Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas
The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previou...
Published in: | Biology of Reproduction |
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Online Access: | http://hdl.handle.net/11577/2715286 https://doi.org/10.1095/biolreprod.113.109728 |
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ftunivpadovairis:oai:www.research.unipd.it:11577/2715286 2024-04-21T08:00:39+00:00 Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas E. Guevelou A. Huvet C. E. Galindo Sanchez V. Quillien J. Y. Daniel C. Quere P. Boudry C. Corporeau MILAN, MASSIMO E., Guevelou A., Huvet C. E., Galindo Sanchez Milan, Massimo V., Quillien J. Y., Daniel C., Quere P., Boudry C., Corporeau 2013 http://hdl.handle.net/11577/2715286 https://doi.org/10.1095/biolreprod.113.109728 eng eng info:eu-repo/semantics/altIdentifier/pmid/23926284 info:eu-repo/semantics/altIdentifier/wos/WOS:000326925800019 volume:89 firstpage:100 lastpage:100 journal:BIOLOGY OF REPRODUCTION http://hdl.handle.net/11577/2715286 doi:10.1095/biolreprod.113.109728 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84899788469 info:eu-repo/semantics/article 2013 ftunivpadovairis https://doi.org/10.1095/biolreprod.113.109728 2024-03-28T01:52:33Z The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the first stage of gametogenesis, when mitotic activity and the differentiation of germinal cells occur, and were always more abundant in males than in females. Some targets of fatty acid and glucose metabolism appeared to be correlated with the expression of AMPK subunits at the mRNA level. We then analyzed the sex-specific AMPK activity by measuring the phosphorylation of the catalytic AMPK alpha protein and its expression at the protein level. Both the amount of AMPK alpha protein and threonine 172 phosphorylation appeared to be almost totally inhibited in mature female gonads at stage 3, at the time when accumulation of reserves in oocytes was promoted, while it remained at a high level in mature spermatozoa. Its activation might play a sex-dependent role in the management of energy during gametogenesis in oyster. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Padua Research Archive (IRIS - Università degli Studi di Padova) Biology of Reproduction 89 4 |
institution |
Open Polar |
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Padua Research Archive (IRIS - Università degli Studi di Padova) |
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ftunivpadovairis |
language |
English |
description |
The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the first stage of gametogenesis, when mitotic activity and the differentiation of germinal cells occur, and were always more abundant in males than in females. Some targets of fatty acid and glucose metabolism appeared to be correlated with the expression of AMPK subunits at the mRNA level. We then analyzed the sex-specific AMPK activity by measuring the phosphorylation of the catalytic AMPK alpha protein and its expression at the protein level. Both the amount of AMPK alpha protein and threonine 172 phosphorylation appeared to be almost totally inhibited in mature female gonads at stage 3, at the time when accumulation of reserves in oocytes was promoted, while it remained at a high level in mature spermatozoa. Its activation might play a sex-dependent role in the management of energy during gametogenesis in oyster. |
author2 |
E., Guevelou A., Huvet C. E., Galindo Sanchez Milan, Massimo V., Quillien J. Y., Daniel C., Quere P., Boudry C., Corporeau |
format |
Article in Journal/Newspaper |
author |
E. Guevelou A. Huvet C. E. Galindo Sanchez V. Quillien J. Y. Daniel C. Quere P. Boudry C. Corporeau MILAN, MASSIMO |
spellingShingle |
E. Guevelou A. Huvet C. E. Galindo Sanchez V. Quillien J. Y. Daniel C. Quere P. Boudry C. Corporeau MILAN, MASSIMO Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas |
author_facet |
E. Guevelou A. Huvet C. E. Galindo Sanchez V. Quillien J. Y. Daniel C. Quere P. Boudry C. Corporeau MILAN, MASSIMO |
author_sort |
E. Guevelou |
title |
Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas |
title_short |
Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas |
title_full |
Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas |
title_fullStr |
Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas |
title_full_unstemmed |
Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas |
title_sort |
sex-specific regulation of amp-activated protein kinase (ampk) in the pacific oyster crassostrea gigas |
publishDate |
2013 |
url |
http://hdl.handle.net/11577/2715286 https://doi.org/10.1095/biolreprod.113.109728 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/23926284 info:eu-repo/semantics/altIdentifier/wos/WOS:000326925800019 volume:89 firstpage:100 lastpage:100 journal:BIOLOGY OF REPRODUCTION http://hdl.handle.net/11577/2715286 doi:10.1095/biolreprod.113.109728 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84899788469 |
op_doi |
https://doi.org/10.1095/biolreprod.113.109728 |
container_title |
Biology of Reproduction |
container_volume |
89 |
container_issue |
4 |
_version_ |
1796941105922572288 |