Prokaryotic Response to Phytodetritus-Derived Organic Material in Epi- and Mesopelagic Antarctic Waters
Particulate organic matter (POM) export represents the underlying principle of the biological carbon pump, driving the carbon flux from the sunlit to the dark ocean. The efficiency of this process is tightly linked to the prokaryotic community, as >70% of POM respiration is carried out by particl...
Published in: | Frontiers in Microbiology |
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Online Access: | http://hdl.handle.net/11577/3355418 https://doi.org/10.3389/fmicb.2020.01242 |
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ftunivpadovairis:oai:www.research.unipd.it:11577/3355418 2024-04-21T07:51:41+00:00 Prokaryotic Response to Phytodetritus-Derived Organic Material in Epi- and Mesopelagic Antarctic Waters Manna V. Malfatti F. Banchi E. Cerino F. De Pascale F. Franzo A. Schiavon R. Vezzi A. Del Negro P. Celussi M. Manna, V. Malfatti, F. Banchi, E. Cerino, F. De Pascale, F. Franzo, A. Schiavon, R. Vezzi, A. Del Negro, P. Celussi, M. 2020 ELETTRONICO http://hdl.handle.net/11577/3355418 https://doi.org/10.3389/fmicb.2020.01242 eng eng Frontiers Media S.A. info:eu-repo/semantics/altIdentifier/pmid/32582131 info:eu-repo/semantics/altIdentifier/wos/WOS:000543513400001 volume:11 numberofpages:18 journal:FRONTIERS IN MICROBIOLOGY http://hdl.handle.net/11577/3355418 doi:10.3389/fmicb.2020.01242 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85087016941 info:eu-repo/semantics/openAccess 16S rRNA carbon cycle extracellular enzyme free-living microbial community particle-attached particulate organic matter Southern Ocean info:eu-repo/semantics/article 2020 ftunivpadovairis https://doi.org/10.3389/fmicb.2020.01242 2024-03-28T02:09:24Z Particulate organic matter (POM) export represents the underlying principle of the biological carbon pump, driving the carbon flux from the sunlit to the dark ocean. The efficiency of this process is tightly linked to the prokaryotic community, as >70% of POM respiration is carried out by particle-associated prokaryotes. In the Ross Sea, one of the most productive areas of the Southern Ocean, up to 50% of the surface primary production is exported to the mesopelagic ocean as POM. Recent evidence suggests that a significant fraction of the POM in this area is composed of intact phytoplankton cells. During austral summer 2017, we set up bottle enrichment experiments in which we amended free-living surface and deep prokaryotic communities with organic matter pools generated from native microplankton, mimicking the particle export that may derive from mild (1 μg of Chlorophyll a L–1) and intense (10 μg of Chlorophyll a L–1) phytoplankton bloom. Over a course of 4 days, we followed free-living and particle-attached prokaryotes’ abundance, the degradation rates of polysaccharides, proteins and lipids, heterotrophic production as well as inorganic carbon utilization and prokaryotic community structure dynamics. Our results showed that several rare or undetected taxa in the initial community became dominant during the time course of the incubations and that different phytodetritus-derived organic matter sources induced specific changes in microbial communities, selecting for peculiar degradation and utilization processes spectra. Moreover, the features of the supplied detritus (in terms of microplankton taxa composition) determined different colonization dynamics and organic matter processing modes. Our study provides insights into the mechanisms underlying the prokaryotic utilization of phytodetritus, a significant pool of organic matter in the dark ocean. Article in Journal/Newspaper Antarc* Antarctic Ross Sea Southern Ocean Padua Research Archive (IRIS - Università degli Studi di Padova) Frontiers in Microbiology 11 |
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Open Polar |
collection |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
op_collection_id |
ftunivpadovairis |
language |
English |
topic |
16S rRNA carbon cycle extracellular enzyme free-living microbial community particle-attached particulate organic matter Southern Ocean |
spellingShingle |
16S rRNA carbon cycle extracellular enzyme free-living microbial community particle-attached particulate organic matter Southern Ocean Manna V. Malfatti F. Banchi E. Cerino F. De Pascale F. Franzo A. Schiavon R. Vezzi A. Del Negro P. Celussi M. Prokaryotic Response to Phytodetritus-Derived Organic Material in Epi- and Mesopelagic Antarctic Waters |
topic_facet |
16S rRNA carbon cycle extracellular enzyme free-living microbial community particle-attached particulate organic matter Southern Ocean |
description |
Particulate organic matter (POM) export represents the underlying principle of the biological carbon pump, driving the carbon flux from the sunlit to the dark ocean. The efficiency of this process is tightly linked to the prokaryotic community, as >70% of POM respiration is carried out by particle-associated prokaryotes. In the Ross Sea, one of the most productive areas of the Southern Ocean, up to 50% of the surface primary production is exported to the mesopelagic ocean as POM. Recent evidence suggests that a significant fraction of the POM in this area is composed of intact phytoplankton cells. During austral summer 2017, we set up bottle enrichment experiments in which we amended free-living surface and deep prokaryotic communities with organic matter pools generated from native microplankton, mimicking the particle export that may derive from mild (1 μg of Chlorophyll a L–1) and intense (10 μg of Chlorophyll a L–1) phytoplankton bloom. Over a course of 4 days, we followed free-living and particle-attached prokaryotes’ abundance, the degradation rates of polysaccharides, proteins and lipids, heterotrophic production as well as inorganic carbon utilization and prokaryotic community structure dynamics. Our results showed that several rare or undetected taxa in the initial community became dominant during the time course of the incubations and that different phytodetritus-derived organic matter sources induced specific changes in microbial communities, selecting for peculiar degradation and utilization processes spectra. Moreover, the features of the supplied detritus (in terms of microplankton taxa composition) determined different colonization dynamics and organic matter processing modes. Our study provides insights into the mechanisms underlying the prokaryotic utilization of phytodetritus, a significant pool of organic matter in the dark ocean. |
author2 |
Manna, V. Malfatti, F. Banchi, E. Cerino, F. De Pascale, F. Franzo, A. Schiavon, R. Vezzi, A. Del Negro, P. Celussi, M. |
format |
Article in Journal/Newspaper |
author |
Manna V. Malfatti F. Banchi E. Cerino F. De Pascale F. Franzo A. Schiavon R. Vezzi A. Del Negro P. Celussi M. |
author_facet |
Manna V. Malfatti F. Banchi E. Cerino F. De Pascale F. Franzo A. Schiavon R. Vezzi A. Del Negro P. Celussi M. |
author_sort |
Manna V. |
title |
Prokaryotic Response to Phytodetritus-Derived Organic Material in Epi- and Mesopelagic Antarctic Waters |
title_short |
Prokaryotic Response to Phytodetritus-Derived Organic Material in Epi- and Mesopelagic Antarctic Waters |
title_full |
Prokaryotic Response to Phytodetritus-Derived Organic Material in Epi- and Mesopelagic Antarctic Waters |
title_fullStr |
Prokaryotic Response to Phytodetritus-Derived Organic Material in Epi- and Mesopelagic Antarctic Waters |
title_full_unstemmed |
Prokaryotic Response to Phytodetritus-Derived Organic Material in Epi- and Mesopelagic Antarctic Waters |
title_sort |
prokaryotic response to phytodetritus-derived organic material in epi- and mesopelagic antarctic waters |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
http://hdl.handle.net/11577/3355418 https://doi.org/10.3389/fmicb.2020.01242 |
genre |
Antarc* Antarctic Ross Sea Southern Ocean |
genre_facet |
Antarc* Antarctic Ross Sea Southern Ocean |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/32582131 info:eu-repo/semantics/altIdentifier/wos/WOS:000543513400001 volume:11 numberofpages:18 journal:FRONTIERS IN MICROBIOLOGY http://hdl.handle.net/11577/3355418 doi:10.3389/fmicb.2020.01242 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85087016941 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3389/fmicb.2020.01242 |
container_title |
Frontiers in Microbiology |
container_volume |
11 |
_version_ |
1796935002037944320 |