Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication
Double stranded RNA-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve, i.e. Crassostrea gigas, as well as the specific ro...
Published in: | Journal of Experimental Biology |
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Online Access: | http://hdl.handle.net/11577/3240320 https://doi.org/10.1242/jeb.156299 http://jeb.biologists.org/content/early/2017/08/09/jeb.156299.long |
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ftunivpadovairis:oai:www.research.unipd.it:11577/3240320 2024-04-21T08:00:46+00:00 Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication PAULETTO, MARIANNA Segarra, Amélie Montagnani, Caroline Quillien, Virgile Faury, Nicole Le Grand, Jacqueline Miner, Philippe Petton, Bruno Labreuche, Yannick Fleury, Elodie Fabioux, Caroline BARGELLONI, LUCA Renault, Tristan Huvet, Arnaud Pauletto, Marianna Segarra, Amélie Montagnani, Caroline Quillien, Virgile Faury, Nicole Le Grand, Jacqueline Miner, Philippe Petton, Bruno Labreuche, Yannick Fleury, Elodie Fabioux, Caroline Bargelloni, Luca Renault, Tristan Huvet, Arnaud 2017 STAMPA http://hdl.handle.net/11577/3240320 https://doi.org/10.1242/jeb.156299 http://jeb.biologists.org/content/early/2017/08/09/jeb.156299.long eng eng info:eu-repo/semantics/altIdentifier/pmid/28798082 info:eu-repo/semantics/altIdentifier/wos/WOS:000413196900013 firstpage:jeb.156299 journal:JOURNAL OF EXPERIMENTAL BIOLOGY http://hdl.handle.net/11577/3240320 doi:10.1242/jeb.156299 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85031683469 http://jeb.biologists.org/content/early/2017/08/09/jeb.156299.long info:eu-repo/semantics/openAccess Anti-viral response Inhibitor of NF-κB Marine bivalve Ostreid herpesvirus 1 RNA interference info:eu-repo/semantics/article 2017 ftunivpadovairis https://doi.org/10.1242/jeb.156299 2024-03-28T01:59:09Z Double stranded RNA-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve, i.e. Crassostrea gigas, as well as the specific role of the subunit 2 of the nuclear factor κB inhibitor (IκB2). This gene is a candidate of particular interest for functional investigations in the context of massive mortality oyster events as Cg-IκB2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-IκB2 and Green Fluorescence Protein genes were injected in vivo into oysters before being challenged by OsHV-1. Survival appeared close to 100% in both dsRNA injected conditions associated with a low detection of viral DNA and a low expression of a panel of 39 OsHV-1 genes as compared to infected control. Long dsRNA molecules, both Cg-IκB2- and GFP-dsRNA, may have induced an anti-viral state controlling the OsHV-1 replication and precluding the understanding of the Cg-IκB2 specific role. Immune-related genes including Cg-IκB1, Cg-Rel1, Cg-IFI44, Cg-PKR, and Cg-IAP appeared activated in dsRNA-injected condition potentially hampering viral replication and thus conferring a better resistance to OsHV-1 infection. We revealed that long dsRNA-mediated genetic interference triggered an anti-viral state in the oyster, emphasizing the need of new reverse genetics tools for assessing immune gene function and avoiding off-target effects in bivalves. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Padua Research Archive (IRIS - Università degli Studi di Padova) Journal of Experimental Biology |
institution |
Open Polar |
collection |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
op_collection_id |
ftunivpadovairis |
language |
English |
topic |
Anti-viral response Inhibitor of NF-κB Marine bivalve Ostreid herpesvirus 1 RNA interference |
spellingShingle |
Anti-viral response Inhibitor of NF-κB Marine bivalve Ostreid herpesvirus 1 RNA interference PAULETTO, MARIANNA Segarra, Amélie Montagnani, Caroline Quillien, Virgile Faury, Nicole Le Grand, Jacqueline Miner, Philippe Petton, Bruno Labreuche, Yannick Fleury, Elodie Fabioux, Caroline BARGELLONI, LUCA Renault, Tristan Huvet, Arnaud Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication |
topic_facet |
Anti-viral response Inhibitor of NF-κB Marine bivalve Ostreid herpesvirus 1 RNA interference |
description |
Double stranded RNA-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve, i.e. Crassostrea gigas, as well as the specific role of the subunit 2 of the nuclear factor κB inhibitor (IκB2). This gene is a candidate of particular interest for functional investigations in the context of massive mortality oyster events as Cg-IκB2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-IκB2 and Green Fluorescence Protein genes were injected in vivo into oysters before being challenged by OsHV-1. Survival appeared close to 100% in both dsRNA injected conditions associated with a low detection of viral DNA and a low expression of a panel of 39 OsHV-1 genes as compared to infected control. Long dsRNA molecules, both Cg-IκB2- and GFP-dsRNA, may have induced an anti-viral state controlling the OsHV-1 replication and precluding the understanding of the Cg-IκB2 specific role. Immune-related genes including Cg-IκB1, Cg-Rel1, Cg-IFI44, Cg-PKR, and Cg-IAP appeared activated in dsRNA-injected condition potentially hampering viral replication and thus conferring a better resistance to OsHV-1 infection. We revealed that long dsRNA-mediated genetic interference triggered an anti-viral state in the oyster, emphasizing the need of new reverse genetics tools for assessing immune gene function and avoiding off-target effects in bivalves. |
author2 |
Pauletto, Marianna Segarra, Amélie Montagnani, Caroline Quillien, Virgile Faury, Nicole Le Grand, Jacqueline Miner, Philippe Petton, Bruno Labreuche, Yannick Fleury, Elodie Fabioux, Caroline Bargelloni, Luca Renault, Tristan Huvet, Arnaud |
format |
Article in Journal/Newspaper |
author |
PAULETTO, MARIANNA Segarra, Amélie Montagnani, Caroline Quillien, Virgile Faury, Nicole Le Grand, Jacqueline Miner, Philippe Petton, Bruno Labreuche, Yannick Fleury, Elodie Fabioux, Caroline BARGELLONI, LUCA Renault, Tristan Huvet, Arnaud |
author_facet |
PAULETTO, MARIANNA Segarra, Amélie Montagnani, Caroline Quillien, Virgile Faury, Nicole Le Grand, Jacqueline Miner, Philippe Petton, Bruno Labreuche, Yannick Fleury, Elodie Fabioux, Caroline BARGELLONI, LUCA Renault, Tristan Huvet, Arnaud |
author_sort |
PAULETTO, MARIANNA |
title |
Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication |
title_short |
Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication |
title_full |
Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication |
title_fullStr |
Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication |
title_full_unstemmed |
Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication |
title_sort |
long dsrnas promote an anti-viral response in pacific oyster hampering ostreid herpesvirus 1 replication |
publishDate |
2017 |
url |
http://hdl.handle.net/11577/3240320 https://doi.org/10.1242/jeb.156299 http://jeb.biologists.org/content/early/2017/08/09/jeb.156299.long |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/28798082 info:eu-repo/semantics/altIdentifier/wos/WOS:000413196900013 firstpage:jeb.156299 journal:JOURNAL OF EXPERIMENTAL BIOLOGY http://hdl.handle.net/11577/3240320 doi:10.1242/jeb.156299 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85031683469 http://jeb.biologists.org/content/early/2017/08/09/jeb.156299.long |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1242/jeb.156299 |
container_title |
Journal of Experimental Biology |
_version_ |
1796941196451381248 |