Neurotransmitters and putative neuromediators in the gut of Anguilla anguilla (L.). Localizations in the enteric nervous and endocrine systems
The gut of silver eels (Anguilla anguilla L.) was investigated in order to describe both the cholinergic and adrenergic intramural innervations, and the localization of possible accessory neuromediators. Histochemical reactions for the demonstration of nicotinamide adenine dinucleotide phosphate, re...
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ftunivpadovairis:oai:www.research.unipd.it:11577/2468564 2024-04-14T08:01:00+00:00 Neurotransmitters and putative neuromediators in the gut of Anguilla anguilla (L.). Localizations in the enteric nervous and endocrine systems DOMENEGHINI C ARRIGHI S VEGGETTI A RADAELLI, GIUSEPPE MASCARELLO, FRANCESCO Domeneghini, C Radaelli, Giuseppe Arrighi, S Veggetti, A Mascarello, Francesco 2000 http://hdl.handle.net/11577/2468564 eng eng info:eu-repo/semantics/altIdentifier/pmid/11095101 info:eu-repo/semantics/altIdentifier/wos/WOS:000089286800009 volume:44 firstpage:295 lastpage:306 journal:EUROPEAN JOURNAL OF HISTOCHEMISTRY http://hdl.handle.net/11577/2468564 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0033758125 info:eu-repo/semantics/article 2000 ftunivpadovairis 2024-03-21T19:08:15Z The gut of silver eels (Anguilla anguilla L.) was investigated in order to describe both the cholinergic and adrenergic intramural innervations, and the localization of possible accessory neuromediators. Histochemical reactions for the demonstration of nicotinamide adenine dinucleotide phosphate, reduced form-(NADPH-)diaphorase and acetylcholinesterase (AChEase) were performed, as well as the immunohistochemical testing of tyrosine hydroxylase, met-enkephalin, substance P, calcitonin gene-related peptide (CGRP), bombesin, vasoactive intestinal peptide (VIP), neuropeptide Y (NPY), somatostatin, cholecystokinin-octapeptide (CCK-8), serotonin, cholineacetyl transferase. The results evidenced a different pattern in comparison with other vertebrates, namely mammals, and with other fish. Both NADPH-diaphorase and AChEase activities were histochemically detected all along the gut in the myenteric plexus, the inner musculature and the propria-submucosa. Tyrosine hydroxylase immunoreactivity was observed in the intestinal tract only, both in the myenteric plexus and in the inner musculature. Several neuropeptides (metenkephalin, CGRP, bombesin, substance P, VIP, NPY, somatostatin) were, in addition, detected in the intramural innervation; some of them also in epithelial cells of the diffuse endocrine system (met-enkephalin, substance P, NPY, somatostatin). Serotonin was only present in endocrine cells. Tyrosine hydroxylase immunoreactivity was present in localizations similar to those of NADPH-diaphorase-reactivity, and in the same nerve bundles in which substance P- and CGRP-like-immunoreactivities were detectable in the intestinal tract. In addition, NADPH-diaphorase-reactive neurons showed an anatomical relationship with AChEase-reactive nerve terminals, and a similar relationship existed between the latter and substance P-like immunoreactivity. Article in Journal/Newspaper Anguilla anguilla Padua Research Archive (IRIS - Università degli Studi di Padova) |
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Open Polar |
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Padua Research Archive (IRIS - Università degli Studi di Padova) |
op_collection_id |
ftunivpadovairis |
language |
English |
description |
The gut of silver eels (Anguilla anguilla L.) was investigated in order to describe both the cholinergic and adrenergic intramural innervations, and the localization of possible accessory neuromediators. Histochemical reactions for the demonstration of nicotinamide adenine dinucleotide phosphate, reduced form-(NADPH-)diaphorase and acetylcholinesterase (AChEase) were performed, as well as the immunohistochemical testing of tyrosine hydroxylase, met-enkephalin, substance P, calcitonin gene-related peptide (CGRP), bombesin, vasoactive intestinal peptide (VIP), neuropeptide Y (NPY), somatostatin, cholecystokinin-octapeptide (CCK-8), serotonin, cholineacetyl transferase. The results evidenced a different pattern in comparison with other vertebrates, namely mammals, and with other fish. Both NADPH-diaphorase and AChEase activities were histochemically detected all along the gut in the myenteric plexus, the inner musculature and the propria-submucosa. Tyrosine hydroxylase immunoreactivity was observed in the intestinal tract only, both in the myenteric plexus and in the inner musculature. Several neuropeptides (metenkephalin, CGRP, bombesin, substance P, VIP, NPY, somatostatin) were, in addition, detected in the intramural innervation; some of them also in epithelial cells of the diffuse endocrine system (met-enkephalin, substance P, NPY, somatostatin). Serotonin was only present in endocrine cells. Tyrosine hydroxylase immunoreactivity was present in localizations similar to those of NADPH-diaphorase-reactivity, and in the same nerve bundles in which substance P- and CGRP-like-immunoreactivities were detectable in the intestinal tract. In addition, NADPH-diaphorase-reactive neurons showed an anatomical relationship with AChEase-reactive nerve terminals, and a similar relationship existed between the latter and substance P-like immunoreactivity. |
author2 |
Domeneghini, C Radaelli, Giuseppe Arrighi, S Veggetti, A Mascarello, Francesco |
format |
Article in Journal/Newspaper |
author |
DOMENEGHINI C ARRIGHI S VEGGETTI A RADAELLI, GIUSEPPE MASCARELLO, FRANCESCO |
spellingShingle |
DOMENEGHINI C ARRIGHI S VEGGETTI A RADAELLI, GIUSEPPE MASCARELLO, FRANCESCO Neurotransmitters and putative neuromediators in the gut of Anguilla anguilla (L.). Localizations in the enteric nervous and endocrine systems |
author_facet |
DOMENEGHINI C ARRIGHI S VEGGETTI A RADAELLI, GIUSEPPE MASCARELLO, FRANCESCO |
author_sort |
DOMENEGHINI C |
title |
Neurotransmitters and putative neuromediators in the gut of Anguilla anguilla (L.). Localizations in the enteric nervous and endocrine systems |
title_short |
Neurotransmitters and putative neuromediators in the gut of Anguilla anguilla (L.). Localizations in the enteric nervous and endocrine systems |
title_full |
Neurotransmitters and putative neuromediators in the gut of Anguilla anguilla (L.). Localizations in the enteric nervous and endocrine systems |
title_fullStr |
Neurotransmitters and putative neuromediators in the gut of Anguilla anguilla (L.). Localizations in the enteric nervous and endocrine systems |
title_full_unstemmed |
Neurotransmitters and putative neuromediators in the gut of Anguilla anguilla (L.). Localizations in the enteric nervous and endocrine systems |
title_sort |
neurotransmitters and putative neuromediators in the gut of anguilla anguilla (l.). localizations in the enteric nervous and endocrine systems |
publishDate |
2000 |
url |
http://hdl.handle.net/11577/2468564 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/11095101 info:eu-repo/semantics/altIdentifier/wos/WOS:000089286800009 volume:44 firstpage:295 lastpage:306 journal:EUROPEAN JOURNAL OF HISTOCHEMISTRY http://hdl.handle.net/11577/2468564 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0033758125 |
_version_ |
1796306094781366272 |