Functional characterization of the circadian clock in the Antarctic krill, Euphausia superba
Antarctic krill (Euphausia superba) is a key species in Southern Ocean ecosystem where it plays a central role in the Antarctic food web. Available information supports the existence of an endogenous timing system in krill enabling it to synchronize metabolism and behavior with an environment charac...
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ftunivpadovairis:oai:www.research.unipd.it:11577/3254543 2024-04-14T08:04:40+00:00 Functional characterization of the circadian clock in the Antarctic krill, Euphausia superba Biscontin, Alberto Wallach, Thomas Sales, Gabriele Grudziecki, Astrid Janke, Leonard Sartori, Elena Bertolucci, Cristiano Mazzotta, Gabriella De Pittà , Cristiano Meyer, Bettina Kramer, Achim Costa, Rodolfo Biscontin, Alberto Wallach, Thoma Sales, Gabriele Grudziecki, Astrid Janke, Leonard Sartori, Elena Bertolucci, Cristiano Mazzotta, Gabriella De Pittà , Cristiano Meyer, Bettina Kramer, Achim Costa, Rodolfo 2017 ELETTRONICO http://hdl.handle.net/11577/3254543 https://doi.org/10.1038/s41598-017-18009-2 eng eng Nature Publishing Group info:eu-repo/semantics/altIdentifier/pmid/29255161 info:eu-repo/semantics/altIdentifier/wos/WOS:000418323900054 volume:7 issue:1 firstpage:17742 journal:SCIENTIFIC REPORTS http://hdl.handle.net/11577/3254543 doi:10.1038/s41598-017-18009-2 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85038568055 www.nature.com/srep/index.html info:eu-repo/semantics/openAccess Circadian mechanisms Gene regulation Euphausia superba info:eu-repo/semantics/article 2017 ftunivpadovairis https://doi.org/10.1038/s41598-017-18009-2 2024-03-21T19:29:50Z Antarctic krill (Euphausia superba) is a key species in Southern Ocean ecosystem where it plays a central role in the Antarctic food web. Available information supports the existence of an endogenous timing system in krill enabling it to synchronize metabolism and behavior with an environment characterized by extreme seasonal changes in terms of day length, food availability, and surface ice extent. A screening of our transcriptome database "KrillDB" allowed us to identify the putative orthologues of 20 circadian clock components. Mapping of conserved domains and phylogenetic analyses strongly supported annotations of the identified sequences. Luciferase assays and co-immunoprecipitation experiments allowed us to define the role of the main clock components. Our findings provide an overall picture of the molecular mechanisms underlying the functioning of the endogenous circadian clock in the Antarctic krill and shed light on their evolution throughout crustaceans speciation. Interestingly, the core clock machinery shows both mammalian and insect features that presumably contribute to an evolutionary strategy to cope with polar environment's challenges. Moreover, despite the extreme variability characterizing the Antarctic seasonal day length, the conserved light mediated degradation of the photoreceptor EsCRY1 suggests a persisting pivotal role of light as a Zeitgeber. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Euphausia superba Southern Ocean Padua Research Archive (IRIS - Università degli Studi di Padova) Antarctic Southern Ocean The Antarctic Scientific Reports 7 1 |
institution |
Open Polar |
collection |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
op_collection_id |
ftunivpadovairis |
language |
English |
topic |
Circadian mechanisms Gene regulation Euphausia superba |
spellingShingle |
Circadian mechanisms Gene regulation Euphausia superba Biscontin, Alberto Wallach, Thomas Sales, Gabriele Grudziecki, Astrid Janke, Leonard Sartori, Elena Bertolucci, Cristiano Mazzotta, Gabriella De Pittà , Cristiano Meyer, Bettina Kramer, Achim Costa, Rodolfo Functional characterization of the circadian clock in the Antarctic krill, Euphausia superba |
topic_facet |
Circadian mechanisms Gene regulation Euphausia superba |
description |
Antarctic krill (Euphausia superba) is a key species in Southern Ocean ecosystem where it plays a central role in the Antarctic food web. Available information supports the existence of an endogenous timing system in krill enabling it to synchronize metabolism and behavior with an environment characterized by extreme seasonal changes in terms of day length, food availability, and surface ice extent. A screening of our transcriptome database "KrillDB" allowed us to identify the putative orthologues of 20 circadian clock components. Mapping of conserved domains and phylogenetic analyses strongly supported annotations of the identified sequences. Luciferase assays and co-immunoprecipitation experiments allowed us to define the role of the main clock components. Our findings provide an overall picture of the molecular mechanisms underlying the functioning of the endogenous circadian clock in the Antarctic krill and shed light on their evolution throughout crustaceans speciation. Interestingly, the core clock machinery shows both mammalian and insect features that presumably contribute to an evolutionary strategy to cope with polar environment's challenges. Moreover, despite the extreme variability characterizing the Antarctic seasonal day length, the conserved light mediated degradation of the photoreceptor EsCRY1 suggests a persisting pivotal role of light as a Zeitgeber. |
author2 |
Biscontin, Alberto Wallach, Thoma Sales, Gabriele Grudziecki, Astrid Janke, Leonard Sartori, Elena Bertolucci, Cristiano Mazzotta, Gabriella De Pittà , Cristiano Meyer, Bettina Kramer, Achim Costa, Rodolfo |
format |
Article in Journal/Newspaper |
author |
Biscontin, Alberto Wallach, Thomas Sales, Gabriele Grudziecki, Astrid Janke, Leonard Sartori, Elena Bertolucci, Cristiano Mazzotta, Gabriella De Pittà , Cristiano Meyer, Bettina Kramer, Achim Costa, Rodolfo |
author_facet |
Biscontin, Alberto Wallach, Thomas Sales, Gabriele Grudziecki, Astrid Janke, Leonard Sartori, Elena Bertolucci, Cristiano Mazzotta, Gabriella De Pittà , Cristiano Meyer, Bettina Kramer, Achim Costa, Rodolfo |
author_sort |
Biscontin, Alberto |
title |
Functional characterization of the circadian clock in the Antarctic krill, Euphausia superba |
title_short |
Functional characterization of the circadian clock in the Antarctic krill, Euphausia superba |
title_full |
Functional characterization of the circadian clock in the Antarctic krill, Euphausia superba |
title_fullStr |
Functional characterization of the circadian clock in the Antarctic krill, Euphausia superba |
title_full_unstemmed |
Functional characterization of the circadian clock in the Antarctic krill, Euphausia superba |
title_sort |
functional characterization of the circadian clock in the antarctic krill, euphausia superba |
publisher |
Nature Publishing Group |
publishDate |
2017 |
url |
http://hdl.handle.net/11577/3254543 https://doi.org/10.1038/s41598-017-18009-2 |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba Southern Ocean |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/29255161 info:eu-repo/semantics/altIdentifier/wos/WOS:000418323900054 volume:7 issue:1 firstpage:17742 journal:SCIENTIFIC REPORTS http://hdl.handle.net/11577/3254543 doi:10.1038/s41598-017-18009-2 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85038568055 www.nature.com/srep/index.html |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1038/s41598-017-18009-2 |
container_title |
Scientific Reports |
container_volume |
7 |
container_issue |
1 |
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1796301378567536640 |