The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect
Voltage-dependent anion channel (VDAC) is a key mitochondrial protein. VDAC drives cellular energy metabolism by controlling the influx and efflux of metabolites and ions through the mitochondrial membrane, playing a role in its permeabilization. This protein exerts a pivotal role during the white s...
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Online Access: | http://hdl.handle.net/11577/3254685 https://doi.org/10.1007/s10126-017-9789-x |
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ftunivpadovairis:oai:www.research.unipd.it:11577/3254685 2024-04-14T08:10:39+00:00 The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect Delisle, Lizenn Fuhrmann, Marine Quéré, Claudie Pauletto, Marianna Pichereau, Vianney Pernet, Fabrice Corporeau, Charlotte Delisle, Lizenn Fuhrmann, Marine Quéré, Claudie Pauletto, Marianna Pichereau, Vianney Pernet, Fabrice Corporeau, Charlotte 2018 STAMPA http://hdl.handle.net/11577/3254685 https://doi.org/10.1007/s10126-017-9789-x eng eng info:eu-repo/semantics/altIdentifier/pmid/29344825 info:eu-repo/semantics/altIdentifier/wos/WOS:000424459500008 journal:MARINE BIOTECHNOLOGY http://hdl.handle.net/11577/3254685 doi:10.1007/s10126-017-9789-x info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85040692929 info:eu-repo/semantics/openAccess Crassostrea giga Ostreid herpes viru Voltage-dependent anion channel Warburg effect info:eu-repo/semantics/article 2018 ftunivpadovairis https://doi.org/10.1007/s10126-017-9789-x 2024-03-21T19:29:50Z Voltage-dependent anion channel (VDAC) is a key mitochondrial protein. VDAC drives cellular energy metabolism by controlling the influx and efflux of metabolites and ions through the mitochondrial membrane, playing a role in its permeabilization. This protein exerts a pivotal role during the white spot syndrome virus (WSSV) infection in shrimp, through its involvement in a particular metabolism that plays in favor of the virus, the Warburg effect. The Warburg effect corresponds to an atypical metabolic shift toward an aerobic glycolysis that provides energy for rapid cell division and resistance to apoptosis. In the Pacific oyster Crassostrea gigas, the Warburg effect occurs during infection by Ostreid herpesvirus (OsHV-1). At present, the role of VDAC in the Warburg effect, OsHV-1 infection and apoptosis is unknown. Here, we developed a specific antibody directed against C. gigas VDAC. This tool allowed us to quantify the tissue-specific expression of VDAC, to detect VDAC oligomers, and to follow the amount of VDAC in oysters deployed in the field. We showed that oysters sensitive to a mortality event in the field presented an accumulation of VDAC. Finally, we propose to use VDAC quantification as a tool to measure the oyster susceptibility to OsHV-1 depending on its environment. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Padua Research Archive (IRIS - Università degli Studi di Padova) Pacific Marine Biotechnology 20 1 87 97 |
institution |
Open Polar |
collection |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
op_collection_id |
ftunivpadovairis |
language |
English |
topic |
Crassostrea giga Ostreid herpes viru Voltage-dependent anion channel Warburg effect |
spellingShingle |
Crassostrea giga Ostreid herpes viru Voltage-dependent anion channel Warburg effect Delisle, Lizenn Fuhrmann, Marine Quéré, Claudie Pauletto, Marianna Pichereau, Vianney Pernet, Fabrice Corporeau, Charlotte The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect |
topic_facet |
Crassostrea giga Ostreid herpes viru Voltage-dependent anion channel Warburg effect |
description |
Voltage-dependent anion channel (VDAC) is a key mitochondrial protein. VDAC drives cellular energy metabolism by controlling the influx and efflux of metabolites and ions through the mitochondrial membrane, playing a role in its permeabilization. This protein exerts a pivotal role during the white spot syndrome virus (WSSV) infection in shrimp, through its involvement in a particular metabolism that plays in favor of the virus, the Warburg effect. The Warburg effect corresponds to an atypical metabolic shift toward an aerobic glycolysis that provides energy for rapid cell division and resistance to apoptosis. In the Pacific oyster Crassostrea gigas, the Warburg effect occurs during infection by Ostreid herpesvirus (OsHV-1). At present, the role of VDAC in the Warburg effect, OsHV-1 infection and apoptosis is unknown. Here, we developed a specific antibody directed against C. gigas VDAC. This tool allowed us to quantify the tissue-specific expression of VDAC, to detect VDAC oligomers, and to follow the amount of VDAC in oysters deployed in the field. We showed that oysters sensitive to a mortality event in the field presented an accumulation of VDAC. Finally, we propose to use VDAC quantification as a tool to measure the oyster susceptibility to OsHV-1 depending on its environment. |
author2 |
Delisle, Lizenn Fuhrmann, Marine Quéré, Claudie Pauletto, Marianna Pichereau, Vianney Pernet, Fabrice Corporeau, Charlotte |
format |
Article in Journal/Newspaper |
author |
Delisle, Lizenn Fuhrmann, Marine Quéré, Claudie Pauletto, Marianna Pichereau, Vianney Pernet, Fabrice Corporeau, Charlotte |
author_facet |
Delisle, Lizenn Fuhrmann, Marine Quéré, Claudie Pauletto, Marianna Pichereau, Vianney Pernet, Fabrice Corporeau, Charlotte |
author_sort |
Delisle, Lizenn |
title |
The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect |
title_short |
The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect |
title_full |
The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect |
title_fullStr |
The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect |
title_full_unstemmed |
The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect |
title_sort |
voltage-dependent anion channel (vdac) of pacific oysters crassostrea gigas is upaccumulated during infection by the ostreid herpesvirus-1 (oshv-1): an indicator of the warburg effect |
publishDate |
2018 |
url |
http://hdl.handle.net/11577/3254685 https://doi.org/10.1007/s10126-017-9789-x |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/29344825 info:eu-repo/semantics/altIdentifier/wos/WOS:000424459500008 journal:MARINE BIOTECHNOLOGY http://hdl.handle.net/11577/3254685 doi:10.1007/s10126-017-9789-x info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85040692929 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1007/s10126-017-9789-x |
container_title |
Marine Biotechnology |
container_volume |
20 |
container_issue |
1 |
container_start_page |
87 |
op_container_end_page |
97 |
_version_ |
1796308289329299456 |