstrate that in general, short fragments of both mitochondrial and single-copy nuclear DNA can be PCR-amplified from wool derived from a variety of breeds, regardless of the body location or natural pigmentation. Furthermore, although DNA can be PCR-amplified from wool dyed with one of four common plant dyes (tansy, woad, madder, weld), the use of mordants such as alum or iron leads to considerable DNA degradation. Lastly, we demonstrate that mtDNA at least can be PCR-amplified, cloned and sequenced from a range of archaeological and historic Danish, Flemmish and Greenlandic wool textile samples. In summary, our data suggest that wool offers a promising source for future ancient mitochondrial DNA studies. Article in Journal/Newspaper greenlandic Padua Research Archive (IRIS - Università degli Studi di Padova) Madder ENVELOPE(-56.483,-56.483,-63.300,-63.300) Archaeological and Anthropological Sciences 3 2 209 221 |
institution |
Open Polar |
collection |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
op_collection_id |
ftunivpadovairis |
language |
English |
topic |
Ancient DNA Mitochondria Nuclear Sheep Textile Wool |
spellingShingle |
Ancient DNA Mitochondria Nuclear Sheep Textile Wool Luise Ørsted Brandt Lena Diana Tranekjer Ulla Mannering Maj Ringgaard Karin Margarita Frei Eske Willerslev Margarita Gleba M. Thomas P. Gilbert Characterising the potential of sheep wool for ancient DNA analyses |
topic_facet |
Ancient DNA Mitochondria Nuclear Sheep Textile Wool |
description |
The use of wool derived from sheep (Ovis aries) hair shafts is widespread in ancient and historic textiles. Given that hair can represent a valuable source of ancient DNA, wool may represent a valuable genetic archive for studies on the domestication of the sheep. However, both the quality and content of DNA in hair shafts are known to vary, and it is possible that common treatments of wool such as dyeing may negatively impact the DNA. Using quantitative real-time polymerase chain reaction (PCR), we demonstrate that in general, short fragments of both mitochondrial and single-copy nuclear DNA can be PCR-amplified from wool derived from a variety of breeds, regardless of the body location or natural pigmentation. Furthermore, although DNA can be PCR-amplified from wool dyed with one of four common plant dyes (tansy, woad, madder, weld), the use of mordants such as alum or iron leads to considerable DNA degradation. Lastly, we demonstrate that mtDNA at least can be PCR-amplified, cloned and sequenced from a range of archaeological and historic Danish, Flemmish and Greenlandic wool textile samples. In summary, our data suggest that wool offers a promising source for future ancient mitochondrial DNA studies. |
author2 |
Ørsted Brandt, Luise Diana Tranekjer, Lena Mannering, Ulla Ringgaard, Maj Margarita Frei, Karin Willerslev, Eske Gleba, Margarita Gilbert, M. Thomas P. |
format |
Article in Journal/Newspaper |
author |
Luise Ørsted Brandt Lena Diana Tranekjer Ulla Mannering Maj Ringgaard Karin Margarita Frei Eske Willerslev Margarita Gleba M. Thomas P. Gilbert |
author_facet |
Luise Ørsted Brandt Lena Diana Tranekjer Ulla Mannering Maj Ringgaard Karin Margarita Frei Eske Willerslev Margarita Gleba M. Thomas P. Gilbert |
author_sort |
Luise Ørsted Brandt |
title |
Characterising the potential of sheep wool for ancient DNA analyses |
title_short |
Characterising the potential of sheep wool for ancient DNA analyses |
title_full |
Characterising the potential of sheep wool for ancient DNA analyses |
title_fullStr |
Characterising the potential of sheep wool for ancient DNA analyses |
title_full_unstemmed |
Characterising the potential of sheep wool for ancient DNA analyses |
title_sort |
characterising the potential of sheep wool for ancient dna analyses |
publishDate |
2011 |
url |
http://hdl.handle.net/11577/3405152 https://doi.org/10.1007/s12520-011-0055-2 |
long_lat |
ENVELOPE(-56.483,-56.483,-63.300,-63.300) |
geographic |
Madder |
geographic_facet |
Madder |
genre |
greenlandic |
genre_facet |
greenlandic |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000208772500006 volume:3 issue:2 firstpage:209 lastpage:221 numberofpages:13 journal:ARCHAEOLOGICAL AND ANTHROPOLOGICAL SCIENCES http://hdl.handle.net/11577/3405152 doi:10.1007/s12520-011-0055-2 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84855516444 |
op_doi |
https://doi.org/10.1007/s12520-011-0055-2 |
container_title |
Archaeological and Anthropological Sciences |
container_volume |
3 |
container_issue |
2 |
container_start_page |
209 |
op_container_end_page |
221 |
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