Morphological and molecular characterization of adults and larvae of Crassicauda spp. (Nematoda: Spirurida) from Mediterranean fin whales Balaenoptera physalus (Linnaeus, 1758)
Crassicauda boopis is known to infect the kidneys and vascular system of mysticetes included Balaenoptera physalus and has been recently reported in Mediterranean waters. Identification at the species level relies on the observation of morphological features of the adult parasites, but field conditi...
Published in: | International Journal for Parasitology: Parasites and Wildlife |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Australian Society for Parasitology
2019
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Online Access: | http://hdl.handle.net/11577/3313906 https://doi.org/10.1016/j.ijppaw.2019.06.004 http://www.journals.elsevier.com/international-journal-for-parasitology-parasites-and-wildlife/ |
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ftunivpadovairis:oai:www.research.unipd.it:11577/3313906 2024-04-14T08:09:30+00:00 Morphological and molecular characterization of adults and larvae of Crassicauda spp. (Nematoda: Spirurida) from Mediterranean fin whales Balaenoptera physalus (Linnaeus, 1758) Marcer F. Negrisolo E. Franzo G. Tessarin C. Pietrobelli M. Marchiori E. Marcer, F. Negrisolo, E. Franzo, G. Tessarin, C. Pietrobelli, M. Marchiori, E. 2019 ELETTRONICO http://hdl.handle.net/11577/3313906 https://doi.org/10.1016/j.ijppaw.2019.06.004 http://www.journals.elsevier.com/international-journal-for-parasitology-parasites-and-wildlife/ eng eng Australian Society for Parasitology info:eu-repo/semantics/altIdentifier/pmid/31341767 info:eu-repo/semantics/altIdentifier/wos/WOS:000474905300038 volume:9 firstpage:258 lastpage:265 numberofpages:8 journal:INTERNATIONAL JOURNAL FOR PARASITOLOGY. PARASITES AND WILDLIFE http://hdl.handle.net/11577/3313906 doi:10.1016/j.ijppaw.2019.06.004 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85067180516 http://www.journals.elsevier.com/international-journal-for-parasitology-parasites-and-wildlife/ info:eu-repo/semantics/openAccess Balaenoptera physalu cox1 Crassicauda Fin whale ITS-2 Nematoda info:eu-repo/semantics/article 2019 ftunivpadovairis https://doi.org/10.1016/j.ijppaw.2019.06.004 2024-03-21T19:37:06Z Crassicauda boopis is known to infect the kidneys and vascular system of mysticetes included Balaenoptera physalus and has been recently reported in Mediterranean waters. Identification at the species level relies on the observation of morphological features of the adult parasites, but field conditions during necropsy and the massive reaction of the host's immune system often prevent optimal conservation of the extremities. Moreover, larval stages of Crassicauda have never been described and no sequences are available in public databases to help such identification. Adult and larvae of Crassicauda were isolated from four specimens of B. physalus and studied with morphological and molecular techniques. Specimens of C. anthonyi, C. grampicola and Crassicauda sp. isolated from Ziphius cavirostris, Grampus griseus, Stenella coeruleoalba and Tursiops truncatus respectively were studied as well. Sequences of nuclear markers 18S and ITS-2 and of mitochondrial gene cox1 were obtained and phylogenetic relationships within the genus Crassicauda were analysed. Analysis of the ITS2 grouped the dif- ferent species in accordance with morphological identification, as already evidenced in literature for other Spirurida. A higher intra-specific variability was observed for the cox1 gene, for which two species (C. grampicola and C. anthonyi) did not appear as monophyletic in the tree. Well-developed non-attached larval specimens in the intestinal lumen of a whale calf were molecularly identified as C. boopis, allowing new insights on the life cycle of this species. This work broadens the genetic database on cetaceans parasites, allowing species identi- fication even in challenging field conditions or in poor conservation of the samples; moreover, the first mor- phological description of C. boopis larvae is provided. Article in Journal/Newspaper Balaenoptera physalus Fin whale Padua Research Archive (IRIS - Università degli Studi di Padova) International Journal for Parasitology: Parasites and Wildlife 9 258 265 |
institution |
Open Polar |
collection |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
op_collection_id |
ftunivpadovairis |
language |
English |
topic |
Balaenoptera physalu cox1 Crassicauda Fin whale ITS-2 Nematoda |
spellingShingle |
Balaenoptera physalu cox1 Crassicauda Fin whale ITS-2 Nematoda Marcer F. Negrisolo E. Franzo G. Tessarin C. Pietrobelli M. Marchiori E. Morphological and molecular characterization of adults and larvae of Crassicauda spp. (Nematoda: Spirurida) from Mediterranean fin whales Balaenoptera physalus (Linnaeus, 1758) |
topic_facet |
Balaenoptera physalu cox1 Crassicauda Fin whale ITS-2 Nematoda |
description |
Crassicauda boopis is known to infect the kidneys and vascular system of mysticetes included Balaenoptera physalus and has been recently reported in Mediterranean waters. Identification at the species level relies on the observation of morphological features of the adult parasites, but field conditions during necropsy and the massive reaction of the host's immune system often prevent optimal conservation of the extremities. Moreover, larval stages of Crassicauda have never been described and no sequences are available in public databases to help such identification. Adult and larvae of Crassicauda were isolated from four specimens of B. physalus and studied with morphological and molecular techniques. Specimens of C. anthonyi, C. grampicola and Crassicauda sp. isolated from Ziphius cavirostris, Grampus griseus, Stenella coeruleoalba and Tursiops truncatus respectively were studied as well. Sequences of nuclear markers 18S and ITS-2 and of mitochondrial gene cox1 were obtained and phylogenetic relationships within the genus Crassicauda were analysed. Analysis of the ITS2 grouped the dif- ferent species in accordance with morphological identification, as already evidenced in literature for other Spirurida. A higher intra-specific variability was observed for the cox1 gene, for which two species (C. grampicola and C. anthonyi) did not appear as monophyletic in the tree. Well-developed non-attached larval specimens in the intestinal lumen of a whale calf were molecularly identified as C. boopis, allowing new insights on the life cycle of this species. This work broadens the genetic database on cetaceans parasites, allowing species identi- fication even in challenging field conditions or in poor conservation of the samples; moreover, the first mor- phological description of C. boopis larvae is provided. |
author2 |
Marcer, F. Negrisolo, E. Franzo, G. Tessarin, C. Pietrobelli, M. Marchiori, E. |
format |
Article in Journal/Newspaper |
author |
Marcer F. Negrisolo E. Franzo G. Tessarin C. Pietrobelli M. Marchiori E. |
author_facet |
Marcer F. Negrisolo E. Franzo G. Tessarin C. Pietrobelli M. Marchiori E. |
author_sort |
Marcer F. |
title |
Morphological and molecular characterization of adults and larvae of Crassicauda spp. (Nematoda: Spirurida) from Mediterranean fin whales Balaenoptera physalus (Linnaeus, 1758) |
title_short |
Morphological and molecular characterization of adults and larvae of Crassicauda spp. (Nematoda: Spirurida) from Mediterranean fin whales Balaenoptera physalus (Linnaeus, 1758) |
title_full |
Morphological and molecular characterization of adults and larvae of Crassicauda spp. (Nematoda: Spirurida) from Mediterranean fin whales Balaenoptera physalus (Linnaeus, 1758) |
title_fullStr |
Morphological and molecular characterization of adults and larvae of Crassicauda spp. (Nematoda: Spirurida) from Mediterranean fin whales Balaenoptera physalus (Linnaeus, 1758) |
title_full_unstemmed |
Morphological and molecular characterization of adults and larvae of Crassicauda spp. (Nematoda: Spirurida) from Mediterranean fin whales Balaenoptera physalus (Linnaeus, 1758) |
title_sort |
morphological and molecular characterization of adults and larvae of crassicauda spp. (nematoda: spirurida) from mediterranean fin whales balaenoptera physalus (linnaeus, 1758) |
publisher |
Australian Society for Parasitology |
publishDate |
2019 |
url |
http://hdl.handle.net/11577/3313906 https://doi.org/10.1016/j.ijppaw.2019.06.004 http://www.journals.elsevier.com/international-journal-for-parasitology-parasites-and-wildlife/ |
genre |
Balaenoptera physalus Fin whale |
genre_facet |
Balaenoptera physalus Fin whale |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/31341767 info:eu-repo/semantics/altIdentifier/wos/WOS:000474905300038 volume:9 firstpage:258 lastpage:265 numberofpages:8 journal:INTERNATIONAL JOURNAL FOR PARASITOLOGY. PARASITES AND WILDLIFE http://hdl.handle.net/11577/3313906 doi:10.1016/j.ijppaw.2019.06.004 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85067180516 http://www.journals.elsevier.com/international-journal-for-parasitology-parasites-and-wildlife/ |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.ijppaw.2019.06.004 |
container_title |
International Journal for Parasitology: Parasites and Wildlife |
container_volume |
9 |
container_start_page |
258 |
op_container_end_page |
265 |
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1796306987899682816 |