Evolutionary analysis of Antarctic teleost Toll-like receptor 2.
The nucleotide sequence of TLR2 has been determined in both species, encoding 20 leucine-rich repeats (LRRs) in the extracellular region and a classical Toll/IL-1R (TIR) domain in the intracellular region. High expression level of T. bernacchii TLR2 was found in spleen and skin. Using different meth...
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ftunivpadovairis:oai:www.research.unipd.it:11577/2525810 2024-02-11T09:58:14+01:00 Evolutionary analysis of Antarctic teleost Toll-like receptor 2. Varriale S Giacomelli S Coscia MR Oreste U. FERRARESSO, SERENA BARGELLONI, LUCA Varriale, S Ferraresso, Serena Giacomelli, S Coscia, Mr Bargelloni, Luca Oreste, U. 2012 STAMPA http://hdl.handle.net/11577/2525810 https://doi.org/10.1016/j.fsi.2012.07.010 eng eng info:eu-repo/semantics/altIdentifier/pmid/22960217 info:eu-repo/semantics/altIdentifier/wos/WOS:000311191700002 volume:33 firstpage:1076 lastpage:1085 journal:FISH AND SHELLFISH IMMUNOLOGY http://hdl.handle.net/11577/2525810 doi:10.1016/j.fsi.2012.07.010 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84872601879 info:eu-repo/semantics/article 2012 ftunivpadovairis https://doi.org/10.1016/j.fsi.2012.07.010 2024-01-17T17:39:05Z The nucleotide sequence of TLR2 has been determined in both species, encoding 20 leucine-rich repeats (LRRs) in the extracellular region and a classical Toll/IL-1R (TIR) domain in the intracellular region. High expression level of T. bernacchii TLR2 was found in spleen and skin. Using different methods we identified six codons that underwent Darwinian selection while 20 were found to be negatively selected. Molecular models of C. hamatus and T bernacchii TLR2 ectodomain as well as of the TIR domain were built by Homology Modeling. Molecular Dynamics simulations were performed in water for 15 ns. The sites under positive selection were residing on the convex side of the solenoid, four out of six were in a 35-residue-long region including the central/N-terminal domain boundary: two in the external loop of LRR11 and the other two in the LRR12 loop. This region has been demonstrated to be the functional site of ligand interaction in human TLR2 structure. Antarctic TLR2 models showed more flexibility than TLR2 from the temperate species Gasterosteus aculeatus. These results suggest that the selective pressure has shaped TLR2 molecule in such a way that increased its activity under the peculiar Antarctic environmental conditions. Article in Journal/Newspaper Antarc* Antarctic Padua Research Archive (IRIS - Università degli Studi di Padova) Antarctic Fish & Shellfish Immunology 33 5 1076 1085 |
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Open Polar |
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Padua Research Archive (IRIS - Università degli Studi di Padova) |
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ftunivpadovairis |
language |
English |
description |
The nucleotide sequence of TLR2 has been determined in both species, encoding 20 leucine-rich repeats (LRRs) in the extracellular region and a classical Toll/IL-1R (TIR) domain in the intracellular region. High expression level of T. bernacchii TLR2 was found in spleen and skin. Using different methods we identified six codons that underwent Darwinian selection while 20 were found to be negatively selected. Molecular models of C. hamatus and T bernacchii TLR2 ectodomain as well as of the TIR domain were built by Homology Modeling. Molecular Dynamics simulations were performed in water for 15 ns. The sites under positive selection were residing on the convex side of the solenoid, four out of six were in a 35-residue-long region including the central/N-terminal domain boundary: two in the external loop of LRR11 and the other two in the LRR12 loop. This region has been demonstrated to be the functional site of ligand interaction in human TLR2 structure. Antarctic TLR2 models showed more flexibility than TLR2 from the temperate species Gasterosteus aculeatus. These results suggest that the selective pressure has shaped TLR2 molecule in such a way that increased its activity under the peculiar Antarctic environmental conditions. |
author2 |
Varriale, S Ferraresso, Serena Giacomelli, S Coscia, Mr Bargelloni, Luca Oreste, U. |
format |
Article in Journal/Newspaper |
author |
Varriale S Giacomelli S Coscia MR Oreste U. FERRARESSO, SERENA BARGELLONI, LUCA |
spellingShingle |
Varriale S Giacomelli S Coscia MR Oreste U. FERRARESSO, SERENA BARGELLONI, LUCA Evolutionary analysis of Antarctic teleost Toll-like receptor 2. |
author_facet |
Varriale S Giacomelli S Coscia MR Oreste U. FERRARESSO, SERENA BARGELLONI, LUCA |
author_sort |
Varriale S |
title |
Evolutionary analysis of Antarctic teleost Toll-like receptor 2. |
title_short |
Evolutionary analysis of Antarctic teleost Toll-like receptor 2. |
title_full |
Evolutionary analysis of Antarctic teleost Toll-like receptor 2. |
title_fullStr |
Evolutionary analysis of Antarctic teleost Toll-like receptor 2. |
title_full_unstemmed |
Evolutionary analysis of Antarctic teleost Toll-like receptor 2. |
title_sort |
evolutionary analysis of antarctic teleost toll-like receptor 2. |
publishDate |
2012 |
url |
http://hdl.handle.net/11577/2525810 https://doi.org/10.1016/j.fsi.2012.07.010 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/22960217 info:eu-repo/semantics/altIdentifier/wos/WOS:000311191700002 volume:33 firstpage:1076 lastpage:1085 journal:FISH AND SHELLFISH IMMUNOLOGY http://hdl.handle.net/11577/2525810 doi:10.1016/j.fsi.2012.07.010 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84872601879 |
op_doi |
https://doi.org/10.1016/j.fsi.2012.07.010 |
container_title |
Fish & Shellfish Immunology |
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33 |
container_issue |
5 |
container_start_page |
1076 |
op_container_end_page |
1085 |
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1790593840332144640 |