Identification of alpha 2U-globulin and bound volatiles in the Indian common house rat (Rattus rattus)
The alpha2u-globulin (alpha2u) is a pheromone carrier urinary protein believed to be relevant for sexual communication among rats and is characterized in laboratory rats. In the present study 17 kDa protein and the bound pheromones were characterized in a population of wild-type Indian common house...
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ftunivpadovairis:oai:www.research.unipd.it:11577/2463172 2024-02-27T08:45:03+00:00 Identification of alpha 2U-globulin and bound volatiles in the Indian common house rat (Rattus rattus) RAJKUMAR R HAYARAJA R ARCHUNAN G. MUCIGNAT, CARLA CAVAGGIONI, ANDREA Rajkumar, R Hayaraja, R Mucignat, Carla Cavaggioni, Andrea Archunan, G. 2009 STAMPA http://hdl.handle.net/11577/2463172 eng eng info:eu-repo/semantics/altIdentifier/pmid/19788064 info:eu-repo/semantics/altIdentifier/wos/WOS:000270005700006 volume:46 firstpage:319 lastpage:324 numberofpages:6 journal:INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS http://hdl.handle.net/11577/2463172 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84924247705 info:eu-repo/semantics/article 2009 ftunivpadovairis 2024-01-31T17:40:43Z The alpha2u-globulin (alpha2u) is a pheromone carrier urinary protein believed to be relevant for sexual communication among rats and is characterized in laboratory rats. In the present study 17 kDa protein and the bound pheromones were characterized in a population of wild-type Indian common house rat (Rattus rattus). The protein was purified by two runs of Sephadex G-50 chromatography and analyzed with SDS-PAGE with MALDI-TOF/MS. The results of MASCOT search identified the protein as an alpha2u and suggested a role for binding pheromones. To confirm the protein bound volatiles, purified alpha2u was extracted with dichloromethane and volatile molecules were detected using of gas chromatography linked to mass spectrometry (GC-MS). 1-Chlorodecane was detected as the predominant compound and 2-methyl-N-phenyl-2-propenamide, hexadecane and 2,6,11-trimethyl decane as the minor compounds. The simple method of protein purification and the identification of bound volatiles may help in designing efficient pheromone-based rat traps. Article in Journal/Newspaper Rattus rattus Padua Research Archive (IRIS - Università degli Studi di Padova) Indian |
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Open Polar |
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Padua Research Archive (IRIS - Università degli Studi di Padova) |
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ftunivpadovairis |
language |
English |
description |
The alpha2u-globulin (alpha2u) is a pheromone carrier urinary protein believed to be relevant for sexual communication among rats and is characterized in laboratory rats. In the present study 17 kDa protein and the bound pheromones were characterized in a population of wild-type Indian common house rat (Rattus rattus). The protein was purified by two runs of Sephadex G-50 chromatography and analyzed with SDS-PAGE with MALDI-TOF/MS. The results of MASCOT search identified the protein as an alpha2u and suggested a role for binding pheromones. To confirm the protein bound volatiles, purified alpha2u was extracted with dichloromethane and volatile molecules were detected using of gas chromatography linked to mass spectrometry (GC-MS). 1-Chlorodecane was detected as the predominant compound and 2-methyl-N-phenyl-2-propenamide, hexadecane and 2,6,11-trimethyl decane as the minor compounds. The simple method of protein purification and the identification of bound volatiles may help in designing efficient pheromone-based rat traps. |
author2 |
Rajkumar, R Hayaraja, R Mucignat, Carla Cavaggioni, Andrea Archunan, G. |
format |
Article in Journal/Newspaper |
author |
RAJKUMAR R HAYARAJA R ARCHUNAN G. MUCIGNAT, CARLA CAVAGGIONI, ANDREA |
spellingShingle |
RAJKUMAR R HAYARAJA R ARCHUNAN G. MUCIGNAT, CARLA CAVAGGIONI, ANDREA Identification of alpha 2U-globulin and bound volatiles in the Indian common house rat (Rattus rattus) |
author_facet |
RAJKUMAR R HAYARAJA R ARCHUNAN G. MUCIGNAT, CARLA CAVAGGIONI, ANDREA |
author_sort |
RAJKUMAR R |
title |
Identification of alpha 2U-globulin and bound volatiles in the Indian common house rat (Rattus rattus) |
title_short |
Identification of alpha 2U-globulin and bound volatiles in the Indian common house rat (Rattus rattus) |
title_full |
Identification of alpha 2U-globulin and bound volatiles in the Indian common house rat (Rattus rattus) |
title_fullStr |
Identification of alpha 2U-globulin and bound volatiles in the Indian common house rat (Rattus rattus) |
title_full_unstemmed |
Identification of alpha 2U-globulin and bound volatiles in the Indian common house rat (Rattus rattus) |
title_sort |
identification of alpha 2u-globulin and bound volatiles in the indian common house rat (rattus rattus) |
publishDate |
2009 |
url |
http://hdl.handle.net/11577/2463172 |
geographic |
Indian |
geographic_facet |
Indian |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/19788064 info:eu-repo/semantics/altIdentifier/wos/WOS:000270005700006 volume:46 firstpage:319 lastpage:324 numberofpages:6 journal:INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS http://hdl.handle.net/11577/2463172 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84924247705 |
_version_ |
1792054124344246272 |