IL-17 signaling components in bivalves: comparative sequence analysis and involvement in the immune responses
The recent discovery of soluble immune-regulatory molecules in invertebrates takes advantage of the rapid growth of next generation sequencing datasets. Following protein domain searches in the transcriptomes of 31 bivalve spp. and in few available mollusk genomes, we retrieved 59 domains uniquely i...
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ftunivpadovairis:oai:www.research.unipd.it:11577/3144127 2024-02-11T10:03:13+01:00 IL-17 signaling components in bivalves: comparative sequence analysis and involvement in the immune responses ROSANI, UMBERTO VAROTTO, LAURA VENIER, PAOLA Gerdol M Pallavicini A Rosani, Umberto Varotto, Laura Gerdol, M Pallavicini, A Venier, Paola 2015 STAMPA http://hdl.handle.net/11577/3144127 eng eng Elsevier info:eu-repo/semantics/altIdentifier/wos/WOS:000360592200016 volume:000 firstpage:00 lastpage:00 journal:DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://hdl.handle.net/11577/3144127 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84934914410 Mytilus galloprovinciali Interleukin 17 signaling pathway Crassostrea giga Bivalvia gene expression protein domains info:eu-repo/semantics/article 2015 ftunivpadovairis 2024-01-24T17:41:05Z The recent discovery of soluble immune-regulatory molecules in invertebrates takes advantage of the rapid growth of next generation sequencing datasets. Following protein domain searches in the transcriptomes of 31 bivalve spp. and in few available mollusk genomes, we retrieved 59 domains uniquely identifying interleukin 17 (IL-17) and 96 SEFIR domains typical of IL-17 receptors and CIKS/ACT1 proteins acting downstream in the IL-17 signaling pathway. Compared to the Chordata IL-17 family members, we confirm a separate clustering of the bivalve domain sequences and a consistent conservation pattern of amino acid residues. Analysis performed at transcript and genome level allowed us to propose an updated view of the components outlining the IL-17 signaling pathway in Mytilus galloprovincialis and Crassostrea gigas (in both species, homology modeling reduced the variety of IL-17 domains to only two 3D structures). Digital expression analysis indicated more heterogeneous expression levels for the mussel and oyster IL-17 ligands than for IL-17 receptors and CIKS/CIKSL proteins. Besides, new qPCR analyses confirmed such gene expression trends in hemocytes and gills of mussels challenged with heat-killed bacteria. These results uphold the involvement of an ancient IL-17 signaling pathway in the bivalve immune responses and, likewise in humans, suggest the possibility of distinctive modulatory roles of individual IL-17s/IL-17 receptors. Overall, the common evidence of pro-inflammatory cytokines and inter-related intracellular signaling pathways in bivalves definitely add complexity to the invertebrate immunity. Article in Journal/Newspaper Crassostrea gigas Padua Research Archive (IRIS - Università degli Studi di Padova) |
institution |
Open Polar |
collection |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
op_collection_id |
ftunivpadovairis |
language |
English |
topic |
Mytilus galloprovinciali Interleukin 17 signaling pathway Crassostrea giga Bivalvia gene expression protein domains |
spellingShingle |
Mytilus galloprovinciali Interleukin 17 signaling pathway Crassostrea giga Bivalvia gene expression protein domains ROSANI, UMBERTO VAROTTO, LAURA VENIER, PAOLA Gerdol M Pallavicini A IL-17 signaling components in bivalves: comparative sequence analysis and involvement in the immune responses |
topic_facet |
Mytilus galloprovinciali Interleukin 17 signaling pathway Crassostrea giga Bivalvia gene expression protein domains |
description |
The recent discovery of soluble immune-regulatory molecules in invertebrates takes advantage of the rapid growth of next generation sequencing datasets. Following protein domain searches in the transcriptomes of 31 bivalve spp. and in few available mollusk genomes, we retrieved 59 domains uniquely identifying interleukin 17 (IL-17) and 96 SEFIR domains typical of IL-17 receptors and CIKS/ACT1 proteins acting downstream in the IL-17 signaling pathway. Compared to the Chordata IL-17 family members, we confirm a separate clustering of the bivalve domain sequences and a consistent conservation pattern of amino acid residues. Analysis performed at transcript and genome level allowed us to propose an updated view of the components outlining the IL-17 signaling pathway in Mytilus galloprovincialis and Crassostrea gigas (in both species, homology modeling reduced the variety of IL-17 domains to only two 3D structures). Digital expression analysis indicated more heterogeneous expression levels for the mussel and oyster IL-17 ligands than for IL-17 receptors and CIKS/CIKSL proteins. Besides, new qPCR analyses confirmed such gene expression trends in hemocytes and gills of mussels challenged with heat-killed bacteria. These results uphold the involvement of an ancient IL-17 signaling pathway in the bivalve immune responses and, likewise in humans, suggest the possibility of distinctive modulatory roles of individual IL-17s/IL-17 receptors. Overall, the common evidence of pro-inflammatory cytokines and inter-related intracellular signaling pathways in bivalves definitely add complexity to the invertebrate immunity. |
author2 |
Rosani, Umberto Varotto, Laura Gerdol, M Pallavicini, A Venier, Paola |
format |
Article in Journal/Newspaper |
author |
ROSANI, UMBERTO VAROTTO, LAURA VENIER, PAOLA Gerdol M Pallavicini A |
author_facet |
ROSANI, UMBERTO VAROTTO, LAURA VENIER, PAOLA Gerdol M Pallavicini A |
author_sort |
ROSANI, UMBERTO |
title |
IL-17 signaling components in bivalves: comparative sequence analysis and involvement in the immune responses |
title_short |
IL-17 signaling components in bivalves: comparative sequence analysis and involvement in the immune responses |
title_full |
IL-17 signaling components in bivalves: comparative sequence analysis and involvement in the immune responses |
title_fullStr |
IL-17 signaling components in bivalves: comparative sequence analysis and involvement in the immune responses |
title_full_unstemmed |
IL-17 signaling components in bivalves: comparative sequence analysis and involvement in the immune responses |
title_sort |
il-17 signaling components in bivalves: comparative sequence analysis and involvement in the immune responses |
publisher |
Elsevier |
publishDate |
2015 |
url |
http://hdl.handle.net/11577/3144127 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000360592200016 volume:000 firstpage:00 lastpage:00 journal:DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://hdl.handle.net/11577/3144127 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84934914410 |
_version_ |
1790599415455547392 |