Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1
Background: Since 2008, the aquaculture production of Crassostrea gigas was heavily affected by mass mortalities associated to Ostreid herpesvirus 1 (OsHV-1) microvariants worldwide. Transcriptomic studies revealed the major antiviral pathways of the oyster immune response while other findings sugge...
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BioMed Central (BMC) Part of Springer Nature
2020
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Online Access: | http://hdl.handle.net/11577/3350559 https://doi.org/10.1186/s12864-020-07026-7 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-07026-7 |
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ftunivpadovairis:oai:www.research.unipd.it:11577/3350559 2024-04-21T08:00:38+00:00 Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 Rosani Umberto Abbadi Miriam Green Timothy Bai Chang-Ming Turolla Edoardo Arcangeli Giuseppe Wegner K. Mathias Venier Paola Rosani, Umberto Abbadi, Miriam Green, Timothy Bai, Chang-Ming Turolla, Edoardo Arcangeli, Giuseppe Wegner K., Mathia Venier, Paola 2020 STAMPA http://hdl.handle.net/11577/3350559 https://doi.org/10.1186/s12864-020-07026-7 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-07026-7 eng eng BioMed Central (BMC) Part of Springer Nature info:eu-repo/semantics/altIdentifier/pmid/32912133 info:eu-repo/semantics/altIdentifier/wos/WOS:000571763500002 volume:21 firstpage:1 lastpage:18 numberofpages:18 journal:BMC GENOMICS info:eu-repo/grantAgreement/EC/H2020/678589 http://hdl.handle.net/11577/3350559 doi:10.1186/s12864-020-07026-7 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85090818382 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-07026-7 oyster miRNA OsHV-1 ADAR C. gigas miRNAome info:eu-repo/semantics/article 2020 ftunivpadovairis https://doi.org/10.1186/s12864-020-07026-7 2024-03-28T02:08:36Z Background: Since 2008, the aquaculture production of Crassostrea gigas was heavily affected by mass mortalities associated to Ostreid herpesvirus 1 (OsHV-1) microvariants worldwide. Transcriptomic studies revealed the major antiviral pathways of the oyster immune response while other findings suggested that also small non-coding RNAs (sncRNA) such as microRNAs might act as key regulators of the oyster response against OsHV-1. To explore the explicit connection between small non-coding and protein-coding transcripts, we performed paired whole transcriptome analysis of sncRNA and messenger RNA (mRNA) in six oysters selected for different intensities of OsHV-1 infection. Results: The mRNA profiles of the naturally infected oysters were mostly governed by the transcriptional activity of OsHV-1, with several differentially expressed genes mapping to the interferon, toll, apoptosis, and pro-PO pathways. In contrast, miRNA profiles suggested more complex regulatory mechanisms, with 15 differentially expressed miRNAs (DE-miRNA) pointing to a possible modulation of the host response during OsHV-1 infection. We predicted 68 interactions between DE-miRNAs and oyster 3′-UTRs, but only few of them involved antiviral genes. The sncRNA reads assigned to OsHV-1 rather resembled mRNA degradation products, suggesting the absence of genuine viral miRNAs. Conclusions: We provided data describing the miRNAome during OsHV-1 infection in C. gigas. This information can be used to understand the role of miRNAs in healthy and diseased oysters, to identify new targets for functional studies and, eventually to disentangle cause and effect relationships during viral infections in marine mollusks. Article in Journal/Newspaper Crassostrea gigas Padua Research Archive (IRIS - Università degli Studi di Padova) BMC Genomics 21 1 |
institution |
Open Polar |
collection |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
op_collection_id |
ftunivpadovairis |
language |
English |
topic |
oyster miRNA OsHV-1 ADAR C. gigas miRNAome |
spellingShingle |
oyster miRNA OsHV-1 ADAR C. gigas miRNAome Rosani Umberto Abbadi Miriam Green Timothy Bai Chang-Ming Turolla Edoardo Arcangeli Giuseppe Wegner K. Mathias Venier Paola Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 |
topic_facet |
oyster miRNA OsHV-1 ADAR C. gigas miRNAome |
description |
Background: Since 2008, the aquaculture production of Crassostrea gigas was heavily affected by mass mortalities associated to Ostreid herpesvirus 1 (OsHV-1) microvariants worldwide. Transcriptomic studies revealed the major antiviral pathways of the oyster immune response while other findings suggested that also small non-coding RNAs (sncRNA) such as microRNAs might act as key regulators of the oyster response against OsHV-1. To explore the explicit connection between small non-coding and protein-coding transcripts, we performed paired whole transcriptome analysis of sncRNA and messenger RNA (mRNA) in six oysters selected for different intensities of OsHV-1 infection. Results: The mRNA profiles of the naturally infected oysters were mostly governed by the transcriptional activity of OsHV-1, with several differentially expressed genes mapping to the interferon, toll, apoptosis, and pro-PO pathways. In contrast, miRNA profiles suggested more complex regulatory mechanisms, with 15 differentially expressed miRNAs (DE-miRNA) pointing to a possible modulation of the host response during OsHV-1 infection. We predicted 68 interactions between DE-miRNAs and oyster 3′-UTRs, but only few of them involved antiviral genes. The sncRNA reads assigned to OsHV-1 rather resembled mRNA degradation products, suggesting the absence of genuine viral miRNAs. Conclusions: We provided data describing the miRNAome during OsHV-1 infection in C. gigas. This information can be used to understand the role of miRNAs in healthy and diseased oysters, to identify new targets for functional studies and, eventually to disentangle cause and effect relationships during viral infections in marine mollusks. |
author2 |
Rosani, Umberto Abbadi, Miriam Green, Timothy Bai, Chang-Ming Turolla, Edoardo Arcangeli, Giuseppe Wegner K., Mathia Venier, Paola |
format |
Article in Journal/Newspaper |
author |
Rosani Umberto Abbadi Miriam Green Timothy Bai Chang-Ming Turolla Edoardo Arcangeli Giuseppe Wegner K. Mathias Venier Paola |
author_facet |
Rosani Umberto Abbadi Miriam Green Timothy Bai Chang-Ming Turolla Edoardo Arcangeli Giuseppe Wegner K. Mathias Venier Paola |
author_sort |
Rosani Umberto |
title |
Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 |
title_short |
Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 |
title_full |
Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 |
title_fullStr |
Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 |
title_full_unstemmed |
Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 |
title_sort |
parallel analysis of mirnas and mrnas suggests distinct regulatory networks in crassostrea gigas infected by ostreid herpesvirus 1 |
publisher |
BioMed Central (BMC) Part of Springer Nature |
publishDate |
2020 |
url |
http://hdl.handle.net/11577/3350559 https://doi.org/10.1186/s12864-020-07026-7 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-07026-7 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/32912133 info:eu-repo/semantics/altIdentifier/wos/WOS:000571763500002 volume:21 firstpage:1 lastpage:18 numberofpages:18 journal:BMC GENOMICS info:eu-repo/grantAgreement/EC/H2020/678589 http://hdl.handle.net/11577/3350559 doi:10.1186/s12864-020-07026-7 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85090818382 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-07026-7 |
op_doi |
https://doi.org/10.1186/s12864-020-07026-7 |
container_title |
BMC Genomics |
container_volume |
21 |
container_issue |
1 |
_version_ |
1796941101931692032 |