Fast genetic identification of the Beluga sturgeon and its sought-after caviar to stem illegal trade
Sturgeons are well known for the delicacy of their eggs, the caviar, one of the most valuable products on the food market. The high price of caviar led in the past to a severe overharvest of wild sturgeon species and to an increase in trade of counterfeit products sold with impunity in spite of the...
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Online Access: | http://hdl.handle.net/11577/3224688 https://doi.org/10.1016/j.foodcont.2016.11.039 |
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ftunivpadovairis:oai:www.research.unipd.it:11577/3224688 2024-02-11T10:02:30+01:00 Fast genetic identification of the Beluga sturgeon and its sought-after caviar to stem illegal trade BOSCARI, ELISA VITULO, NICOLA Ludwig, Arne CARUSO, CHIARA Mugue, Nikolai S. Suciu, Radu Onara, Dalia F. PAPETTI, CHIARA MARINO, ILARIA ANNA MARIA ZANE, LORENZO CONGIU, LEONARDO Boscari, Elisa Vitulo, Nicola Ludwig, Arne Caruso, Chiara Mugue, Nikolai S. Suciu, Radu Onara, Dalia F. Papetti, Chiara Marino, ILARIA ANNA MARIA Zane, Lorenzo Congiu, Leonardo 2017 STAMPA http://hdl.handle.net/11577/3224688 https://doi.org/10.1016/j.foodcont.2016.11.039 eng eng Elsevier Ltd info:eu-repo/semantics/altIdentifier/wos/WOS:000393009700018 volume:75 firstpage:145 lastpage:152 numberofpages:8 journal:FOOD CONTROL http://hdl.handle.net/11577/3224688 doi:10.1016/j.foodcont.2016.11.039 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85008157840 Bester Huso huso Hybrid RP2S6 Species identification Sturgeon Biotechnology Food Science info:eu-repo/semantics/article 2017 ftunivpadovairis https://doi.org/10.1016/j.foodcont.2016.11.039 2024-01-24T17:45:01Z Sturgeons are well known for the delicacy of their eggs, the caviar, one of the most valuable products on the food market. The high price of caviar led in the past to a severe overharvest of wild sturgeon species and to an increase in trade of counterfeit products sold with impunity in spite of the strict trade limitations. A priority in the effort to reduce illegal trading is the development of genetic tools in order to identify the species of traded products using a standardized, cheap and rapid approach. We developed the first genetic nuclear marker for the identification of the Beluga sturgeon (Huso huso), the most sought-after caviar producer. We explored the interspecific variability at the setond intron of the nuclear S6 Ribosomal Protein (RP2S6), selected among 1867 introns, predicted by aligning the transcriptome of 3 sturgeon species with 3 complete fish genomes. The Beluga-specific SNP was identified by cloning and sequencing RP2S6 in 65 individuals of 11 species, validated on 341 additional individuals and tested on 18 caviar samples. Diagnostic primers designed on the SNP successfully amplified the expected band in all Beluga specimens while no PCR product was obtained from other pure species. The marker can also contribute to the identification of interspecific hybrids in which the Beluga is one of the parent species, such as in the case of the Bester, which produces one of the most mislabeled caviars in trade. The complete identification power on this highly relevant species and the proved efficacy on caviar samples represent an essential progress towards a standardized panel of nuclear markers for the control of illegal poaching, smuggling and mislabeling of sturgeons and their products. Article in Journal/Newspaper Beluga Beluga* Padua Research Archive (IRIS - Università degli Studi di Padova) Food Control 75 145 152 |
institution |
Open Polar |
collection |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
op_collection_id |
ftunivpadovairis |
language |
English |
topic |
Bester Huso huso Hybrid RP2S6 Species identification Sturgeon Biotechnology Food Science |
spellingShingle |
Bester Huso huso Hybrid RP2S6 Species identification Sturgeon Biotechnology Food Science BOSCARI, ELISA VITULO, NICOLA Ludwig, Arne CARUSO, CHIARA Mugue, Nikolai S. Suciu, Radu Onara, Dalia F. PAPETTI, CHIARA MARINO, ILARIA ANNA MARIA ZANE, LORENZO CONGIU, LEONARDO Fast genetic identification of the Beluga sturgeon and its sought-after caviar to stem illegal trade |
topic_facet |
Bester Huso huso Hybrid RP2S6 Species identification Sturgeon Biotechnology Food Science |
description |
Sturgeons are well known for the delicacy of their eggs, the caviar, one of the most valuable products on the food market. The high price of caviar led in the past to a severe overharvest of wild sturgeon species and to an increase in trade of counterfeit products sold with impunity in spite of the strict trade limitations. A priority in the effort to reduce illegal trading is the development of genetic tools in order to identify the species of traded products using a standardized, cheap and rapid approach. We developed the first genetic nuclear marker for the identification of the Beluga sturgeon (Huso huso), the most sought-after caviar producer. We explored the interspecific variability at the setond intron of the nuclear S6 Ribosomal Protein (RP2S6), selected among 1867 introns, predicted by aligning the transcriptome of 3 sturgeon species with 3 complete fish genomes. The Beluga-specific SNP was identified by cloning and sequencing RP2S6 in 65 individuals of 11 species, validated on 341 additional individuals and tested on 18 caviar samples. Diagnostic primers designed on the SNP successfully amplified the expected band in all Beluga specimens while no PCR product was obtained from other pure species. The marker can also contribute to the identification of interspecific hybrids in which the Beluga is one of the parent species, such as in the case of the Bester, which produces one of the most mislabeled caviars in trade. The complete identification power on this highly relevant species and the proved efficacy on caviar samples represent an essential progress towards a standardized panel of nuclear markers for the control of illegal poaching, smuggling and mislabeling of sturgeons and their products. |
author2 |
Boscari, Elisa Vitulo, Nicola Ludwig, Arne Caruso, Chiara Mugue, Nikolai S. Suciu, Radu Onara, Dalia F. Papetti, Chiara Marino, ILARIA ANNA MARIA Zane, Lorenzo Congiu, Leonardo |
format |
Article in Journal/Newspaper |
author |
BOSCARI, ELISA VITULO, NICOLA Ludwig, Arne CARUSO, CHIARA Mugue, Nikolai S. Suciu, Radu Onara, Dalia F. PAPETTI, CHIARA MARINO, ILARIA ANNA MARIA ZANE, LORENZO CONGIU, LEONARDO |
author_facet |
BOSCARI, ELISA VITULO, NICOLA Ludwig, Arne CARUSO, CHIARA Mugue, Nikolai S. Suciu, Radu Onara, Dalia F. PAPETTI, CHIARA MARINO, ILARIA ANNA MARIA ZANE, LORENZO CONGIU, LEONARDO |
author_sort |
BOSCARI, ELISA |
title |
Fast genetic identification of the Beluga sturgeon and its sought-after caviar to stem illegal trade |
title_short |
Fast genetic identification of the Beluga sturgeon and its sought-after caviar to stem illegal trade |
title_full |
Fast genetic identification of the Beluga sturgeon and its sought-after caviar to stem illegal trade |
title_fullStr |
Fast genetic identification of the Beluga sturgeon and its sought-after caviar to stem illegal trade |
title_full_unstemmed |
Fast genetic identification of the Beluga sturgeon and its sought-after caviar to stem illegal trade |
title_sort |
fast genetic identification of the beluga sturgeon and its sought-after caviar to stem illegal trade |
publisher |
Elsevier Ltd |
publishDate |
2017 |
url |
http://hdl.handle.net/11577/3224688 https://doi.org/10.1016/j.foodcont.2016.11.039 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000393009700018 volume:75 firstpage:145 lastpage:152 numberofpages:8 journal:FOOD CONTROL http://hdl.handle.net/11577/3224688 doi:10.1016/j.foodcont.2016.11.039 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85008157840 |
op_doi |
https://doi.org/10.1016/j.foodcont.2016.11.039 |
container_title |
Food Control |
container_volume |
75 |
container_start_page |
145 |
op_container_end_page |
152 |
_version_ |
1790598523865006080 |